Literature DB >> 31143937

MSCs inhibits the angiogenesis of HUVECs through the miR-211/Prox1 pathway.

Jian Pan1, Xianglong Wang1, Dequan Li2, Jianmin Li3, Zipei Jiang1.   

Abstract

The aim of this study was to investigate the effect of mesenchymal stem cells (MSCs) on the angiogenesis of human umbilical vein endothelial cells (HUVECs). MSCs were subconjunctival injected into rat corneal alkali burn models. Their impacts on the degree of corneal neovascularization (CNV) and corneal opacity were evaluated at 3, 6, 9 and 12 days after injection. An in vitro experiment of MSCs affecting HUVECs angiogenesis was performed and evaluated using the tube formation assay. The results showed that both CNV and corneal opacity were decreased in rats after MSCs injection. In HUVECs, angiogenesis of cells was inhibited by miR-211 overexpression. miR-211 negatively regulated Prox1 expression. Knockdown of miR-211 blocked the decrease of Prox1 expression induced by MSCs and the inhibitory effect of MSCs on the angiogenesis of HUVECs. The critical role of miR-211 in MSCs inhibition of corneal angiogenesis was confirmed in rat experiments. We concluded that MSCs inhibited the angiogenesis of HUVEC through miR-211 mediating the down-regulation of Prox1.
© The Author(s) 2019. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.

Entities:  

Keywords:  HUVECs; MSCs; Prox1; corneal angiogenesis; miR-211

Mesh：

Substances：

Year:  2019        PMID： 31143937     DOI： 10.1093/jb/mvz038

Source DB:  PubMed          Journal:  J Biochem        ISSN： 0021-924X            Impact factor:   3.387


  7 in total

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Authors:  Jian Pan; Xu Luo; Shujue Zhao; Jianmin Li; Zipei Jiang
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Review 4.  Subconjunctival injection of mesenchymal stem cells for corneal failure due to limbal stem cell deficiency: state of the art.

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Review 5.  Mesenchymal stem cells and cancer therapy: insights into targeting the tumour vasculature.

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6.  Umbilical cord miRNAs to predict neonatal early onset sepsis.

Authors:  Linda M Ernst; Leena B Mithal; Karen Mestan; Vivien Wang; Kathy A Mangold; Alexa Freedman; Sanchita Das
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7.  Pro-Angiogenic Effects of Essential Oil from Perilla frutescens and Its Main Component (Perillaldehyde) on Zebrafish Embryos and Human Umbilical Vein Endothelial Cells.

Authors:  Fei Zhou; Ou Dai; Cheng Peng; Liang Xiong; Hui Ao; Fei Liu; Qin-Mei Zhou
Journal:  Drug Des Devel Ther       Date:  2021-12-11       Impact factor: 4.162
  7 in total

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