Literature DB >> 31219641

New tools for recombinant protein production in Escherichia coli: A 5-year update.

Germán L Rosano1, Enrique S Morales1, Eduardo A Ceccarelli1.   

Abstract

The production of proteins in sufficient amounts is key for their study or use as biotherapeutic agents. Escherichia coli is the host of choice for recombinant protein production given its fast growth, easy manipulation, and cost-effectiveness. As such, its protein production capabilities are continuously being improved. Also, the associated tools (such as plasmids and cultivation conditions) are subject of ongoing research to optimize product yield. In this work, we review the latest advances in recombinant protein production in E. coli.
© 2019 The Protein Society.

Entities:  

Keywords:  E. coli strains; Escherichia coli; affinity tags; expression plasmid; fusion partners; inclusion bodies; recombinant protein production

Mesh：

Substances：

Year:  2019        PMID： 31219641      PMCID： PMC6635841          DOI： 10.1002/pro.3668

Source DB:  PubMed          Journal:  Protein Sci        ISSN： 0961-8368            Impact factor:   6.725


  76 in total

1.  Tuning Escherichia coli for membrane protein overexpression.

Authors:  Samuel Wagner; Mirjam M Klepsch; Susan Schlegel; Ansgar Appel; Roger Draheim; Michael Tarry; Martin Högbom; Klaas J van Wijk; Dirk J Slotboom; Jan O Persson; Jan-Willem de Gier
Journal:  Proc Natl Acad Sci U S A       Date:  2008-09-16       Impact factor: 11.205

2.  Fed-batch mode in shake flasks by slow-release technique.

Authors:  M Jeude; B Dittrich; H Niederschulte; T Anderlei; C Knocke; D Klee; J Büchs
Journal:  Biotechnol Bioeng       Date:  2006-10-20       Impact factor: 4.530

3.  Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.

Authors:  F W Studier; B A Moffatt
Journal:  J Mol Biol       Date:  1986-05-05       Impact factor: 5.469

4.  Expression and purification of recombinant proteins in Escherichia coli tagged with a small metal-binding protein from Nitrosomonas europaea.

Authors:  Teresa Vargas-Cortez; Jose Ruben Morones-Ramirez; Isaias Balderas-Renteria; Xristo Zarate
Journal:  Protein Expr Purif       Date:  2015-10-20       Impact factor: 1.650

5.  ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification.

Authors:  J Grodberg; J J Dunn
Journal:  J Bacteriol       Date:  1988-03       Impact factor: 3.490

6.  Optimization of membrane protein overexpression and purification using GFP fusions.

Authors:  David Drew; Mirjam Lerch; Edmund Kunji; Dirk-Jan Slotboom; Jan-Willem de Gier
Journal:  Nat Methods       Date:  2006-04       Impact factor: 28.547

7.  Improving the expression of recombinant proteins in E. coli BL21 (DE3) under acetate stress: an alkaline pH shift approach.

Authors:  Hengwei Wang; Fengqing Wang; Wei Wang; Xueling Yao; Dongzhi Wei; Hairong Cheng; Zixin Deng
Journal:  PLoS One       Date:  2014-11-17       Impact factor: 3.240

8.  Diverse genetic error modes constrain large-scale bio-based production.

Authors:  Peter Rugbjerg; Nils Myling-Petersen; Andreas Porse; Kira Sarup-Lytzen; Morten O A Sommer
Journal:  Nat Commun       Date:  2018-02-20       Impact factor: 14.919

Review 9.  Inclusion bodies: not that bad….

Authors:  Ana Ramón; Mario Señorale-Pose; Mónica Marín
Journal:  Front Microbiol       Date:  2014-02-14       Impact factor: 5.640

10.  Multi-Compartment and Multi-Host Vector Suite for Recombinant Protein Expression and Purification.

Authors:  Claudia Ortega; Daniel Prieto; Cecilia Abreu; Pablo Oppezzo; Agustín Correa
Journal:  Front Microbiol       Date:  2018-06-27       Impact factor: 5.640
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  42 in total

Review 1.  Stepwise optimization of recombinant protein production in Escherichia coli utilizing computational and experimental approaches.

Authors:  Kulandai Arockia Rajesh Packiam; Ramakrishnan Nagasundara Ramanan; Chien Wei Ooi; Lakshminarasimhan Krishnaswamy; Beng Ti Tey
Journal:  Appl Microbiol Biotechnol       Date:  2020-02-19       Impact factor: 4.813

2.  Structure Determination of Membrane Proteins Using X-Ray Crystallography.

Authors:  Evan Billings; Karl Lundquist; Claire Overly; Karthik Srinivasan; Nicholas Noinaj
Journal:  Methods Mol Biol       Date:  2021

Review 3.  Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development.

Authors:  Nagesh K Tripathi; Ambuj Shrivastava
Journal:  Front Bioeng Biotechnol       Date:  2019-12-20

4.  A Guideline to Set Up Cascaded Continuous Cultivation with E. coli Bl21 (DE3).

Authors:  Julian Kopp; Oliver Spadiut
Journal:  Methods Mol Biol       Date:  2022

5.  Recombinant Protein Production and Purification of Insoluble Proteins.

Authors:  Neus Ferrer-Miralles; Paolo Saccardo; José Luis Corchero; Elena Garcia-Fruitós
Journal:  Methods Mol Biol       Date:  2022

6.  A Robust Platform for Unnatural Amino Acid Mutagenesis in E. coli Using the Bacterial Tryptophanyl-tRNA synthetase/tRNA pair.

Authors:  Elise D Ficaretta; Chester J J Wrobel; Soumya J S Roy; Sarah B Erickson; James S Italia; Abhishek Chatterjee
Journal:  J Mol Biol       Date:  2021-10-13       Impact factor: 5.469

7.  Cloning, expression and characterization of PURase gene from Pseudomonas sp. AKS31.

Authors:  Mousumi Saha; Shankari Prasad Dutta; Goutam Mukherjee; Aparajita Basu; Debarati Majumder; Alok Kumar Sil
Journal:  Arch Microbiol       Date:  2022-07-18       Impact factor: 2.667

Review 8.  Current trends in biopharmaceuticals production in Escherichia coli.

Authors:  L McElwain; K Phair; C Kealey; D Brady
Journal:  Biotechnol Lett       Date:  2022-07-07       Impact factor: 2.716

9.  Expression of Bacillus amyloliquefaciens transglutaminase in recombinant E. coli under the control of a bicistronic plasmid system in DO-stat fed-batch bioreactor cultivations.

Authors:  Lovaine Silva Duarte; Carla Roberta Matte; Paulo Roberto Dall Cortivo; José Eduardo Sacconi Nunes; Laisa Quadros Barsé; Cristiano Valim Bizarro; Marco Antônio Záchia Ayub
Journal:  Braz J Microbiol       Date:  2021-05-19       Impact factor: 2.476

10.  TISIGNER.com: web services for improving recombinant protein production.

Authors:  Bikash K Bhandari; Chun Shen Lim; Paul P Gardner
Journal:  Nucleic Acids Res       Date:  2021-07-02       Impact factor: 16.971
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