Wenhua Zang1,2, Hua Bian3,2, Xianzhang Huang1,2, Gang Yin4, Chaoyun Zhang1,2, L I Han1,2, Pengfei Hao1,2, Shengchen Ding1,2, Y U Sun5, Zhijian Yang5,6, Robert M Hoffman6,7, Decai Tang8. 1. Zhang Zhongjing College of Traditional Chinese Medicine, Nanyang Institute of Technology, Nanyang, P.R. China. 2. Henan Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation, Nanyang, P.R. China. 3. Zhang Zhongjing College of Traditional Chinese Medicine, Nanyang Institute of Technology, Nanyang, P.R. China biancrown@163.com talknow@163.com. 4. Nanjing University of Chinese Medicine, Nanjing, P.R. China. 5. Origin Biosciences Inc., Nanjing, P.R. China. 6. AntiCancer, Inc., San Diego, CA, U.S.A. 7. Department of Surgery, University of California, San Diego, CA, U.S.A. 8. Nanjing University of Chinese Medicine, Nanjing, P.R. China biancrown@163.com talknow@163.com.
Abstract
BACKGROUND/AIM: The aim of the present study was to investigate the vascular normalization effect of traditional Chinese medicine Astragalus membranaceus (AM) and Curcuma wenyujin (CW) on tumor-derived endothelial cells (TECs). MATERIALS AND METHODS: TECs were isolated from the xenografted HCC cell line HepG2 expressing red fluorescent protein (RFP). The effect of AM and CW on TECs proliferation was measured using the CCK8 assay. The vascular normalization potential of AM and CW was assessed using a tube formation assay. Immunocytochemistry was performed to assess the effect of AM and CW on the expression of angiogenic maker CD34 and hypoxia-inducible factor HIF1a. RESULTS: The isolated TECs and endothelioma (EOMA) cells did not differ with regard to the expression levels of endothelial markers CD34, VEGFR-1, VEGFR-2, PDGFR-α and PDGFR-β. All AM, CW, AM+CW and Nintedanib (Nin) showed a dose-dependent increasing inhibition effect on either TECs or EOMA cells. AM, CW and AM+CW significantly reduced HIF1a expression, increased CD34 expression and enhanced endothelial network formation in TECs or EOMA cells compared to the control. CONCLUSION: AM and CW promoted vascular normalization in tumor-derived endothelial cells of HCC, through increased expression of CD34 and reduced expression of HIF1a. Copyright
BACKGROUND/AIM: The aim of the present study was to investigate the vascular normalization effect of traditional Chinese medicine Astragalus membranaceus (AM) and Curcuma wenyujin (CW) on tumor-derived endothelial cells (TECs). MATERIALS AND METHODS: TECs were isolated from the xenografted HCC cell line HepG2 expressing red fluorescent protein (RFP). The effect of AM and CW on TECs proliferation was measured using the CCK8 assay. The vascular normalization potential of AM and CW was assessed using a tube formation assay. Immunocytochemistry was performed to assess the effect of AM and CW on the expression of angiogenic maker CD34 and hypoxia-inducible factor HIF1a. RESULTS: The isolated TECs and endothelioma (EOMA) cells did not differ with regard to the expression levels of endothelial markers CD34, VEGFR-1, VEGFR-2, PDGFR-α and PDGFR-β. All AM, CW, AM+CW and Nintedanib (Nin) showed a dose-dependent increasing inhibition effect on either TECs or EOMA cells. AM, CW and AM+CW significantly reduced HIF1a expression, increased CD34 expression and enhanced endothelial network formation in TECs or EOMA cells compared to the control. CONCLUSION:AM and CW promoted vascular normalization in tumor-derived endothelial cells of HCC, through increased expression of CD34 and reduced expression of HIF1a. Copyright
Authors: Hiroto Nishino; Hannah M Hollandsworth; Norihiko Sugisawa; Jun Yamamoto; Yoshihiko Tashiro; Sachiko Inubushi; Kazuyuki Hamada; Y U Sun; Hyein Lim; Siamak Amirfakhri; Filemoni Filemoni; Robert M Hoffman; Michael Bouvet Journal: In Vivo Date: 2020 Nov-Dec Impact factor: 2.155