Tongbao Feng1, Ping Zhang2, Yingxin Sun2, Yan Wang2, Jichun Tong3, Hong Dai4, Zichun Hua5. 1. The State Key Laboratory of Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing, PR China; Department of Clinical Laboratory, the Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, PR China; Department of General Surgery, the Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, PR China. 2. Department of Clinical Laboratory, the Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, PR China. 3. Department of Cardiac Surgery, the Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, PR China. 4. Department of General Surgery, the Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, PR China. Electronic address: ycfqftb@hotmail.com. 5. The State Key Laboratory of Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing, PR China; Changzhou High-Tech Research Institute of Nanjing University and Jiangsu Target Pharma Laboratories Inc., Changzhou, PR China. Electronic address: zchua@nju.edu.cn.
Abstract
OBJECTIVE: Increasing evidence indicated that cancer-secreted exosomes played an important role in tumor metastasis. However, the function of exosomes in breast cancer pulmonary metastasis remains unknown. The aim of the study was to investigate the role of exosome-derived from breast cancer-secreted long non-coding RNAs (LncRNAs) on pre-metastatic niche formation in pulmonary metastasis. METHODS: Exosomes-derived from breast cancer were separated by ultracentrifugation. The high-throughput sequencing, Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway were used to detect and evaluate the differential expression of LncRNAs in lung fibroblasts with exosomes treated. And quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify candidate LncRNAs expression. RESULTS: We found that exosomes-derived from breast cancer induced lung fibroblasts proliferation and migration. In addition, a large number of LncRNAs expression abnormalities were involved in the breast cancer lung metastasis microenvironment. CONCLUSION: Our findings suggested that exosomal LncRNAs facilitated tumor pre-metastatic niche formation and represented a novel mechanistic insight into the molecular mechanism of cancer metastasis microenvironment.
OBJECTIVE: Increasing evidence indicated that cancer-secreted exosomes played an important role in tumor metastasis. However, the function of exosomes in breast cancer pulmonary metastasis remains unknown. The aim of the study was to investigate the role of exosome-derived from breast cancer-secreted long non-coding RNAs (LncRNAs) on pre-metastatic niche formation in pulmonary metastasis. METHODS: Exosomes-derived from breast cancer were separated by ultracentrifugation. The high-throughput sequencing, Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway were used to detect and evaluate the differential expression of LncRNAs in lung fibroblasts with exosomes treated. And quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify candidate LncRNAs expression. RESULTS: We found that exosomes-derived from breast cancer induced lung fibroblasts proliferation and migration. In addition, a large number of LncRNAs expression abnormalities were involved in the breast cancer lung metastasis microenvironment. CONCLUSION: Our findings suggested that exosomal LncRNAs facilitated tumor pre-metastatic niche formation and represented a novel mechanistic insight into the molecular mechanism of cancer metastasis microenvironment.
Authors: Ohanes Ashekyan; Samira Abdallah; Ayman Al Shoukari; Ghada Chamandi; Hayat Choubassy; Abdul Rahman S Itani; Nisreen Alwan; Rihab Nasr Journal: Int J Mol Sci Date: 2022-07-28 Impact factor: 6.208