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Literature DB >> 31178255

Harnessing Hematopoietic Stem Cell Low Intracellular Calcium Improves Their Maintenance In Vitro.

Larry L Luchsinger¹, Alexandros Strikoudis², Nichole M Danzl³, Erin C Bush⁴, Michael O Finlayson⁴, Prakash Satwani⁵, Megan Sykes⁶, Masayuki Yazawa⁷, Hans-Willem Snoeck⁸.

Abstract

The specific cellular physiology of hematopoietic stem cells (HSCs) is underexplored, and their maintenance in vitro remains challenging. We discovered that culture of HSCs in low calcium increased their maintenance as determined by phenotype, function, and single-cell expression signature. HSCs are endowed with low intracellular calcium conveyed by elevated activity of glycolysis-fueled plasma membrane calcium efflux pumps and a low-bone-marrow interstitial fluid calcium concentration. Low-calcium conditions inhibited calpain proteases, which target ten-eleven translocated (TET) enzymes, of which TET2 was required for the effect of low calcium conditions on HSC maintenance in vitro. These observations reveal a physiological feature of HSCs that can be harnessed to improve their maintenance in vitro.

Entities: CellLine Chemical Disease Gene Mutation Species

Keywords: TET enzymes; calcium; calpains; culture; hematopoiesis; hematopoietic stem cells

Mesh：

Substances：

Year: 2019 PMID： 31178255 PMCID： PMC7649753 DOI： 10.1016/j.stem.2019.05.002

Source DB: PubMed Journal: Cell Stem Cell ISSN： 1875-9777 Impact factor: 24.633

92 in total

1. Bcrp1 gene expression is required for normal numbers of side population stem cells in mice, and confers relative protection to mitoxantrone in hematopoietic cells in vivo.

Authors: Sheng Zhou; John J Morris; Yuxiao Barnes; Lubin Lan; John D Schuetz; Brian P Sorrentino
Journal: Proc Natl Acad Sci U S A Date: 2002-09-06 Impact factor: 11.205

2. Microelectrode studies on the acid microenvironment beneath adherent macrophages and osteoclasts.

Authors: I A Silver; R J Murrills; D J Etherington
Journal: Exp Cell Res Date: 1988-04 Impact factor: 3.905

3. Quantitative imaging of haematopoietic stem and progenitor cell localization and hypoxic status in the bone marrow microenvironment.

Authors: César Nombela-Arrieta; Gregory Pivarnik; Beatrice Winkel; Kimberly J Canty; Brendan Harley; John E Mahoney; Shin-Young Park; Jiayun Lu; Alexei Protopopov; Leslie E Silberstein
Journal: Nat Cell Biol Date: 2013-04-28 Impact factor: 28.824

4. TET1 is a tumor suppressor of hematopoietic malignancy.

Authors: Luisa Cimmino; Meelad M Dawlaty; Delphine Ndiaye-Lobry; Yoon Sing Yap; Sofia Bakogianni; Yiting Yu; Sanchari Bhattacharyya; Rita Shaknovich; Huimin Geng; Camille Lobry; Jasper Mullenders; Bryan King; Thomas Trimarchi; Beatriz Aranda-Orgilles; Cynthia Liu; Steven Shen; Amit K Verma; Rudolf Jaenisch; Iannis Aifantis
Journal: Nat Immunol Date: 2015-04-13 Impact factor: 25.606

5. Stem cell aging. A mitochondrial UPR-mediated metabolic checkpoint regulates hematopoietic stem cell aging.

Authors: Mary Mohrin; Jiyung Shin; Yufei Liu; Katharine Brown; Hanzhi Luo; Yannan Xi; Cole M Haynes; Danica Chen
Journal: Science Date: 2015-03-20 Impact factor: 47.728

6. Conversion of adult endothelium to immunocompetent haematopoietic stem cells.

Authors: Raphael Lis; Charles C Karrasch; Michael G Poulos; Balvir Kunar; David Redmond; Jose G Barcia Duran; Chaitanya R Badwe; William Schachterle; Michael Ginsberg; Jenny Xiang; Arash Rafii Tabrizi; Koji Shido; Zev Rosenwaks; Olivier Elemento; Nancy A Speck; Jason M Butler; Joseph M Scandura; Shahin Rafii
Journal: Nature Date: 2017-05-17 Impact factor: 49.962

7. Mitofusin 2 tethers endoplasmic reticulum to mitochondria.

Authors: Olga Martins de Brito; Luca Scorrano
Journal: Nature Date: 2008-12-04 Impact factor: 49.962

8. Expression of retroviral vectors containing the human multidrug resistance 1 cDNA in hematopoietic cells of transplanted mice.

