Polyketides from the Mangrove-Derived Endophytic Fungus Cladosporium cladosporioides.

Five new polyketides, namely, 5R-hydroxyrecifeiolide (1), 5S-hydroxyrecifeiolide (2), ent-cladospolide F (3), cladospolide G (4), and cladospolide H (5), along with two known compounds (6 and 7), were isolated from the endophytic fungal strain Cladosporium cladosporioides MA-299 that was obtained from the leaves of the mangrove plant Bruguiera gymnorrhiza. The structures of these compounds were established by extensive analysis of 1D/2D NMR data, mass spectrometric data, ECDs and optical rotations, and modified Mosher's method. The structures of 3 and 6 were confirmed by single-crystal X-ray diffraction analysis and this is the first time for reporting the crystal structures of these two compounds. All of the isolated compounds were examined for antimicrobial activities against human and aquatic bacteria and plant pathogenic fungi as well as enzymatic inhibitory activities against acetylcholinesterase. Compounds 1-4, 6, and 7 exhibited antimicrobial activity against some of the tested strains with MIC values ranging from 1.0 to 64 μg/mL, while 3 exhibited enzymatic inhibitory activity against acetylcholinesterase with the IC50 value of 40.26 μM.

1. Introduction

The Cladosporium fungi, one of the largest genera of dematiaceous hyphomycetes, have attracted considerable attention of natural products researchers in recent years [1,2]. Versatile bioactive metabolites, such as cladosporin [3], macrolide [4], sulfur-containing diketopiperazines [5], indole alkaloids [6], hybrid polyketides [7], and diterpenes with 5-8-5 ring system [8], have been isolated from the Cladosporium strains. As part of our research on discovering structurally novel and biologically active natural products, a series of interesting metabolites have been obtained from marine-derived fungal strains [9,10], including those from Cladosporium species [11]. Our current chemical investigation on C. cladosporioides MA-299, an endophytic fungus obtained from the fresh inner leaves of the marine mangrove plant Bruguiera gymnorrhiza, led to the discovery of five new polyketides, namely, 5R-hydroxyrecifeiolide (1), 5S-hydroxyrecifeiolide (2), ent-cladospolide F (3) [12], cladospolide G (4), and cladospolide H (5) (Figure 1), as well as two known analogues, including iso-cladospolide B (6) [13,14], and pandangolide 1 (7) [13,15] (Figure 1). Herein, we report the isolation, structure assignment, and biological evaluation of the isolated compounds.

Figure 1

Structures of the isolated compounds 1–7.

2. Results and Discussion

2.1. Structure Elucidation of the New Compounds

5R-Hydroxyrecifeiolide (1) was isolated as a colorless oil and the molecular formula C12H20O3 was deduced from the (+)-HRESIMS data, indicating three degrees of unsaturation. The 1H and 13C NMR spectra of 1 showed the signals for one ester/lactone carbonyl, two olefinic and two oxygenated sp3 methines, six sp3 methylenes, and one methyl group (Table 1). In addition, the 1H NMR data of 1 were quite similar to those of recifeiolide (11-hydroxy-trans-8-dodecenoic acid lactone) [16,17], except that one methylene (δH 1.5–2.3 ppm) in recifeiolide was replaced by an oxygenated methine (δH 3.51 ppm) in 1. The key COSY correlations elucidated the connectivity from H-2 through H-12 (Figure 2). Key HMBC correlations from H-2 to C-1 and C-4, from H-3 to C-1 and C-5, and from H-11 to C-1, connected C-1 and C-2 and determined the 12-membered macrolide skeleton of 1 (Figure 2). The relative configuration at C-5 and C-11 for 1 was established by the NOESY experiment (Figure S8). The NOESY correlations (Figure 3) from H-2β to H-5 and H-11 revealed a β orientation of these protons [18]. The coupling constants between H-8 and H-9 (JH-8/H-9 = 15.3 Hz) suggested the E-configuration of the C-8/C-9 double bond. The absolute configuration of C-5 of 1 was assigned by application of the modified Mosher’s method [19]. The ∆δ values obtained for the (S)- and (R)-MTPA esters (1a and 1b, respectively) of 1 (Figure 4) suggested that the absolute configuration of C-5 is R. Furthermore, the electronic circular dichroism (ECD) spectrum of 1 was recorded and then computed with the time-dependent density function theory (TD-DFT) method at the gas-phase B3LYP/6-31G (d) level [20,21]. The calculated ECD spectra were produced by SpecDis software [22]. The experimental ECD spectrum for 1 matched well with the calculated spectrum for 11R (Figure 5). Therefore, the 5R, 11R configuration of 1 was established, and the trivial name 5R-hydroxyrecifeiolide was assigned.

