Kirubananthan Gothandam1, Vijayan Siva Ganesan1, Thangaraj Ayyasamy2, Sundaram Ramalingam3,4. 1. a Department of Biotechnology , University of Madras , Chennai , India. 2. b Department of Plant Biology and Plant Biotechnology , Government Arts College for Men (Autonomous), Nandanam, University of Madras , Chennai , India. 3. c Department of Medical Biochemistry , Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras , Chennai , India. 4. d Department of Biochemistry , Saveetha Dental College & Hospital, Saveetha Institute of Medical & Technical Sciences , Chennai , India.
Abstract
Objectives: The present study was to evaluate the effect of theaflavin on the activities of key enzymes of carbohydrate metabolism in high fat diet and streptozotocin - induced diabetic rats. Methods: Diabetes was induced in male albino Wistar rats by feeding them with high fat diet comprising of standard laboratory rat chow 84.3%, lard 5%, egg yolk powder 10%, cholesterol 0.2% and bile salt 0.5% for 2 weeks. After 2 weeks, the animals were kept in an overnight fast and injected with low dose of streptozotocin (40 mg/kg b.w). Results: Theaflavin (100 mg/kg b.w /day) was administered orally to diabetic rats for 30 days. At the end of the experimental period, diabetic control rats showed significant increase in plasma glucose, homeostatic model assessment of insulin resistance (HOMA-IR), glycosylated hemoglobin (HbA1c) with concomitant decrease in plasma insulin, total hemoglobin and body weight. The activities of key enzymes of carbohydrate metabolism, lipid peroxidation markers, antioxidant enzymes, glycogen content and glycogen synthase and glycogen phosphorylase were also altered in diabetic rats. Discussion: Oral administration of theaflavin to diabetic rats significantly ameliorated all the biochemical alterations to near normal levels. The results of the present study suggest that theaflavin exhibits antidiabetic effect through its antioxidant activity.
Objectives: The present study was to evaluate the effect of theaflavin on the activities of key enzymes of carbohydrate metabolism in high fat diet and streptozotocin - induced diabeticrats. Methods:Diabetes was induced in male albino Wistar rats by feeding them with high fat diet comprising of standard laboratory rat chow 84.3%, lard 5%, egg yolk powder 10%, cholesterol 0.2% and bile salt 0.5% for 2 weeks. After 2 weeks, the animals were kept in an overnight fast and injected with low dose of streptozotocin (40 mg/kg b.w). Results:Theaflavin (100 mg/kg b.w /day) was administered orally to diabeticrats for 30 days. At the end of the experimental period, diabetic control rats showed significant increase in plasma glucose, homeostatic model assessment of insulin resistance (HOMA-IR), glycosylated hemoglobin (HbA1c) with concomitant decrease in plasma insulin, total hemoglobin and body weight. The activities of key enzymes of carbohydrate metabolism, lipid peroxidation markers, antioxidant enzymes, glycogen content and glycogen synthase and glycogen phosphorylase were also altered in diabeticrats. Discussion: Oral administration of theaflavin to diabeticrats significantly ameliorated all the biochemical alterations to near normal levels. The results of the present study suggest that theaflavin exhibits antidiabetic effect through its antioxidant activity.
Authors: Mousa O Germoush; Hassan A Elgebaly; Sherif Hassan; Emadeldin M Kamel; May Bin-Jumah; Ayman M Mahmoud Journal: Antioxidants (Basel) Date: 2019-12-26