| Literature DB >> 31167843 |
Satheesh Nair1, Vineet Patel2,3, Tadgh Hickey2,3, Clare Maguire2, David R Greig2, Winnie Lee2, Gauri Godbole2, Kathie Grant2, Marie Anne Chattaway2.
Abstract
Rapid and accurate differentiation of Salmonella spp. causing enteric fever from nontyphoidal Salmonella is essential for clinical management of cases, laboratory risk management, and implementation of public health measures. Current methods used for confirmation of identification, including biochemistry and serotyping as well as whole-genome sequencing analyses, take several days. Here we report the development and evaluation of a real-time PCR assay that can be performed directly on crude DNA extracts from bacterial colonies for the rapid identification of typhoidal and nontyphoidal Salmonella. © Crown copyright 2019.Entities:
Keywords: Nontyphoidal salmonellae; Salmonellazzm321990; real-time PCR; typhoidal salmonellae; whole-genome sequencing
Year: 2019 PMID: 31167843 PMCID: PMC6663909 DOI: 10.1128/JCM.00167-19
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948