Table 1

1H and 13C NMR data of 1, 2, and 7 (δ in ppm).

Pos.	1			2		7
	a δH (J in Hz)	b δH (J in Hz)	d δ C	b δH (J in Hz)	e δ C	c δH (J in Hz)	f δ C
1			172.3, C		172.2, C		174.7, C
2	α 2.40, ddd (13.0, 5.6, 3.9)β 1.93, ddd (13.1, 11.0, 4.4)	α 2.40, ddd (13.0, 5.6, 3.9)β 1.93, ddd (13.1, 11.0, 4.4)	35.2, CH2	α 2.25, m (overlap)β 2.16, m	31.8, CH2	α 3.23, dd (18.5, 8.5)β 2.90, dd (18.5, 1.7)	43.0, CH2
3	1.56, m (overlap)	1.56, m (overlap)	18.2, CH2	α 1.67, m (overlap)β 1.54, m	19.9, CH2	4.72, dd (8.5, 1.7)	65.7, CH
4	1.43, m (overlap)	1.43, m (overlap)	32.9, CH2	α 1.41, mβ 0.97, m	30.0, CH2		209.3, C
5	3.51, m	3.51, m	70.2, CH	3.48, m	65.4, CH	4.31, d (5.4)	76.3, CH
6	α 1.60, m (overlap)β 1.35, m (overlap)	α 1.60, m (overlap)β 1.35, m (overlap)	32.6, CH2	α 1.63, m (overlap)β 1.30, m	33.7, CH2	α 1.97, mβ 1.76, m	30.7, CH2
7	α 2.17, mβ 1.72, m	α 2.17, mβ 1.72, m	28.6, CH2	α 2.05, mβ 1.91, m	27.7, CH2	α 1.45, m (overlap)β 1.18, m (overlap)	20.8, CH2
8	5.34, ddd (15.3, 10.7, 2.8)	5.34, ddd (15.3, 10.7, 2.8)	135.2, CH	5.26, m	132.7, CH	α 1.60, m (overlap)β 1.31, m (overlap)	26.9, CH2
9	5.20, ddd (15.3, 10.1, 4.1)	5.20, ddd (15.3, 10.1, 4.1)	125.6, CH	5.11, m	126.7, CH	α 1.51, m (overlap)β 1.10, m (overlap)	22.7, CH2
10	α 2.25, mβ 2.06, m	α 2.25, mβ 2.06, m	40.3, CH2	α 2.29, m (overlap)β 1.99, m (overlap)	40.3, CH2	α 1.69, m (overlap)β 1.39, m (overlap)	33.5, CH2
11	4.84, m	4.84, m	68.3, CH	5.02, m	67.8, CH	4.88, m	75.2, CH
12	1.17, d (6.3)	1.17, d (6.3)	20.4, CH3	1.15, d (6.3)	20.2, CH3	1.25, d (6.2)	19.3, CH3
3-OH						3.19, s
5-OH	4.39, brs	4.39, brs		4.28, brs		3.03, s

Measured at 600 MHz in DMSO-d6. Measured at 500 MHz in DMSO-d6. Measured at 500 MHz in CDCl3. Measured at 150 MHz in DMSO-d6. Measured at 125 MHz in DMSO-d6. Measured at 125 MHz in CDCl3.

