| Literature DB >> 31216469 |
Bo Wang1, Linlin Wu2, Dongwei Li1, Yuting Liu1, Jing Guo3, Chen Li4, Yuxiang Yao5, Yaofeng Wang1, Guoqing Zhao6, Xiaoshan Wang1, Meijun Fu4, He Liu1, Shangtao Cao1, Chuman Wu1, Shengyong Yu4, Chunhua Zhou4, Yue Qin4, Junqi Kuang4, Jin Ming4, Shilong Chu1, Xuejie Yang1, Ping Zhu7, Guangjin Pan1, Jiekai Chen1, Jing Liu8, Duanqing Pei9.
Abstract
Reprogramming somatic cells to pluripotency by Oct4, Sox2, Klf4, and Myc represent a paradigm for cell fate determination. Here, we report a combination of Jdp2, Jhdm1b, Mkk6, Glis1, Nanog, Essrb, and Sall4 (7F) that reprogram mouse embryonic fibroblasts or MEFs to chimera competent induced pluripotent stem cells (iPSCs) efficiently. RNA sequencing (RNA-seq) and ATAC-seq reveal distinct mechanisms for 7F induction of pluripotency. Dropout experiments further reveal a highly cooperative process among 7F to dynamically close and open chromatin loci that encode a network of transcription factors to mediate reprogramming. These results establish an alternative paradigm for reprogramming that may be useful for analyzing cell fate control.Entities:
Year: 2019 PMID： 31216469 DOI： 10.1016/j.celrep.2019.05.068
Source DB: PubMed Journal: Cell Rep Impact factor: 9.423