Mohammad R Daremipouran1, Desta Beyene1, Victor Apprey2, Tammey J Naab3, Olakunle O Kassim4, Robert L Copeland1,5, Yasmine M Kanaan6,4. 1. Cancer Center, Howard University, Washington, DC, U.S.A. 2. Community and Family Medicine, College of Medicine, Howard University, Washington, DC, U.S.A. 3. Department of Pathology, College of Medicine, Howard University, Washington, DC, U.S.A. 4. Department of Microbiology, College of Medicine, Howard University, Washington, DC, U.S.A. 5. Department of Pharmacology, College of Medicine, Howard University, Washington, DC, U.S.A. 6. Cancer Center, Howard University, Washington, DC, U.S.A. ymkanaan@howard.edu.
Abstract
BACKGROUND/AIM: Vitamin D receptor (VDR) is present in numerous cellular pathways and it has been suggested that VDR genetic variants influence individual susceptibility to prostate cancer. Also, analyses of single nucleotide polymorphisms (SNPs) in VDR revealed ethnicity-associated polymorphisms. The aim of this study was to identify VDR SNPs in African American men with and without prostate cancer. MATERIALS AND METHODS: The entire VDR gene was screened for germline mutations in a case-control study by denaturing high performance liquid chromatography and DNA sequencing. Logistic regression was used to estimate the association of SNPs, age, family history, and Gleason score with prostate cancer risk. RESULTS: Six SNPs in the non-coding regions, and one SNP in the coding region, were detected. SNP 1 (c.278-69G>A) and SNP 4 (c.907+75C>T) have not been previously reported. SNP 4 had a significant protective effect (β=-0.6, p<0.05); whereas, SNP 7 (rs7975232) showed an increase association with prostate cancer risk and high Gleason score (β=0.32, p<0.05). SNP 4, SNP 7 and age were better predictors of prostate cancer risk than family history with a high degree of sensitivity (74.7%) and specificity (92.4%). CONCLUSION: SNP 4 and SNP 7 could be promising markers for prediction of reduced or increased prostate cancer risk, respectively. Copyright
BACKGROUND/AIM: Vitamin D receptor (VDR) is present in numerous cellular pathways and it has been suggested that VDR genetic variants influence individual susceptibility to prostate cancer. Also, analyses of single nucleotide polymorphisms (SNPs) in VDR revealed ethnicity-associated polymorphisms. The aim of this study was to identify VDR SNPs in African American men with and without prostate cancer. MATERIALS AND METHODS: The entire VDR gene was screened for germline mutations in a case-control study by denaturing high performance liquid chromatography and DNA sequencing. Logistic regression was used to estimate the association of SNPs, age, family history, and Gleason score with prostate cancer risk. RESULTS: Six SNPs in the non-coding regions, and one SNP in the coding region, were detected. SNP 1 (c.278-69G>A) and SNP 4 (c.907+75C>T) have not been previously reported. SNP 4 had a significant protective effect (β=-0.6, p<0.05); whereas, SNP 7 (rs7975232) showed an increase association with prostate cancer risk and high Gleason score (β=0.32, p<0.05). SNP 4, SNP 7 and age were better predictors of prostate cancer risk than family history with a high degree of sensitivity (74.7%) and specificity (92.4%). CONCLUSION: SNP 4 and SNP 7 could be promising markers for prediction of reduced or increased prostate cancer risk, respectively. Copyright
Authors: Desta A Beyene; Mohammad R Daremipouran; Victor Apprey; Tammey Naab; Olakunle O Kassim; Robert L Copeland; Yasmine M Kanaan Journal: Cancer Genomics Proteomics Date: 2020 Nov-Dec Impact factor: 4.069
Authors: Shu-Hong Lin; Joshua N Sampson; Thomas G P Grünewald; Didier Surdez; Stephanie Reynaud; Olivier Mirabeau; Eric Karlins; Rebeca Alba Rubio; Sakina Zaidi; Sandrine Grossetête-Lalami; Stelly Ballet; Eve Lapouble; Valérie Laurence; Jean Michon; Gaelle Pierron; Heinrich Kovar; Udo Kontny; Anna González-Neira; Javier Alonso; Ana Patino-Garcia; Nadège Corradini; Perrine Marec Bérard; Jeremy Miller; Neal D Freedman; Nathaniel Rothman; Brian D Carter; Casey L Dagnall; Laurie Burdett; Kristine Jones; Michelle Manning; Kathleen Wyatt; Weiyin Zhou; Meredith Yeager; David G Cox; Robert N Hoover; Javed Khan; Gregory T Armstrong; Wendy M Leisenring; Smita Bhatia; Leslie L Robison; Andreas E Kulozik; Jennifer Kriebel; Thomas Meitinger; Markus Metzler; Manuela Krumbholz; Wolfgang Hartmann; Konstantin Strauch; Thomas Kirchner; Uta Dirksen; Lisa Mirabello; Margaret A Tucker; Franck Tirode; Lindsay M Morton; Stephen J Chanock; Olivier Delattre; Mitchell J Machiela Journal: PLoS One Date: 2020-09-03 Impact factor: 3.752