Figure 2

Key COSY (bold lines) and HMBC (red arrows) correlations for 1–6.

Figure 3

Key NOESY correlations for 1 and 5.

Figure 4

∆δ values (∆δ (in ppm) = δ − δ) obtained for the (S)-and (R)-MTPA esters (1a and 1b, respectively) of 1.

Figure 5

Comparison of experimental and calculated ECD spectra of 1 and 2.

The molecular formula of 2 was determined as C12H20O3, which was the same as that of 1, according to its (+)-HRESIMS data. The 1H and 13C NMR spectra (Table 1) of 2 were similar to those of 1, except for the different 13C chemical shifts at C-5 (δC 70.2 in 1, and δC 65.4 in 2) and its adjacent positions (C-2–C-4 and C-6–C-8), which indicated that compound 2 was the 5-epimer of 1. The chemical shifts at C-2 and C-8 (γ-position of C-5) exhibited obvious difference in 2 and 1 probably due to the space effect. As expected, the experimental ECD spectrum of 2 matched well with the calculated spectrum of 11R (Figure 5). The trivial name 5S-hydroxyrecifeiolide was assigned to 2.

Compound 3 was initially obtained as pale yellow powder and possessed a molecular formula C12H22O4 by (+)-HRESIMS, implying two degrees of unsaturation. The 1H and 13C NMR data (Table 2) exhibited signals attributed to one ester carbonyl, three oxygenated sp3 methines, seven sp3 methylenes, and one methyl group. These data were very similar to those of cladospolide F [12], suggesting that they had the same planar structure, which was also confirmed by the COSY and HMBC correlations (Figure 2). However, the signs of the optical rotations of 3 (−29.41, MeOH) and cladospolide F (+15.7, MeOH) were opposite, indicating that the absolute configurations of their stereogenic carbons were different. The relative configuration at C-3, C-4, and C-11 could not be concluded by NOESY experiment. Nevertheless, suitable crystals were obtained for X-ray diffraction analysis using Cu Kα radiation which confirmed the absolute configuration of C-3, C-4 and C-11 as 3R, 4S, and 11R (Figure 6). The ent-cladospolide F was therefore assigned as a trivial name for 3.

Table 2

1H and 13C NMR data of 3–6 (δ in ppm).

