Ali Reza Abaspour1, Mohammad Taghikhani2, Seyed Mohammad Reza Parizadeh3, Seyed Mohammad Reza Seyedi4, Hamideh Ghazizadeh3, Elham Kazemi5, Mohsen Moohebati6, Fahime Ghafoori1, Maryam Mardannik7, Amir Avan8, Gordon A Ferns9, Majid Ghayour-Mobarhan10. 1. Department of Molecular Science, North Khorasan University of Medical Science, Faculty of Medicine, Bojnurd, Iran. 2. Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. 3. Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. 4. Department of Biology, Faculty of Science, Ferdowsi University of Mashhad. Mashhad, Iran. 5. Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran. 6. Cardiovascular Research Center, Avicenna Research Institute, Mashhad University of Medical Science (MUMS), Mashhad, Iran. 7. Department of Biochemistry, Payam Nor University, Faculty of Basic Science, Mashhad, Iran. 8. Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. 9. Brighton & Sussex Medical School, Division of Medical Education, Falmer, Brighton, Sussex BN1 9PH, UK. 10. Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: ghayourm@mums.ac.ir.
Abstract
BACKGROUND: Coronary artery disease (CAD), is one of the leading causes of death globally. CAD risk factors, such as smoking, dyslipidemia, and obesity, are mainly associated with increased oxidative stress. Heat Shock Protein-27 (HSP27) has a protective role in conditions of oxidative stress. The aim of the current study was to investigate the relationship between HSP27 mRNA copy numbers in the peripheral blood mononuclear cell (PBMCs) and the degree of CAD progression. METHODS: A total of 103 subjects aged 49-71 years were recruited; Patients with CAD were categorized into two groups: patients having <50% stenosis (Angio-) and ≥50% stenosis (Angio+). The mRNA copy numbers of HSP-27 in PBMCs, anthropometric-parameters, fasting blood glucose (FBG), and the fasted serum lipid profile were evaluated. RESULTS: Angio+ patients had a significantly higher level of TC and LDL-C values compared with Angio- patients and the control group (p < 0.05). The HSP27 expression in PBMCs was significantly increased in Angio+ and Angio- subjects, compared to the control group. Moreover, there was a significant association between the FBG, TC, LDL-C and TG among the groups (p < 0.05). CONCLUSION: It was shown that the increased expression of HSP27 in PBMCs of CAD patients is significantly correlated with CAD severity in Angio+ subjects, which can be used as an early prognostic biomarker, indicating the degree of overall oxidative stress in patients. In order to verify this statement, it is suggested to measure Pro-oxidant- Antioxidant Balance (PAB) test by the same design in subsequent studies.
BACKGROUND:Coronary artery disease (CAD), is one of the leading causes of death globally. CAD risk factors, such as smoking, dyslipidemia, and obesity, are mainly associated with increased oxidative stress. Heat Shock Protein-27 (HSP27) has a protective role in conditions of oxidative stress. The aim of the current study was to investigate the relationship between HSP27 mRNA copy numbers in the peripheral blood mononuclear cell (PBMCs) and the degree of CAD progression. METHODS: A total of 103 subjects aged 49-71 years were recruited; Patients with CAD were categorized into two groups: patients having <50% stenosis (Angio-) and ≥50% stenosis (Angio+). The mRNA copy numbers of HSP-27 in PBMCs, anthropometric-parameters, fasting blood glucose (FBG), and the fasted serum lipid profile were evaluated. RESULTS: Angio+ patients had a significantly higher level of TC and LDL-C values compared with Angio- patients and the control group (p < 0.05). The HSP27 expression in PBMCs was significantly increased in Angio+ and Angio- subjects, compared to the control group. Moreover, there was a significant association between the FBG, TC, LDL-C and TG among the groups (p < 0.05). CONCLUSION: It was shown that the increased expression of HSP27 in PBMCs of CAD patients is significantly correlated with CAD severity in Angio+ subjects, which can be used as an early prognostic biomarker, indicating the degree of overall oxidative stress in patients. In order to verify this statement, it is suggested to measure Pro-oxidant- Antioxidant Balance (PAB) test by the same design in subsequent studies.