OBJECTIVE: To analyze the relationship between CDKN2A and CDKN2B gene methylation with aging in the general population. METHODS: We collected peripheral blood samples from 284 male and 246 female healthy subjects for detection of methylation levels of CDKN2A and CDKN2B genes using quantitative methylation-specific PCR (qMSP). The relationship between the methylation levels of CDKN2A and CDKN2B genes and aging was analyzed using Spearman or Pearson correlation test. RESULTS: We found a significant positive correlation between the methylation levels of the two genes in these subjects (P < 0.05). In the overall population as well in the female subjects, CDKN2A methylation was found to be inversely correlated with age (P < 0.05). The methylation levels of CDKN2A and CDKN2B genes were inversely correlated with TG, ApoE, Lp(a) and AST in the overall population (P < 0.05). In both the female and male subjects, the methylation levels of the two genes were inversely correlated with Lp(a) (P < 0.05). In the male subjects, CDKN2A methylation was inversely correlated with AST (P < 0.05), while CDKN2B methylation was inversely correlated with HDL and ApoE (P < 0.05). In the female subjects, CDKN2A methylation was positively correlated with LDL and inversely correlated with ApoE and AST (P < 0.05). CONCLUSIONS: The methylation levels of CDKN2A and CDKN2B are closely related to age and the levels of multiple proteins in healthy subjects.
OBJECTIVE: To analyze the relationship between CDKN2A and CDKN2B gene methylation with aging in the general population. METHODS: We collected peripheral blood samples from 284 male and 246 female healthy subjects for detection of methylation levels of CDKN2A and CDKN2B genes using quantitative methylation-specific PCR (qMSP). The relationship between the methylation levels of CDKN2A and CDKN2B genes and aging was analyzed using Spearman or Pearson correlation test. RESULTS: We found a significant positive correlation between the methylation levels of the two genes in these subjects (P &lt; 0.05). In the overall population as well in the female subjects, CDKN2A methylation was found to be inversely correlated with age (P &lt; 0.05). The methylation levels of CDKN2A and CDKN2B genes were inversely correlated with TG, ApoE, Lp(a) and AST in the overall population (P &lt; 0.05). In both the female and male subjects, the methylation levels of the two genes were inversely correlated with Lp(a) (P &lt; 0.05). In the male subjects, CDKN2A methylation was inversely correlated with AST (P &lt; 0.05), while CDKN2B methylation was inversely correlated with HDL and ApoE (P &lt; 0.05). In the female subjects, CDKN2A methylation was positively correlated with LDL and inversely correlated with ApoE and AST (P &lt; 0.05). CONCLUSIONS: The methylation levels of CDKN2A and CDKN2B are closely related to age and the levels of multiple proteins in healthy subjects.
Authors: Mary K Keyes; Hyeran Jang; Joel B Mason; Zhenhua Liu; Jimmy W Crott; Donald E Smith; Simonetta Friso; Sang-Woon Choi Journal: J Nutr Date: 2007-07 Impact factor: 4.798