Pos.	3				4		5		6
	a δH (J in Hz)	b δH (J in Hz)	d δ C	e δ C	c δH (J in Hz)	f δ C	a δH (J in Hz)	d δ C	a δH (J in Hz)	d δ C
1			175.5, C	175.8, C		175.5, C		169.7, C		173.2, C
2	α 2.85, dd (17.7, 6.4)β 2.25, dd (17.7, 3.2)	α 2.78, dd (18.0, 6.7)β 2.47, dd (18.0, 3.7)	37.0, CH2	37.8, CH2	α 2.85, dd (17.7, 6.4)β 2.25, dd (17.7, 3.2)	37.0, CH2	6.37, d (5.4)	118.4, CH	6.21, d (5.6)	121.1, CH
3	4.09, m	4.22, m	70.1, CH	71.7, CH	4.09, m	70.1, CH	7.80, d (5.4)	145.1, CH	7.71, d (5.6)	156.6, CH
4	4.18, m	4.31, ddd (8.3, 5.3, 3.0)	87.5, CH	88.2, CH	4.18, ddd (8.1, 5.5, 2.5)	87.5, CH		149.4, C	5.04, d (1.5)	86.2, CH
5	α 1.56, mβ 1.48, m	1.57, m	32.1, CH2	33.2, CH2	α 1.57, mβ 1.48, m (overlap)	32.0, CH2	5.53, t (7.9)	117.1, CH	3.66, m	69.4, CH
6	1.26, m (overlap)	1.34, m (overlap)	25.2, CH2	25.7, CH2	1.25, m (overlap)	24.7, CH2	2.30, m	25.8, CH2	1.44, m (overlap)	40.0, CH2
7	1.33, m (overlap)	1.41, m (overlap)	29.0, CH2	29.5, CH2	1.33, m (overlap)	28.6, CH2	1.44, m	28.3, CH2	1.34, m (overlap)	32.8, CH2
8	1.31, m (overlap)	1.28, m (overlap)	28.8, CH2	29.3, CH2	1.31, m (overlap)	28.5, CH2	1.23, m (overlap)	28.7, CH2	1.29, m (overlap)	29.1, CH2
9	1.24, m (overlap)	1.26, m (overlap)	24.8, CH2	25.3, CH2	1.24, m (overlap)	24.6, CH2	1.27, m (overlap)	25.0, CH2	1.25, m (overlap)	25.3, CH2
10	1.36, m (overlap)	1.45, m (overlap)	39.0, CH2	39.3, CH2	1.45, m (overlap)	35.2, CH2	1.32, m (overlap)	38.9, CH2	1.39, m (overlap)	39.0, CH2
11	3.55, m	3.75, m	65.7, CH	68.3, CH	4.78, m	70.1, CH	3.55, m (overlap)	65.6, CH	3.55, m	65.7, CH
12	1.02, d (6.1)	1.15, d (6.2)	23.6, CH3	23.7, CH3	1.15, d (6.3)	19.7, CH3	1.02, d (6.1)	23.6, CH3	1.02, d (6.2)	23.6, CH3
13						169.9, C
14					1.97, s	21.0, CH3
3-OH	5.49, s				5.50, d (4.0)
5-OH									4.95, d (6.3)
11-OH	4.27, d (4.2)						3.41, brs		4.29, d (4.7)

Measured at 500 MHz in DMSO-d6. Measured at 500 MHz in CDCl3. Measured at 600 MHz in DMSO-d6. Measured at 125 MHz in DMSO-d6. Measured at 125 MHz in CDCl3. Measured at 150 MHz in DMSO-d6.

Figure 6

Ortep diagrams of ent-cladospolide F (3) and iso-cladospolide B (6).

Compound 4 was obtained as a pale yellow oil and its molecular formula was determined as C14H24O5 on the basis of (+)-HRESIMS, requiring three degrees of unsaturation. The 1H and 13C NMR data for 4 (Table 2) were quite similar to those of 3, except for the presence of additional ester carbonyl (C-13) and methyl (C-14) groups, which indicated the replacement of 11-OH group in 3 by an OAc group in 4, and thus caused the down-field shift of 11-H from δH 3.55 in 3 to δH 4.78 in 4. Detailed interpretation of the COSY and HMBC spectra revealed that 4 was an analogue of 3, with the hydroxyl group at C-11 in 3 being replaced by an acetoxyl group in 4. The HMBC correlation from H-11 to C-13 established the presence of an acetoxyl group at C-11, and the planar structure of 4 was hence confirmed as shown (Figure 2). In a biogenetic perspective, it was tentatively assigned the same relative configuration as that of 3. The similar optical rotations of 4 (−24.56, MeOH) and 3 (−29.41, MeOH) also supported that the absolute configurations of the stereogenic carbons in 4 were the same as those in 3. Therefore, the absolute configurations of the stereogenic carbons in 4 were tentatively assigned as 3R, 4S, and 11R, and the trivial name cladospolide G was assigned. Acetylation of compounds 3 and 4 using acetyl chloride yielded the same diacetylated derivative, which further correlated the structure relationship of compounds 3 and 4.

