Literature DB >> 25775042

Direct visualization of newly synthesized target proteins in situ.

Susanne tom Dieck1, Lisa Kochen1, Cyril Hanus1, Maximilian Heumüller1, Ina Bartnik1, Belquis Nassim-Assir1, Katrin Merk1, Thorsten Mosler1, Sakshi Garg1, Stefanie Bunse1, David A Tirrell2, Erin M Schuman1.   

Abstract

Protein synthesis is a dynamic process that tunes the cellular proteome in response to internal and external demands. Metabolic labeling approaches identify the general proteomic response but cannot visualize specific newly synthesized proteins within cells. Here we describe a technique that couples noncanonical amino acid tagging or puromycylation with the proximity ligation assay to visualize specific newly synthesized proteins and monitor their origin, redistribution and turnover in situ.

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Year:  2015        PMID： 25775042      PMCID： PMC4414919          DOI： 10.1038/nmeth.3319

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  27 in total

1.  Dynamic visualization of local protein synthesis in hippocampal neurons.

Authors:  G Aakalu; W B Smith; N Nguyen; C Jiang; E M Schuman
Journal:  Neuron       Date:  2001-05       Impact factor: 17.173

2.  Assembly of active zone precursor vesicles: obligatory trafficking of presynaptic cytomatrix proteins Bassoon and Piccolo via a trans-Golgi compartment.

Authors:  Thomas Dresbach; Viviana Torres; Nina Wittenmayer; Wilko D Altrock; Pedro Zamorano; Werner Zuschratter; Ralph Nawrotzki; Noam E Ziv; Craig C Garner; Eckart D Gundelfinger
Journal:  J Biol Chem       Date:  2005-12-21       Impact factor: 5.157

Review 3.  Inhibitors of ribosome functions.

Authors:  S Pestka
Journal:  Annu Rev Microbiol       Date:  1971       Impact factor: 15.500

4.  Direct observation of individual endogenous protein complexes in situ by proximity ligation.

Authors:  Ola Söderberg; Mats Gullberg; Malin Jarvius; Karin Ridderstråle; Karl-Johan Leuchowius; Jonas Jarvius; Kenneth Wester; Per Hydbring; Fuad Bahram; Lars-Gunnar Larsson; Ulf Landegren
Journal:  Nat Methods       Date:  2006-10-29       Impact factor: 28.547

5.  In situ detection of phosphorylated platelet-derived growth factor receptor beta using a generalized proximity ligation method.

Authors:  Malin Jarvius; Janna Paulsson; Irene Weibrecht; Karl-Johan Leuchowius; Ann-Catrin Andersson; Carolina Wählby; Mats Gullberg; Johan Botling; Tobias Sjöblom; Boyka Markova; Arne Ostman; Ulf Landegren; Ola Söderberg
Journal:  Mol Cell Proteomics       Date:  2007-06-12       Impact factor: 5.911

6.  Preparation of the functionalizable methionine surrogate azidohomoalanine via copper-catalyzed diazo transfer.

Authors:  A James Link; Mandy K S Vink; David A Tirrell
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

7.  Subcellular profiling reveals distinct and developmentally regulated repertoire of growth cone mRNAs.

Authors:  Krishna H Zivraj; Yi Chun Loraine Tung; Michael Piper; Laura Gumy; James W Fawcett; Giles S H Yeo; Christine E Holt
Journal:  J Neurosci       Date:  2010-11-17       Impact factor: 6.167

8.  Metabolic turnover of synaptic proteins: kinetics, interdependencies and implications for synaptic maintenance.

Authors:  Laurie D Cohen; Rina Zuchman; Oksana Sorokina; Anke Müller; Daniela C Dieterich; J Douglas Armstrong; Tamar Ziv; Noam E Ziv
Journal:  PLoS One       Date:  2013-05-02       Impact factor: 3.240

9.  Nuclear translation visualized by ribosome-bound nascent chain puromycylation.

Authors:  Alexandre David; Brian P Dolan; Heather D Hickman; Jonathan J Knowlton; Giovanna Clavarino; Philippe Pierre; Jack R Bennink; Jonathan W Yewdell
Journal:  J Cell Biol       Date:  2012-04-02       Impact factor: 10.539

10.  Metabolic labeling with noncanonical amino acids and visualization by chemoselective fluorescent tagging.

Authors:  Susanne Tom Dieck; Anke Müller; Anne Nehring; Flora I Hinz; Ina Bartnik; Erin M Schuman; Daniela C Dieterich
Journal:  Curr Protoc Cell Biol       Date:  2012-09
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  112 in total

Review 1.  The Regulation of Synaptic Protein Turnover.

Authors:  Beatriz Alvarez-Castelao; Erin M Schuman
Journal:  J Biol Chem       Date:  2015-10-09       Impact factor: 5.157

Review 2.  Cell-selective proteomics for biological discovery.

Authors:  Shannon E Stone; Weslee S Glenn; Graham D Hamblin; David A Tirrell
Journal:  Curr Opin Chem Biol       Date:  2017-01-12       Impact factor: 8.822

3.  THE AUTHOR FILE: Erin Margaret Schuman.

Authors:  Vivien Marx
Journal:  Nat Methods       Date:  2015-05       Impact factor: 28.547

4.  Glutamate Receptor Trafficking and Protein Synthesis Mediate the Facilitation of LTP by Secreted Amyloid Precursor Protein-Alpha.

Authors:  Bruce G Mockett; Diane Guévremont; Megan K Elder; Karen D Parfitt; Katie Peppercorn; Jodi Morrissey; Anurag Singh; Timothy J Hintz; Lisa Kochen; Susanne Tom Dieck; Erin Schuman; Warren P Tate; Joanna M Williams; Wickliffe C Abraham
Journal:  J Neurosci       Date:  2019-02-25       Impact factor: 6.167

Review 5.  Examining the evidence for extracellular RNA function in mammals.

Authors:  Hannah N Gruner; Michael T McManus
Journal:  Nat Rev Genet       Date:  2021-04-06       Impact factor: 53.242

Review 6.  Local translation in neuronal compartments: how local is local?

Authors:  Vidhya Rangaraju; Susanne Tom Dieck; Erin M Schuman
Journal:  EMBO Rep       Date:  2017-04-12       Impact factor: 8.807

7.  Visualizing Local Protein Synthesis and Its Modulation by FMRP and Visual Experience.

Authors:  Gervasio Batista; Hannah R Monday
Journal:  J Neurosci       Date:  2016-11-23       Impact factor: 6.167

8.  Squid Giant Axon Contains Neurofilament Protein mRNA but does not Synthesize Neurofilament Proteins.

Authors:  Harold Gainer; Shirley House; Dong Sun Kim; Hemin Chin; Harish C Pant
Journal:  Cell Mol Neurobiol       Date:  2016-05-20       Impact factor: 5.046

9.  Somatodendritic accumulation of Tau in Alzheimer's disease is promoted by Fyn-mediated local protein translation.

Authors:  Chuanzhou Li; Jürgen Götz
Journal:  EMBO J       Date:  2017-09-01       Impact factor: 11.598

Review 10.  The Growing Toolbox for Protein Synthesis Studies.

Authors:  Shintaro Iwasaki; Nicholas T Ingolia
Journal:  Trends Biochem Sci       Date:  2017-05-28       Impact factor: 13.807
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