| Literature DB >> 31267316 |
Changchang Meng1, Jinyan Zhang2, Luyu Zhang3, Yueting Wang1, Zhenyu Li2, Jing Zhao4.
Abstract
The NOD-like receptor protein 6 (NLRP6), an intracytoplasmic pattern recognition receptor in the nucleotide-binding domain, leucine-rich repeat-containing (NLR) innate immune receptor family, influences the inflammation reaction. The role of NLRP6 in cerebral ischemia-reperfusion (I/R) injury in rats is unclear. We explore the function of NLRP6 in cerebral I/R injury. The investigators used a middle cerebral artery occlusion/reperfusion model (MCAO) to imitate ischemic injury. We found the peak expression of NLRP6 is in 48-h post-cerebral I/R injury. The expression of NLRP6 siRNA, as well as the expression of protein and mRNA, was detected by Western blot and qRT-PCR. The degree of IL-1β and IL-18 was assessed by ELISA. After downregulating NLRP6, the expression of IL-1β, IL-18, cleaved Caspase-1, and myeloperoxidase (MPO) were reduced. In HE and Nissl staining, pathological injury of brain tissue after downregulating NLRP6 was improved. NLRP6 siRNA decreased the NLRP6-ASC binding states by CO-IP. NRP6 has a pro-inflammatory effect in cerebral I/R injury, which may provide a new target for the treatment of cerebral I/R injury.Entities:
Keywords: Cerebral ischemia/reperfusion injury; IL (interleukin)-18; IL (interleukin)-1β; Inflammation; NLRP6
Mesh：
Substances：
Year: 2019 PMID： 31267316 DOI： 10.1007/s12031-019-01370-4
Source DB: PubMed Journal: J Mol Neurosci ISSN： 0895-8696 Impact factor: 3.444