N Bryan1, H Ahswin, N J Smart, Y Bayon, J A Hunt. 1. Clinical Engineering, UKCTE, UKBioTEC, The Institute of Ageing and Chronic Disease, University of Liverpool, Duncan Building, Daulby Street, Liverpool, L69 3GA, UK. n.bryan@liv.ac.uk
Abstract
OBJECTIVES: Evaluation of an in vitro chemiluminescent screen to predict leukocyte ROS in response to surgical materials. DESIGN AND METHODS: 6 surgical meshes; manufacture and knitting variations of polypropylene (PP), polyester terephtalate (PET) and polyglycolic acid (PGA) trialled healthy human blood (n=5). Materials and blood were incubated with pholasin. Pholasin emits photons in the presence of reactive oxygen species; secreted by activated leukocytes. RESULTS: Multifilament-PGA mesh stimulated the greatest ROS response from blood derived human leukocytes. Multifilament-PET light weight and multifilament-PP meshes stimulated similar levels of ROS production which were greater than monofilament-PP light, monofilament-PP and monofilament-PET light meshes. Data demonstrated statistical variations in trans-donor response to the materials. CONCLUSIONS: An in vitro chemiluminescent assay can be used to assess leukocyte respiratory burst response to biomaterials. PGA mesh elicited the greatest ROS response. PP and PET monofilament meshes induce less ROS than multifilament equivalents. In vitro results correlate with previously published clinical responses to these materials.
OBJECTIVES: Evaluation of an in vitro chemiluminescent screen to predict leukocyte ROS in response to surgical materials. DESIGN AND METHODS: 6 surgical meshes; manufacture and knitting variations of polypropylene (PP), polyester terephtalate (PET) and polyglycolic acid (PGA) trialled healthy human blood (n=5). Materials and blood were incubated with pholasin. Pholasin emits photons in the presence of reactive oxygen species; secreted by activated leukocytes. RESULTS: Multifilament-PGA mesh stimulated the greatest ROS response from blood derived human leukocytes. Multifilament-PET light weight and multifilament-PP meshes stimulated similar levels of ROS production which were greater than monofilament-PP light, monofilament-PP and monofilament-PET light meshes. Data demonstrated statistical variations in trans-donor response to the materials. CONCLUSIONS: An in vitro chemiluminescent assay can be used to assess leukocyte respiratory burst response to biomaterials. PGA mesh elicited the greatest ROS response. PP and PET monofilament meshes induce less ROS than multifilament equivalents. In vitro results correlate with previously published clinical responses to these materials.
Authors: Vanessa Belebecha; Rúbia Casagrande; Mariana R Urbano; Jefferson Crespigio; Renata M Martinez; David L Vale; Sílvio Henrique Maia de Almeida Journal: Int Urogynecol J Date: 2019-05-25 Impact factor: 2.894
Authors: Holger Gerullis; Evangelos Georgas; Christoph Eimer; Christian Arndt; Dimitri Barski; Bernhard Lammers; Bernd Klosterhalfen; Mihaly Borós; Thomas Otto Journal: Biomed Res Int Date: 2013-09-17 Impact factor: 3.411