Compound 6 was isolated as colorless crystals and gave ion peaks at m/z 229.1432 [M + H]+ and 246.1699 [M + NH4]+ in the (+)-HRESIMS, corresponding to a molecular formula C12H20O4, indicating three degrees of unsaturation. All the 1H and 13C NMR data of 6 were quite similar to those of the previously reported polyketide metabolite iso-cladospolide B [13]. The COSY and HMBC correlations (Figure 2) confirmed that the planar structure of 6 was the same as that of iso-cladospolide B. The high similarity of specific rotations of 6 ( = −90.91 (c 0.11, MeOH) ) and iso-cladospolide B (= −90 (c 0.23, MeOH)) [13] suggested that they may have the same relative and absolute stereochemistry. However, neither the relative nor the absolute configuration was determined [13]. In 2001, Franck et al. carried out the first synthesis of iso-cladospolide B and proposed that it has the 4S, 5S, and 11R configuration [14]. Later, in 2005, the absolute configuration of iso-cladospolide B, isolated from Cladosporium sp. isolated from the Red Sea sponge Niphates rowi, was assigned to be 4S, 5S, and 11S ( = −61 (c 16.6, MeOH)) [15]. It was later stated that both diastereomers appear to be natural products and (4S, 5S, 11S)-isomer referred to as 11-epi-iso-cladospolide B [23,24]. The relative configuration of 6 could not be assigned by NOESY experiments but the coupling constant for C-4 (J = 1.5 Hz) confirmed the threo relative configuration [14]. Upon slow evaporation of the solvent (MeOH-H2O), compound 6 was crystallized and the X-ray analysis was carried out, which was first reported for iso-cladospolide B (Figure 4). The Cu Kα Flack parameter 0.5 (7) allowed preliminary confirmation of the relative configurations of 6 as 4S*, 5S*, 11R*.

Compound 5 was obtained as a pale yellow oil and possessed a molecular formula of C12H18O3 by (+)-HRESIMS, implying four degrees of unsaturation. The 1D NMR data (Table 2) and HSQC spectrum (Figure S38) suggested signals attributed to one ester and one olefinic quaternary carbons, one oxygenated and three olefinic methines, five sp3 methylenes, and one methyl group. These NMR data were similar to those of iso-cladospolide B (6) [13]. However, resonances for two oxygenated methines (C-4 and C-5) in 6 were not detected in the NMR spectra of 5. Instead, two additional olefinic signals including one quaternary sp2 (C-4, δC 149.4) and one methine sp2 (C-5, δC 117.1/δH 5.53) carbons were observed in the NMR spectra of 5 (Table 2). These data indicated that 5 was a reduced analogue of 6, and this deduction was supported by the molecular formula. The COSY and HMBC spectra established the structure of 5 as shown in Figure 1. In the NOESY experiment, the correlation between H-3 and H-5 indicated the Z-conformation of the double bond between C-4 and C-5 (Figure 3). The absolute stereochemistry of 5 could not be determined by Mosher’s method because of the limited amount of material available. From a biogenetic point of view, 5 was putatively produced by reduction of 6. Therefore, it was tentatively assigned the absolute configuration of C-11 of 5 as 11R. From these data, the name cladospolide H was assigned for 5.

Compound 7 was acquired as white powder and showed ion peaks at m/z 267.1197 [M + Na]+ in the positive HRESIMS, corresponding to a molecular formula of C12H20O5. A literature search indicated that all the 1H and 13C NMR data of 7 were almost the same as those of previously reported compound pandangolide 1 [13,15]. The almost exactly the same specific rotations of 7 ( = −30.16 (c 1.22, MeOH)) and pandangolide 1 (= −30 (c 2.3, MeOH)) [15] revealed that they may have the same relative and absolute configurations.

