Literature DB >> 31250294

Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected Cells.

Binod Kumar1, Mohanan Valiya Veettil1,2, Arunava Roy1,3, Bala Chandran4,5.   

Abstract

Proximity ligation assay (PLA) is a newly developed technique that outperforms the traditional immunoassays for visualizing the in situ endogenous protein-protein interactions and localizations and the activation of proteins in cell culture systems as well as in tissue sections. PLA, when combined with cellular marker staining, becomes a powerful approach to identify differential interaction of the proteins of endosomal sorting complex required for transport (ESCRT) at distinct stages of virus infection. In this chapter, we describe a PLA protocol to study the localization and interaction between the ESCRT protein TSG101 and endosomal markers in early stages of viral endocytosis in in vitro infected cells.

Entities:  

Keywords:  Colocalization; ESCRT; Endocytosis; Proximity ligation assay; Trafficking; Virus infection

Mesh：

Substances：

Year:  2019        PMID： 31250294     DOI： 10.1007/978-1-4939-9492-2_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  Protein detection using proximity-dependent DNA ligation assays.

Authors:  Simon Fredriksson; Mats Gullberg; Jonas Jarvius; Charlotta Olsson; Kristian Pietras; Sigrún Margrét Gústafsdóttir; Arne Ostman; Ulf Landegren
Journal:  Nat Biotechnol       Date:  2002-05       Impact factor: 54.908

2.  Solid-phase proximity ligation assays for individual or parallel protein analyses with readout via real-time PCR or sequencing.

Authors:  Rachel Yuan Nong; Di Wu; Junhong Yan; Maria Hammond; Gucci Jijuan Gu; Masood Kamali-Moghaddam; Ulf Landegren; Spyros Darmanis
Journal:  Nat Protoc       Date:  2013-05-30       Impact factor: 13.491

3.  Direct observation of individual endogenous protein complexes in situ by proximity ligation.

Authors:  Ola Söderberg; Mats Gullberg; Malin Jarvius; Karin Ridderstråle; Karl-Johan Leuchowius; Jonas Jarvius; Kenneth Wester; Per Hydbring; Fuad Bahram; Lars-Gunnar Larsson; Ulf Landegren
Journal:  Nat Methods       Date:  2006-10-29       Impact factor: 28.547

4.  ESCRT-0 Component Hrs Promotes Macropinocytosis of Kaposi's Sarcoma-Associated Herpesvirus in Human Dermal Microvascular Endothelial Cells.

Authors:  Mohanan Valiya Veettil; Binod Kumar; Mairaj Ahmed Ansari; Dipanjan Dutta; Jawed Iqbal; Olsi Gjyshi; Virginie Bottero; Bala Chandran
Journal:  J Virol       Date:  2016-03-28       Impact factor: 5.103

5.  Direct visualization of newly synthesized target proteins in situ.

Authors:  Susanne tom Dieck; Lisa Kochen; Cyril Hanus; Maximilian Heumüller; Ina Bartnik; Belquis Nassim-Assir; Katrin Merk; Thorsten Mosler; Sakshi Garg; Stefanie Bunse; David A Tirrell; Erin M Schuman
Journal:  Nat Methods       Date:  2015-03-16       Impact factor: 28.547

6.  Kaposi's Sarcoma Associated Herpesvirus Entry into Target Cells.

Authors:  Sayan Chakraborty; Mohanan Valiya Veettil; Bala Chandran
Journal:  Front Microbiol       Date:  2012-01-20       Impact factor: 5.640

Review 7.  Interaction of KSHV with host cell surface receptors and cell entry.

Authors:  Mohanan Valiya Veettil; Chirosree Bandyopadhyay; Dipanjan Dutta; Bala Chandran
Journal:  Viruses       Date:  2014-10-23       Impact factor: 5.048

8.  ESCRT-I Protein Tsg101 Plays a Role in the Post-macropinocytic Trafficking and Infection of Endothelial Cells by Kaposi's Sarcoma-Associated Herpesvirus.

Authors:  Binod Kumar; Dipanjan Dutta; Jawed Iqbal; Mairaj Ahmed Ansari; Arunava Roy; Leela Chikoti; Gina Pisano; Mohanan Valiya Veettil; Bala Chandran
Journal:  PLoS Pathog       Date:  2016-10-20       Impact factor: 6.823

Review 9.  KSHV Entry and Trafficking in Target Cells-Hijacking of Cell Signal Pathways, Actin and Membrane Dynamics.

Authors:  Binod Kumar; Bala Chandran
Journal:  Viruses       Date:  2016-11-14       Impact factor: 5.048

10.  CIB1 synergizes with EphrinA2 to regulate Kaposi's sarcoma-associated herpesvirus macropinocytic entry in human microvascular dermal endothelial cells.

Authors:  Chirosree Bandyopadhyay; Mohanan Valiya-Veettil; Dipanjan Dutta; Sayan Chakraborty; Bala Chandran
Journal:  PLoS Pathog       Date:  2014-02-13       Impact factor: 6.823
  10 in total
  3 in total

Review 1.  Proximity ligation assay: an ultrasensitive method for protein quantification and its applications in pathogen detection.

Authors:  Pengzhi Wang; Yi Yang; Tianqi Hong; Guoqiang Zhu
Journal:  Appl Microbiol Biotechnol       Date:  2021-01-11       Impact factor: 4.813

2.  Extrachromosomal Histone H2B Contributes to the Formation of the Abscission Site for Cell Division.

Authors:  Laura Monteonofrio; Davide Valente; Cinzia Rinaldo; Silvia Soddu
Journal:  Cells       Date:  2019-11-05       Impact factor: 6.600

3.  Toxoplasma gondii exploits the host ESCRT machinery for parasite uptake of host cytosolic proteins.

Authors:  Yolanda Rivera-Cuevas; Joshua Mayoral; Manlio Di Cristina; Anna-Lisa E Lawrence; Einar B Olafsson; Romir K Patel; Dishari Thornhill; Benjamin S Waldman; Akira Ono; Jonathan Z Sexton; Sebastian Lourido; Louis M Weiss; Vern B Carruthers
Journal:  PLoS Pathog       Date:  2021-12-13       Impact factor: 7.464
  3 in total

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