| Literature DB >> 31127558 |
Shamroop Kumar Mallela1,2, Devang Maheshkumar Patel1,2, Gloria Michelle Ducasa1,2, Sandra Merscher1,2, Alessia Fornoni3,4, Hassan Al-Ali5,6,7,8.
Abstract
Lipid droplets (LDs) are dynamic organelles that regulate the storage and homeostasis of intracellular triglycerides and other neutral lipids. Studies show that the number, morphology, and subcellular localization of LDs are altered in a growing number of diseases. As such, methodologies for imaging and quantifying LDs have become essential tools for detecting changes in cellular lipid metabolism, which could be an important indicator of disease onset or progression. We previously reported on the accumulation of LDs in podocytes of the kidney glomerulus in nephrological diseases of metabolic and non-metabolic origin. Here, we describe a high-content analysis (HCA) method for automated detection and quantification of LDs in differentiated human podocytes.Entities:
Keywords: Cell-based assay; High content screening; Human podocytes; Lipid droplets; Phenotypic drug discovery
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Year: 2019 PMID： 31127558 PMCID： PMC7941729 DOI： 10.1007/978-1-4939-9488-5_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745