Literature DB >> 31127586

Measuring UV Photoproduct Repair in Isolated Telomeres and Bulk Genomic DNA.

Elise Fouquerel1,2, Ryan P Barnes2, Hong Wang3,4, Patricia L Opresko5.   

Abstract

Telomere repeats at chromosomal ends are essential for genome stability and sustained cellular proliferation but are susceptible to DNA damage. Repair of damage at telomeres is influenced by numerous factors including telomeric binding proteins, sequence and structure. Ultraviolet (UV) light irradiation induces DNA photoproducts at telomeres that can interfere with telomere maintenance. Here we describe a highly sensitive method for quantifying the formation and removal of UV photoproducts in telomeres isolated from UV irradiated cultured human cells. Damage is detected by immunospot blotting of telomeres with highly specific antibodies against UV photoproducts. This method is adaptable for measuring other types of DNA damage at telomeres as well.

Entities:  

Keywords:  Immunospot blot; Radiolabeled DNA probes; Telomere isolation; Telomeres; UV irradiation; UV photoproducts

Mesh：

Substances：

Year:  2019        PMID： 31127586      PMCID： PMC6886745          DOI： 10.1007/978-1-4939-9500-4_20

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

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Review 4.  DNA damage processing at telomeres: The ends justify the means.

Authors:  Elise Fouquerel; Dhvani Parikh; Patricia Opresko
Journal:  DNA Repair (Amst)       Date:  2016-05-16

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Authors:  Mary Armanios; Elizabeth H Blackburn
Journal:  Nat Rev Genet       Date:  2012-09-11       Impact factor: 53.242

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Authors:  Gerdine J Stout; Maria A Blasco
Journal:  Cancer Res       Date:  2013-01-03       Impact factor: 12.701

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Authors:  Wilhelm Palm; Titia de Lange
Journal:  Annu Rev Genet       Date:  2008       Impact factor: 16.830

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Authors:  John J DiGiovanna; Kenneth H Kraemer
Journal:  J Invest Dermatol       Date:  2012-01-05       Impact factor: 8.551

10.  Telomeres are partly shielded from ultraviolet-induced damage and proficient for nucleotide excision repair of photoproducts.

Authors:  Dhvani Parikh; Elise Fouquerel; Connor T Murphy; Hong Wang; Patricia L Opresko
Journal:  Nat Commun       Date:  2015-09-09       Impact factor: 14.919
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  2 in total

Review 1.  Purification of mammalian telomeric DNA for single-molecule analysis.

Authors:  Giulia Mazzucco; Armela Huda; Martina Galli; Elia Zanella; Ylli Doksani
Journal:  Nat Protoc       Date:  2022-04-08       Impact factor: 13.491

Review 2.  The Power of Stress: The Telo-Hormesis Hypothesis.

Authors:  Maria Sol Jacome Burbano; Eric Gilson
Journal:  Cells       Date:  2021-05-11       Impact factor: 6.600
  2 in total

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