2.2. Biological Activities of the Isolated Compounds

Compounds 1–7 were tested for antimicrobial activities against two human pathogens (Escherichia coli, Staphylococcus aureus), ten aquatic bacteria (Aeromonas hydrophilia, Edwardsiella ictarda, E. tarda, Micrococcus luteus, Pseudomonas aeruginosa, Vibrio alginolyticus, V. anguillarum, V. harveyi, V. parahaemolyticus, and V. vulnificus), and 15 plant pathogenic fungi (Alternaria solani, Bipolaris sorokiniana, Ceratobasidium cornigerum, Colletotrichum glecosporioides, Coniothyrium diplodiella, Fusarium graminearum, F. oxysporum f. sp. cucumerinum, F. oxysporum f. sp. momodicae, F. oxysporum f. sp. radicis lycopersici, F. solani, Glomerella cingulate, Helminthosporium maydis, Penicillium digitatum, Physalospora piricola Nose, and Valsa mali). As shown in Table 3, 3 exhibited moderate inhibitory activities against human pathogenic bacteria S. aureus with MIC value of 8.0 μg/mL. Compound 4 showed potent inhibitory activities against plant-pathogenic fungi (G. cingulate and F. oxysporum f. sp. cucumerinum), each with an MIC value of 1.0 μg/mL, while 7 showed activity against aquatic bacterium (E. ictarda) and plant-pathogenic fungus (G. cingulate), with MIC values of 4.0 and 1.0 μg/mL, respectively.

Table 3

Antimicrobial Activities of 1–7 (MIC, μg/mL) a.

Strains	Compounds
	1	2	3	4	6	7	Positive control
E. colib	–	–	–	32	32	–	2.0
S. aureusb	–	–	8.0	–	–	32	1.0
E. tardab	–	–	–	–	32	–	0.5
E. ictardab	32	–	16	–	16	4.0	0.5
G. cingulatec	–	16	–	1.0	64	1.0	0.5
B. sorokinianac	–	–	–	32	–	–	0.5
P. aeruginosac	32	–	64	–	–	32	2.0
F.oxysporum f. sp. Cucumerinumc	–	–	–	1.0	–	–	0.5

(–) = MIC > 64 μg/mL, Chloramphenicol as positive control, Amphotericin B as positive control.

Compounds 1–7 were also evaluated for acetylcholinesterase inhibitory activity. Compound 3 exhibited potent activity against acetylcholinesterase with the IC50 value of 40.26 μM. The other compounds have a weak activity (IC50 > 50 μM).

3. Experimental Section

3.1. General Experimental Procedures

Melting points were determined by an SGW X-4 micro-melting-point apparatus (Shanghai Shenguang Instrument Co. Ltd, Shanghai, China). Optical rotations were measured on an Optical Activity AA-55 polarimeter (Optical Activity Ltd., Cambridgeshire, UK). UV spectra were measured on a PuXi TU-1810 UV-visible spectrophotometer (Shanghai Lengguang Technology Co. Ltd., Shanghai, China). ECD spectra were acquired on a Chirascan spectropolarimeter (Applied Photophysics Ltd., Leatherhead, UK). The 1H, 13C, and 2D NMR spectra were acquired using a Bruker Avance 500 or 600 M spectrometer (Bruker Biospin Group, Karlsruhe, Germany). Chemical shifts (δ) were expressed in ppm with reference to the solvent peaks (13C, CDCl3: 77.16 ppm, DMSO-d6: 39.52 ppm; 1H, CDCl3: 7.26 ppm, DMSO-d6: 2.50 ppm). Mass spectra were obtained from an API QSTAR Pulsar 1 mass spectrometer (Applied Biosystems, Foster, Waltham, MA, USA). Analytical HPLC analyses were performed using a Dionex HPLC system (Dionex, Sunnyvale, CA, USA) equipped with P680 pump, ASI-100 automated sample injector, and UVD340U multiple wavelength detector controlled by Chromeleon software (version 6.80). Column chromatography (CC) was performed with silica gel (200–300 mesh, Qingdao Haiyang Chemical Factory, Qingdao, China), Lobar LiChroprep RP-18 (40–60 μm, Merck, Darmstadt, Germany), and Sephadex LH-20 (18–110 μm, Merck).