Authors: B P Sorrentino; K T McDonagh; D Woods; D Orlic
Journal: Blood Date: 1995-07-15 Impact factor: 22.113

9. Ascorbate regulates haematopoietic stem cell function and leukaemogenesis.

Authors: Michalis Agathocleous; Corbin E Meacham; Rebecca J Burgess; Elena Piskounova; Zhiyu Zhao; Genevieve M Crane; Brianna L Cowin; Emily Bruner; Malea M Murphy; Weina Chen; Gerald J Spangrude; Zeping Hu; Ralph J DeBerardinis; Sean J Morrison
Journal: Nature Date: 2017-08-21 Impact factor: 49.962

10. Molecular signatures of proliferation and quiescence in hematopoietic stem cells.

Authors: Teresa A Venezia; Akil A Merchant; Carlos A Ramos; Nathan L Whitehouse; Andrew S Young; Chad A Shaw; Margaret A Goodell
Journal: PLoS Biol Date: 2004-09-28 Impact factor: 8.029

13 in total

Review 1. Stabilizing hematopoietic stem cells in vitro.

Authors: Adam C Wilkinson; Hiromitsu Nakauchi
Journal: Curr Opin Genet Dev Date: 2020-06-20 Impact factor: 5.578

2. Induction of functional neutrophils from mouse fibroblasts by thymidine through enhancement of Tet3 activity.

Authors: Buqing Ye; Liuliu Yang; Benyu Liu; Nian Liu; Dongdong Fan; Huimu Li; Lei Sun; Ying Du; Shuo Wang; Yong Tian; Zusen Fan
Journal: Cell Mol Immunol Date: 2022-03-17 Impact factor: 22.096

Review 3. Haematopoietic stem cell self-renewal in vivo and ex vivo.

Authors: Adam C Wilkinson; Kyomi J Igarashi; Hiromitsu Nakauchi
Journal: Nat Rev Genet Date: 2020-05-28 Impact factor: 53.242

Review 4. Hematopoietic Stem Cell Metabolism during Development and Aging.

Authors: Ayako Nakamura-Ishizu; Keisuke Ito; Toshio Suda
Journal: Dev Cell Date: 2020-07-20 Impact factor: 12.270

Review 5. Calcium regulation of stem cells.

Authors: Hans-Willem Snoeck
Journal: EMBO Rep Date: 2020-05-17 Impact factor: 8.807

6. S100A6 is a critical regulator of hematopoietic stem cells.

Authors: Tan Hooi Min Grahn; Abhishek Niroula; Ákos Végvári; Leal Oburoglu; Maroulio Pertesi; Sarah Warsi; Fatemeh Safi; Natsumi Miharada; Sandra C Garcia; Kavitha Siva; Yang Liu; Emma Rörby; Björn Nilsson; Roman A Zubarev; Stefan Karlsson
Journal: Leukemia Date: 2020-06-19 Impact factor: 11.528

Harnessing Hematopoietic Stem Cell Low Intracellular Calcium Improves Their Maintenance In Vitro.

1. Bcrp1 gene expression is required for normal numbers of side population stem cells in mice, and confers relative protection to mitoxantrone in hematopoietic cells in vivo.

2. Microelectrode studies on the acid microenvironment beneath adherent macrophages and osteoclasts.

3. Quantitative imaging of haematopoietic stem and progenitor cell localization and hypoxic status in the bone marrow microenvironment.

4. TET1 is a tumor suppressor of hematopoietic malignancy.

5. Stem cell aging. A mitochondrial UPR-mediated metabolic checkpoint regulates hematopoietic stem cell aging.

6. Conversion of adult endothelium to immunocompetent haematopoietic stem cells.

7. Mitofusin 2 tethers endoplasmic reticulum to mitochondria.

8. Expression of retroviral vectors containing the human multidrug resistance 1 cDNA in hematopoietic cells of transplanted mice.

9. Ascorbate regulates haematopoietic stem cell function and leukaemogenesis.

10. Molecular signatures of proliferation and quiescence in hematopoietic stem cells.

Review 1. Stabilizing hematopoietic stem cells in vitro.

2. Induction of functional neutrophils from mouse fibroblasts by thymidine through enhancement of Tet3 activity.

Review 3. Haematopoietic stem cell self-renewal in vivo and ex vivo.

Review 4. Hematopoietic Stem Cell Metabolism during Development and Aging.

Review 5. Calcium regulation of stem cells.

6. S100A6 is a critical regulator of hematopoietic stem cells.

7. Engineering of a bona fide light-operated calcium channel.

Review 8. Put in a "Ca²⁺ll" to Acute Myeloid Leukemia.

9. Dysfunction of metabolic activity of bone marrow mesenchymal stem cells in aged mice.

10. Quantification of bone marrow interstitial pH and calcium concentration by intravital ratiometric imaging.

Review 1. Stabilizing hematopoietic stem cells in vitro.

Review 3. Haematopoietic stem cell self-renewal in vivo and ex vivo.

Review 4. Hematopoietic Stem Cell Metabolism during Development and Aging.

Review 5. Calcium regulation of stem cells.

Review 8. Put in a "Ca2+ll" to Acute Myeloid Leukemia.

Review 8. Put in a "Ca²⁺ll" to Acute Myeloid Leukemia.