Literature DB >> 3118358

Identification of a DNA binding protein that recognizes the nonamer recombinational signal sequence of immunoglobulin genes.

B D Halligan1, S V Desiderio.   

Abstract

Extracts of nuclei from B- and T-lymphoid cells contain a protein that binds specifically to the conserved nonamer DNA sequence within the recombinational signals of immunoglobulin genes. Complexes with DNA fragments from four kappa light-chain joining (J) segments have the same electrophoretic mobility. Nonamer-containing DNA fragments from heavy-chain and light-chain genes compete for binding. Within the 5'-flanking DNA of the J kappa 4 gene segment, the binding site has been localized to a 27-base-pair interval spanning the nonamer region. The binding activity is recovered as a single peak after ion-exchange chromatography. The site of binding of the protein and its presence in nuclei of lymphoid cells suggest that it may function in the assembly of immunoglobulin genes.

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Year:  1987        PMID： 3118358      PMCID： PMC299220          DOI： 10.1073/pnas.84.20.7019

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  29 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  Continuing rearrangement of immunoglobulin and T-cell receptor genes in a Ha-ras-transformed lymphoid progenitor cell line.

Authors:  A Alessandrini; J H Pierce; D Baltimore; S V Desiderio
Journal:  Proc Natl Acad Sci U S A       Date:  1987-04       Impact factor: 11.205

3.  Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.

Authors:  M Fried; D M Crothers
Journal:  Nucleic Acids Res       Date:  1981-12-11       Impact factor: 16.971

4.  Continuing kappa-gene rearrangement in a cell line transformed by Abelson murine leukemia virus.

Authors:  S Lewis; N Rosenberg; F Alt; D Baltimore
Journal:  Cell       Date:  1982-10       Impact factor: 41.582

5.  Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions.

Authors:  F W Alt; D Baltimore
Journal:  Proc Natl Acad Sci U S A       Date:  1982-07       Impact factor: 11.205

Review 6.  Somatic generation of antibody diversity.

Authors:  S Tonegawa
Journal:  Nature       Date:  1983-04-14       Impact factor: 49.962

7.  Identification of D segments of immunoglobulin heavy-chain genes and their rearrangement in T lymphocytes.

Authors:  Y Kurosawa; H von Boehmer; W Haas; H Sakano; A Trauneker; S Tonegawa
Journal:  Nature       Date:  1981-04-16       Impact factor: 49.962

8.  An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH.

Authors:  P Early; H Huang; M Davis; K Calame; L Hood
Journal:  Cell       Date:  1980-04       Impact factor: 41.582

9.  A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.

Authors:  M M Garner; A Revzin
Journal:  Nucleic Acids Res       Date:  1981-07-10       Impact factor: 16.971

10.  Somatic rearrangements forming active immunoglobulin mu genes in B and T lymphoid cell lines.

Authors:  S Cory; J M Adams; D J Kemp
Journal:  Proc Natl Acad Sci U S A       Date:  1980-08       Impact factor: 11.205
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  10 in total

1.  Deletion of human JK segments by site-specific recombination recognizing the conserved nonamer and heptamer sequences.

Authors:  T Nakatani; K Horigome; N Nomura; T Kondo; H Ohtsuka; H Noguchi; T Honjo
Journal:  Nucleic Acids Res       Date:  1990-09-25       Impact factor: 16.971

2.  HMG1-related DNA-binding protein isolated with V-(D)-J recombination signal probes.

Authors:  M Shirakata; K Hüppi; S Usuda; K Okazaki; K Yoshida; H Sakano
Journal:  Mol Cell Biol       Date:  1991-09       Impact factor: 4.272

3.  Methylated DNA-binding protein is present in various mammalian cell types.

Authors:  P C Supakar; D Weist; D L Zhang; N Inamdar; X Y Zhang; R Khan; K C Ehrlich; M Ehrlich
Journal:  Nucleic Acids Res       Date:  1988-08-25       Impact factor: 16.971

4.  Purification and characterization of a protein that binds to the recombination signal sequence of the immunoglobulin J kappa segment.

Authors:  Y Hamaguchi; N Matsunami; Y Yamamoto; T Honjo
Journal:  Nucleic Acids Res       Date:  1989-11-25       Impact factor: 16.971

Review 5.  Nobel lecture in physiology or medicine--1987. Somatic generation of immune diversity.

Authors:  S Tonegawa
Journal:  In Vitro Cell Dev Biol       Date:  1988-04

6.  rag-1 and rag-2 are components of a high-molecular-weight complex, and association of rag-2 with this complex is rag-1 dependent.

Authors:  T M Leu; D G Schatz
Journal:  Mol Cell Biol       Date:  1995-10       Impact factor: 4.272

7.  Recombination sequence-binding protein in thymocytes undergoing T-cell receptor gene rearrangement.

Authors:  K Muegge; M West; S K Durum
Journal:  Proc Natl Acad Sci U S A       Date:  1993-05-01       Impact factor: 11.205

8.  RAG-1 and RAG-2 are not sufficient to direct all phases of immunoglobulin gene rearrangement in pre-B-cell lines.

Authors:  L C Wang; N Rosenberg
Journal:  Mol Cell Biol       Date:  1993-07       Impact factor: 4.272

Review 9.  The SCID mouse: relevance as an animal model system for studying human disease.

Authors:  E A Hendrickson
Journal:  Am J Pathol       Date:  1993-12       Impact factor: 4.307

10.  Purification of core-binding factor, a protein that binds the conserved core site in murine leukemia virus enhancers.

Authors:  S W Wang; N A Speck
Journal:  Mol Cell Biol       Date:  1992-01       Impact factor: 4.272
  10 in total

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