3.2. Fungal Material

The fungal strain Cladosporium cladosporioides MA-299 was isolated from the leaves of the mangrove plant Bruguiera gymnorrhiza, collected in Hainan Island, China, in March 2015. The strain was identified as Cladosporium cladosporioides by analysis of its ITS region of the rDNA, which is the same (100%) as that of C. cladosporioides DCF-1 (accession no. MG208055). The sequence data were deposited in GenBank with the accession number MH822624. The strain is preserved at Key Laboratory of Experimental Marine Biology, Institute of Oceanology of the Chinese Academy of Sciences (IOCAS).

3.3. Fermentation

For chemical investigations, the strain of C. cladosporioides MA-299 was cultured on PDA (Potato Dextrose Agar) medium at 28 °C for six days and then inoculated into 100 × 1 L flasks, each containing 70 g of rice, 0.1 g corn syrup, 0.3 g peptone, 0.1 g methionine and 100 mL seawater that was obtained from the Huiquan Gulf of the Yellow Sea near the campus of IOCAS, statically cultured for 48 days at room temperature.

3.4. Extraction and Isolation

After 48 days, the fermented rice substrate was mechanically fragmented and then extracted three times with 300 mL EtOAc every flask. All of the EtOAc extracts were filtered and evaporated under reduced pressure to yield a crude extract (52.3 g).

The crude extract was subjected to a silica gel vacuum liquid chromatography (VLC), eluting with different solvents of increasing polarity from petroleum ether (PE) to MeOH to yield ten fractions (Frs. 1–10) based on TLC and HPLC analysis. Fr. 5 (2.1 g) was further purified by reversed-phase column chromatography (CC) over Lobar LiChroprep RP-18 with a MeOH-H2O gradient (from 10: 90 to 100: 0) to afford four subfractions (Frs. 5.1–5.4). Fr. 5.2 was further purified by CC on Sephadex LH–20 (MeOH) and then by preparative TLC (plate: 20 × 20 cm, developing solvents: CH2Cl2/MeOH, 30: 1) to obtain 5 (2.6 mg). Fr. 5.3 was subjected to CC on silica gel eluted with CH2Cl2-MeOH (100:1 to 5:1) to obtain 1 (4.1 mg) and 2 (3.0 mg). Fr. 6 (1.7 g) was further fractionated by CC over Lobar LiChroprep RP-18 with a MeOH/H2O gradient (from 10:90 to 100:0) to yield six subfractions (Frs. 6.1–6.6). Fr. 6.1 (112.4 mg) was further purified by prep. TLC (plate: 20 × 20 cm, developing solvents: petroleum ether/acetone, 2:1) and then on Sephadex LH–20 (MeOH) to obtain 7 (3.2 mg). Fr. 6.5 was subjected to CC on silica gel eluted with CH2Cl2-MeOH (150:1 to 70:1) to obtain 4 (5.7 mg). Further purification of Fr. 7 (3.6 g) by CC over Lobar LiChroprep RP-18 with a MeOH/H2O gradient (from 10:90 to 100:0) yielded seven subfractions (Frs. 7.1–7.7). Fr. 7.1 (736.2 mg) was purified by CC on silica gel eluting with a petroleum ether-acetone gradient (from 10:1 to 2:1), and further fractionated by Sephadex LH-20 (MeOH) to afford 3 (91.6 mg). Fr. 7.2 (960.7 mg) was further separated by CC on silica gel eluting with a petroleum ether-acetone gradient (from 10:1 to 1:1) purification, to afford 6 (23.4 mg).

5R-Hydroxyrecifeiolide (1): Colourless oil; +33.33 (c 0.09, MeOH); UV (MeOH) λmax (log ε) 205 (3.06), 220 (3.01); ECD (7.55 mM, MeOH) λmax (Δε) 233 (−0.01) nm; 1H and 13C NMR data, see Table 1; ESIMS m/z 235 [M + Na]+; (+)-HRESIMS at m/z 235.1298 [M + Na]+ (calcd for C12H20O3Na, 235.1305).

