Juan Zhang1, Hong Chen2, Yuancheng Bao1, Daojun Xie1, Wenming Yang1, Huaizhou Jiang3, Ting Dong1, Hui Han1. 1. Encephalopathy Center, The First Affiliated Hospital of Anhui University of Chinese Medicine, 117 Meishan Road, Shushan District, Hefei 230031, China. 2. Graduate School, Anhui University of Chinese Medicine, 103 Meishan Road, Shushan District, Hefei 230038, China. 3. Basic Department of Traditional Chinese Medicine, Anhui University of Chinese Medicine, 103 Meishan Road, Shushan District, Hefei 230038, China.
Abstract
RESULTS: Firstly, 324 active compounds have been identified in the GDL formula. Meanwhile, we identified 1496 human genes which are related to WD or liver cirrhosis. Functional and pathway enrichment analysis indicated that NOD-like receptor signaling pathway, bile secretion, calcium signaling pathway, steroid hormone biosynthesis, T cell receptor signaling pathway, apoptosis, MAPK signaling pathway, and so forth can be obviously regulated by GDL. Further, in a mouse model of WD, in vivo experiments showed that GDL treatment can not only reduce the pathological symptoms of the liver but also reduce the apoptosis of hepatocytes. CONCLUSIONS: In this study, systemic pharmacological methods were proposed and the mechanism of GDL combined therapy for WD was explored. This method can be used as a reference for the study of other mechanisms of traditional Chinese medicine.
RESULTS: Firstly, 324 active compounds have been identified in the GDL formula. Meanwhile, we identified 1496 human genes which are related to WD or liver cirrhosis. Functional and pathway enrichment analysis indicated that NOD-like receptor signaling pathway, bile secretion, calcium signaling pathway, steroid hormone biosynthesis, T cell receptor signaling pathway, apoptosis, MAPK signaling pathway, and so forth can be obviously regulated by GDL. Further, in a mouse model of WD, in vivo experiments showed that GDL treatment can not only reduce the pathological symptoms of the liver but also reduce the apoptosis of hepatocytes. CONCLUSIONS: In this study, systemic pharmacological methods were proposed and the mechanism of GDL combined therapy for WD was explored. This method can be used as a reference for the study of other mechanisms of traditional Chinese medicine.
Authors: Valentina Medici; Noreene M Shibata; Kusum K Kharbanda; Janine M LaSalle; Rima Woods; Sarah Liu; Jesse A Engelberg; Sridevi Devaraj; Natalie J Török; Joy X Jiang; Peter J Havel; Bo Lönnerdal; Kyoungmi Kim; Charles H Halsted Journal: Hepatology Date: 2013-01-10 Impact factor: 17.425
Authors: Valentina Medici; Noreene M Shibata; Kusum K Kharbanda; Mohammad S Islam; Carl L Keen; Kyoungmi Kim; Brittany Tillman; Samuel W French; Charles H Halsted; Janine M LaSalle Journal: Epigenetics Date: 2013-11-12 Impact factor: 4.528