Literature DB >> 31250300

Three-Dimensional Surface Rendering of ESCRT Proteins Microscopy Data Using UCSF Chimera Software.

Romain Le Bars1, Michele W Bianchi2,3, Christophe Lefebvre2.   

Abstract

Visualization of subcellular localization of ESCRT proteins and their interactions with different cellular compartments are critical to understand their function. This approach requires the generation of an important amount of 3D fluorescence microscopy data that is not always easy to visualize and apprehend.We describe a step-by-step protocol for 3D surface rendering of confocal microscopy acquisitions using the free software UCSF-Chimera, generating snapshots and animations to facilitate analysis and presentation of subcellular localization data.

Entities:  

Keywords:  3D animation; 3D fluorescence confocal microscopy; ESCRT; Surface rendering; UCSF Chimera

Mesh：

Substances：

Year:  2019        PMID： 31250300     DOI： 10.1007/978-1-4939-9492-2_11

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

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Journal:  Cell       Date:  2001-10-05       Impact factor: 41.582

2.  UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.

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Journal:  J Cell Sci       Date:  2013-10-08       Impact factor: 5.285

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5.  ESCRT machinery is required for plasma membrane repair.

Authors:  Ana Joaquina Jimenez; Paolo Maiuri; Julie Lafaurie-Janvore; Séverine Divoux; Matthieu Piel; Franck Perez
Journal:  Science       Date:  2014-01-30       Impact factor: 47.728

Review 6.  Membrane fission reactions of the mammalian ESCRT pathway.

Authors:  John McCullough; Leremy A Colf; Wesley I Sundquist
Journal:  Annu Rev Biochem       Date:  2013-03-18       Impact factor: 23.643

7.  Surveillance of nuclear pore complex assembly by ESCRT-III/Vps4.

Authors:  Brant M Webster; Paolo Colombi; Jens Jäger; C Patrick Lusk
Journal:  Cell       Date:  2014-10-09       Impact factor: 41.582

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Authors:  Jez G Carlton; Juan Martin-Serrano
Journal:  Science       Date:  2007-06-07       Impact factor: 47.728

9.  ESCRT-III controls nuclear envelope reformation.

Authors:  Yolanda Olmos; Lorna Hodgson; Judith Mantell; Paul Verkade; Jeremy G Carlton
Journal:  Nature       Date:  2015-06-03       Impact factor: 49.962

10.  Polarized release of T-cell-receptor-enriched microvesicles at the immunological synapse.

Authors:  Kaushik Choudhuri; Jaime Llodrá; Eric W Roth; Jones Tsai; Susana Gordo; Kai W Wucherpfennig; Lance C Kam; David L Stokes; Michael L Dustin
Journal:  Nature       Date:  2014-02-02       Impact factor: 49.962
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Journal:  Appl Microbiol Biotechnol       Date:  2021-02-01       Impact factor: 4.813

2.  Evaluation of the Mechanism of Jiedu Huazhuo Quyu Formula in Treating Wilson's Disease-Associated Liver Fibrosis by Network Pharmacology Analysis and Molecular Dynamics Simulation.

Authors:  Shao-Peng Huang; Sen Chen; Yan-Zhen Ma; An Zhou; Hui Jiang; Peng Wu
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3.  Prediction of the RNA Tertiary Structure Based on a Random Sampling Strategy and Parallel Mechanism.

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  3 in total

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