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Literature DB >> 2120678

Deletion of human JK segments by site-specific recombination recognizing the conserved nonamer and heptamer sequences.

T Nakatani¹, K Horigome, N Nomura, T Kondo, H Ohtsuka, H Noguchi, T Honjo.

Abstract

Mapping and partial sequencing of the productive K chain genomic DNA of FK-001 demonstrated a 1.8-kb deletion including the JK2, JK3, JK4, and JK5 segments. This deletion occurred between the heptamer recombination signal sequence of the JK2 segment and the heptamer-like sequence located 1.8 kb downstream of the JK2 segment. The recombination reaction kept the reciprocally joined signal sequences on the chromosome and deleted the intervening DNA segment. The cloned FK-001 K chain gene was expressed efficiently in mouse myeloma cells, demonstrating that the 1.8-kb deleted region conferred no functions for gene expression.

Entities: Disease Species

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Year: 1990 PMID： 2120678 PMCID： PMC332233 DOI： 10.1093/nar/18.18.5529

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

17 in total

1. High-frequency transfer of cloned herpes simplex virus type 1 sequences to mammalian cells by protoplast fusion.

Authors: R M Sandri-Goldin; A L Goldin; M Levine; J C Glorioso
Journal: Mol Cell Biol Date: 1981-08 Impact factor: 4.272

2. Isolation of an excision product of T-cell receptor alpha-chain gene rearrangements.

Authors: S Fujimoto; H Yamagishi
Journal: Nature Date: 1987 May 21-27 Impact factor: 49.962

3. The human CK gene segment and the kappa deleting element are closely linked.

Authors: H G Klobeck; H G Zachau
Journal: Nucleic Acids Res Date: 1986-06-11 Impact factor: 16.971

4. T cell receptor beta gene sequences in the circular DNA of thymocyte nuclei: direct evidence for intramolecular DNA deletion in V-D-J joining.

Authors: K Okazaki; D D Davis; H Sakano
Journal: Cell Date: 1987-05-22 Impact factor: 41.582

5. Nonproductive kappa immunoglobulin genes: recombinational abnormalities and other lesions affecting transcription, RNA processing, turnover, and translation.

Authors: D E Kelley; L M Wiedemann; A C Pittet; S Strauss; K J Nelson; J Davis; B Van Ness; R P Perry
Journal: Mol Cell Biol Date: 1985-07 Impact factor: 4.272

2. Characterization of a preferred site on human chromosome 19q for integration of adeno-associated virus DNA by non-homologous recombination.

Authors: R M Kotin; R M Linden; K I Berns
Journal: EMBO J Date: 1992-12 Impact factor: 11.598

2 in total

Deletion of human JK segments by site-specific recombination recognizing the conserved nonamer and heptamer sequences.

1. High-frequency transfer of cloned herpes simplex virus type 1 sequences to mammalian cells by protoplast fusion.

2. Isolation of an excision product of T-cell receptor alpha-chain gene rearrangements.

3. The human CK gene segment and the kappa deleting element are closely linked.

4. T cell receptor beta gene sequences in the circular DNA of thymocyte nuclei: direct evidence for intramolecular DNA deletion in V-D-J joining.

5. Nonproductive kappa immunoglobulin genes: recombinational abnormalities and other lesions affecting transcription, RNA processing, turnover, and translation.

6. Dideoxy sequencing method using denatured plasmid templates.

7. A mutant immunoglobulin light chain is formed by aberrant DNA- and RNA-splicing events.

8. An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH.

9. A uniform deleting element mediates the loss of kappa genes in human B cells.

10. Human immunoglobulin kappa light-chain genes are deleted or rearranged in lambda-producing B cells.

1. Cryptic signals and the fidelity of V(D)J joining.

2. Characterization of a preferred site on human chromosome 19q for integration of adeno-associated virus DNA by non-homologous recombination.