Vanessa Belebecha1, Rúbia Casagrande2, Mariana R Urbano3, Jefferson Crespigio4, Renata M Martinez2, David L Vale2, Sílvio Henrique Maia de Almeida5. 1. Department of Surgery, Center for Health Sciences, State University of Londrina, Rua das Bromélias, 93, Londrina, PR, 86055-734, Brazil. 2. Department of Pharmaceutical Sciences, State University of Londrina, Londrina, Brazil. 3. Statistics Department, State University of Londrina, Londrina, Brazil. 4. Department of Pathology, State University of Londrina, Londrina, Brazil. 5. Department of Surgery, Center for Health Sciences, State University of Londrina, Rua das Bromélias, 93, Londrina, PR, 86055-734, Brazil. salmeida@sercoomtel.com.br.
Abstract
INTRODUCTION AND HYPOTHESIS: Polypropylene mesh (PPM) is often used for urogynecological repair; however, it can cause complications. An approach to reduce complications is to coat PPM with anti-inflammatory and wound-healing molecules. Platelet-rich plasma (PRP) is inexpensive and improves wound healing. Therefore, we evaluated whether covering PPM with PRP could reduce inflammation, adhesion, and oxidative stress (OS) in rabbits. METHODS: The primary objective was to evaluate OS, and the secondary objectives were to evaluate inflammation and adhesion. PRP-coated PPM was implanted on the right side of the abdominal cavity of 12 female New Zealand rabbits, in the interface between the hypodermis and peritoneum. An uncoverated PPM was implanted in the other side. Twelve rabbits served as the sham group; all animals were euthanized after 30 or 60 days. Inflammatory parameters were myeloperoxidase (MPO) and N-acetylglucosaminidase (NAG) activities. OS was evaluated by measuring the ferric-reducing antioxidant power, the free-radical-reducing ability of 3-ethylbenzothiazoline-6-sulfonic acid [2,2'-azino-bis (ABTS)], reduced glutathione levels, and superoxide anion production. Adhesion was measured using tenacity and Diamond scales (the latter of which grades adhesions according to their extent) Inflammation and OS were analyzed by analysis of variance (ANOVA), followed by Tukey's test. The Mann-Whitney test was used to evaluate adhesions, and analysis of the sham group was conducted using Kruskal-Wallis test. RESULTS: No significant differences were observed in parameters of adhesions. After 60 days, PRP-coverated PPM presented a decrease in MPO and NAG activities. Furthermore, decreased OS and increased antioxidant levels were observed in PRP-coverated PPM samples. CONCLUSIONS: The reduction of OS and inflammatory responses indicates that PRP-covered PPM is a promising therapeutic approach.
INTRODUCTION AND HYPOTHESIS: Polypropylene mesh (PPM) is often used for urogynecological repair; however, it can cause complications. An approach to reduce complications is to coat PPM with anti-inflammatory and wound-healing molecules. Platelet-rich plasma (PRP) is inexpensive and improves wound healing. Therefore, we evaluated whether covering PPM with PRP could reduce inflammation, adhesion, and oxidative stress (OS) in rabbits. METHODS: The primary objective was to evaluate OS, and the secondary objectives were to evaluate inflammation and adhesion. PRP-coated PPM was implanted on the right side of the abdominal cavity of 12 female New Zealand rabbits, in the interface between the hypodermis and peritoneum. An uncoverated PPM was implanted in the other side. Twelve rabbits served as the sham group; all animals were euthanized after 30 or 60 days. Inflammatory parameters were myeloperoxidase (MPO) and N-acetylglucosaminidase (NAG) activities. OS was evaluated by measuring the ferric-reducing antioxidant power, the free-radical-reducing ability of 3-ethylbenzothiazoline-6-sulfonic acid [2,2'-azino-bis (ABTS)], reduced glutathione levels, and superoxide anion production. Adhesion was measured using tenacity and Diamond scales (the latter of which grades adhesions according to their extent) Inflammation and OS were analyzed by analysis of variance (ANOVA), followed by Tukey's test. The Mann-Whitney test was used to evaluate adhesions, and analysis of the sham group was conducted using Kruskal-Wallis test. RESULTS: No significant differences were observed in parameters of adhesions. After 60 days, PRP-coverated PPM presented a decrease in MPO and NAG activities. Furthermore, decreased OS and increased antioxidant levels were observed in PRP-coverated PPM samples. CONCLUSIONS: The reduction of OS and inflammatory responses indicates that PRP-covered PPM is a promising therapeutic approach.
Authors: Sebastian Medel; May Alarab; Hala Kufaishi; Harold Drutz; Oksana Shynlova Journal: Female Pelvic Med Reconstr Surg Date: 2015 Jul-Aug Impact factor: 2.091
Authors: Christopher R Chapple; Francisco Cruz; Xavier Deffieux; Alfredo L Milani; Salvador Arlandis; Walter Artibani; Ricarda M Bauer; Fiona Burkhard; Linda Cardozo; David Castro-Diaz; Jean Nicolas Cornu; Jan Deprest; Alfons Gunnemann; Maria Gyhagen; John Heesakkers; Heinz Koelbl; Sheila MacNeil; Gert Naumann; Jan-Paul W R Roovers; Stefano Salvatore; Karl-Dietrich Sievert; Tufan Tarcan; Frank Van der Aa; Francesco Montorsi; Manfred Wirth; Mohamed Abdel-Fattah Journal: Eur Urol Date: 2017-04-14 Impact factor: 20.096
Authors: Awoniyi O Awonuga; Jimmy Belotte; Suleiman Abuanzeh; Nicole M Fletcher; Michael P Diamond; Ghassan M Saed Journal: Reprod Sci Date: 2014-02-11 Impact factor: 3.060
Authors: Holger Gerullis; Evangelos Georgas; Christoph Eimer; Christian Arndt; Dimitri Barski; Bernhard Lammers; Bernd Klosterhalfen; Mihaly Borós; Thomas Otto Journal: Biomed Res Int Date: 2013-09-17 Impact factor: 3.411