Chih-Ching Yen1,2,3, Wen-Hui Chang1, Min-Che Tung4,5, Hsiao-Ling Chen6, Hsu-Chung Liu1,7, Chun-Huei Liao1, Ying-Wei Lan1,8, Kowit-Yu Chong8,9, Shang-Hsun Yang10, Chuan-Mu Chen11,12,13. 1. Department of Life Sciences, National Chung Hsing University, Taichung, 402, Taiwan. 2. Department of Internal Medicine, China Medical University Hospital, Taichung, 404, Taiwan. 3. College of Health Care, China Medical University, Taichung, 404, Taiwan. 4. Department of Surgery, Tungs' Taichung Metro Harbor Hospital, Taichung, 435, Taiwan. 5. Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, 110, Taiwan. 6. Department of Bioresources, Da-Yeh University, Changhua, 515, Taiwan. 7. Division of Chest Medicine, Department of Internal Medicine, Cheng Ching Hospital, Taichung, 404, Taiwan. 8. Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Tao-Yuan, 333, Taiwan. 9. Department of Thoracic Medicine, Chang Gung Memorial Hospital at Linkou, Tao-Yuan, 333, Taiwan. 10. Department of Physiology, and Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, 701, Taiwan. 11. Department of Life Sciences, National Chung Hsing University, Taichung, 402, Taiwan. chchen1@dragon.nchu.edu.tw. 12. The iEGG and Animal Biotechnology Center, and Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, 402, Taiwan. chchen1@dragon.nchu.edu.tw. 13. Department of Life Sciences, College of Life Sciences, National Chung Hsing University, No. 250, Kuo Kuang Rd, Taichung, 402, Taiwan. chchen1@dragon.nchu.edu.tw.
Abstract
PURPOSE: High levels of oxygen are usually used in ventilatory support and extracorporeal membrane oxygenation (ECMO) in the intensive care unit of hospitals. Hyperoxia may induce the production of reactive oxygen species (ROS) that can cause lung damage and even systemic injury. In this study, the NF-κB/luciferase transgenic mouse model with non-invasive real-time in vivo imaging was established to test the functions of lactoferrin (LF) in antioxidant and anti-inflammation. PROCEDURES: The NF-κB/luciferase transgenic mice were used to assess the effects of oral administration of LF on attenuation of the systemic inflammatory response and organ damage after 72 h of hyperoxia (FiO2 > 95 %) exposure via monitoring using an in vivo imaging system (IVIS). RESULTS: Using luciferase IVIS imaging, we found that the lungs and kidneys were the most evidently affected organs after hyperoxia treatment. The groups treated with low dose (150 mg/kg) or high dose (300 mg/kg) of LF had lower luciferase expression and less injury, with a dose-dependent effect on the lungs and kidneys. Moreover, ROS, mitogen-activated protein kinases (MAPK), and pro-inflammatory cytokine (TNF-α, IL-1ß, and IL-6) expression levels were all significantly decreased (P < 0.01), and the protein level of IκB was statistically increased (P < 0.01) after LF treatment. CONCLUSIONS: Our results suggest that hyperoxia can induce systemic inflammation, and the oral administration of LF as a natural antioxidant decreases the production of ROS, attenuates inflammation, and lessens kidney and lung injuries from hyperoxia via the use of live image monitoring of the response in NF-kB/luciferase transgenic mice.
PURPOSE: High levels of oxygen are usually used in ventilatory support and extracorporeal membrane oxygenation (ECMO) in the intensive care unit of hospitals. Hyperoxia may induce the production of reactive oxygen species (ROS) that can cause lung damage and even systemic injury. In this study, the NF-κB/luciferase transgenicmouse model with non-invasive real-time in vivo imaging was established to test the functions of lactoferrin (LF) in antioxidant and anti-inflammation. PROCEDURES: The NF-κB/luciferase transgenic mice were used to assess the effects of oral administration of LF on attenuation of the systemic inflammatory response and organ damage after 72 h of hyperoxia (FiO2 > 95 %) exposure via monitoring using an in vivo imaging system (IVIS). RESULTS: Using luciferase IVIS imaging, we found that the lungs and kidneys were the most evidently affected organs after hyperoxia treatment. The groups treated with low dose (150 mg/kg) or high dose (300 mg/kg) of LF had lower luciferase expression and less injury, with a dose-dependent effect on the lungs and kidneys. Moreover, ROS, mitogen-activated protein kinases (MAPK), and pro-inflammatory cytokine (TNF-α, IL-1ß, and IL-6) expression levels were all significantly decreased (P < 0.01), and the protein level of IκB was statistically increased (P < 0.01) after LF treatment. CONCLUSIONS: Our results suggest that hyperoxia can induce systemic inflammation, and the oral administration of LF as a natural antioxidant decreases the production of ROS, attenuates inflammation, and lessens kidney and lung injuries from hyperoxia via the use of live image monitoring of the response in NF-kB/luciferase transgenic mice.
Entities:
Keywords:
Inflammatory cytokines; Lactoferrin (LF); Live imaging; NF-κB/luciferase transgenic mice; Reactive oxygen species (ROS)
Authors: Jonathan Himmelfarb; Ellen McMonagle; Stephanie Freedman; Jennifer Klenzak; Elizabeth McMenamin; Phuong Le; Lara B Pupim; T Alp Ikizler Journal: J Am Soc Nephrol Date: 2004-09 Impact factor: 10.121
Authors: Fred Rincon; Joon Kang; Mitchell Maltenfort; Matthew Vibbert; Jacqueline Urtecho; M Kamran Athar; Jack Jallo; Carissa C Pineda; Diana Tzeng; William McBride; Rodney Bell Journal: Crit Care Med Date: 2014-02 Impact factor: 7.598
Authors: Evert de Jonge; Linda Peelen; Peter J Keijzers; Hans Joore; Dylan de Lange; Peter H J van der Voort; Robert J Bosman; Ruud A L de Waal; Ronald Wesselink; Nicolette F de Keizer Journal: Crit Care Date: 2008-12-10 Impact factor: 9.097