Literature DB >> 16554836

Optimization of membrane protein overexpression and purification using GFP fusions.

David Drew1, Mirjam Lerch, Edmund Kunji, Dirk-Jan Slotboom, Jan-Willem de Gier.   

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Year:  2006        PMID: 16554836     DOI: 10.1038/nmeth0406-303

Source DB:  PubMed          Journal:  Nat Methods        ISSN: 1548-7091            Impact factor:   28.547


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  128 in total

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4.  A green fluorescent protein screen for identification of well-expressed membrane proteins from a cohort of extremophilic organisms.

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Journal:  Protein Sci       Date:  2009-01       Impact factor: 6.725

5.  Fluorescent probe for high-throughput screening of membrane protein expression.

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Journal:  Protein Sci       Date:  2013-07-03       Impact factor: 6.725

6.  Functional characterization of SbmA, a bacterial inner membrane transporter required for importing the antimicrobial peptide Bac7(1-35).

Authors:  Giulia Runti; Maria del Carmen Lopez Ruiz; Tatiana Stoilova; Rohanah Hussain; Matthew Jennions; Hassanul G Choudhury; Monica Benincasa; Renato Gennaro; Konstantinos Beis; Marco Scocchi
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7.  Membrane protein stability can be compromised by detergent interactions with the extramembranous soluble domains.

Authors:  Zhengrong Yang; Chi Wang; Qingxian Zhou; Jianli An; Ellen Hildebrandt; Luba A Aleksandrov; John C Kappes; Lawrence J DeLucas; John R Riordan; Ina L Urbatsch; John F Hunt; Christie G Brouillette
Journal:  Protein Sci       Date:  2014-05-03       Impact factor: 6.725

8.  Green Fluorescent Protein as a protein localization and topological reporter in mycobacteria.

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Journal:  Tuberculosis (Edinb)       Date:  2017-04-10       Impact factor: 3.131

9.  An Improved Strategy for Fluorescent Tagging of Membrane Proteins for Overexpression and Purification in Mammalian Cells.

Authors:  Mitra S Rana; Xiyu Wang; Anirban Banerjee
Journal:  Biochemistry       Date:  2018-11-27       Impact factor: 3.162

10.  GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae.

Authors:  David Drew; Simon Newstead; Yo Sonoda; Hyun Kim; Gunnar von Heijne; So Iwata
Journal:  Nat Protoc       Date:  2008       Impact factor: 13.491

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