5S-hydroxyrecifeiolide (2): Colourless oil; +23.07 (c 0.13, MeOH); UV (MeOH) λmax (log ε) 205 (3.01), 220 (3.06); ECD (8.49 mM, MeOH) λmax (Δε) 230 (−0.03) nm; 1H and 13C NMR data, see Table 1; ESIMS m/z 235 [M + Na]+; (+)-HRESIMS at m/z 235.1299 [M + Na]+ (calcd for C12H20O3Na, 235.1305).

ent-Cladospolide F (3): Colorless crystal (MeOH); mp 59–62 °C; −29.41 (c 0.17, MeOH); UV (MeOH) λmax (log ε) 206 (3.42); ECD (7.82 mM, MeOH) λmax (Δε) 210 (+0.28) nm, 267 (+0.03) nm; 1H and 13C NMR data, see Table 2; ESIMS m/z 231 [M + H]+, m/z 253 [M + Na]+; (+)-HRESIMS at m/z 231.1589 [M + H]+ (calcd for C12H23O4, m/z 231.1591), at m/z 253.1407 [M + Na]+ (calcd for C12H22O4Na, m/z 253.1410).

Cladospolide G (4): Yellow oil; –24.56 (c 0.57, MeOH); UV (MeOH) λmax (log ε) 206 (3.49), 220 (3.27), 275 (2.62); ECD (4.04 mM, MeOH) λmax (Δε) 207 (+0.80) nm, 323 (−0.10) nm; 1H and 13C NMR data, see Table 2; ESIMS m/z 273 [M + H]+, m/z 295 [M + Na]+; (+)-HRESIMS at m/z 273.1700 [M + H]+ (calcd for C14H25O5, m/z 273.1697), at m/z 290.1970 [M + NH4]+ (calcd for C14H28O5N, m/z 290.1962), at m/z 295.1515 [M + Na]+ (calcd for C14H24O5Na, m/z 295.1516).

Cladospolide H (5): pale yellow oil; 1H and 13C NMR data, see Table 2; ESIMS m/z 233 [M + Na]+; (+)-HRESIMS at m/z 233.1151 [M + Na]+ (calcd for C12H18O3Na, m/z 233.1148). (The optical rotation and ECD of 5 could not be detected due to the limited quantity).

Iso-cladospolide B (6): colorless crystal (MeOH); mp 105–112 °C; −90.91 (c 0.11, MeOH); UV (MeOH) λmax (log ε); ECD (9.21 mM, MeOH) λmax (Δε) 213 (−5.69) nm; 1H and 13C NMR data, see Table 2; (+)-HRESIMS at m/z 229.1432 [M + H]+ (calcd for C12H21O4, 229.1434), at m/z 246.1699 [M + NH4]+ (calcd for C12H24O4N, 246.1700).

3.5. X-Ray Crystallographic Analysis of Compounds and

All crystallographic data were collected on an Agilent Xcalibur Eos Gemini CCD plate diffractometer, using graphite monochromatized Cu/Kα radiation (λ= 1.54178 Å) [25]. The data were corrected for absorption by using the program SADABS [26]. The structures were solved by direct methods with the SHELXTL software package [27]. All nonhydrogen atoms were refined anisotropically. The H atoms were located by geometrical calculations, and their positions and thermal parameters were fixed during the structure refinement. The structure was refined by full-matrix least-squares techniques [28].

Crystal data for compound3: C12H22O4, F.W. = 230.30, Orthorhombic space group P2(1)2(1)2(1), unit cell dimensions a = 5.4655(4) Å, b = 5.5812(6) Å, c = 41.275(3) Å, V = 1259.06(19) Å3, α =β =γ = 90°, Z = 4, dcalcd = 1.215 mg/m3, crystal dimensions 0.40 × 0.28 × 0.10 mm3, μ = 0.734 mm–1, F(000) = 504. The 2385 measurements yielded 1827 independent reflections after equivalent data were averaged, and Lorentz and polarization corrections were applied. The final refinement gave R1 = 0.0487 and wR2 = 0.0970 (I > 2σ(I)). The Flack parameter was 0.0 (5) in the final refinement for all 1827 reflections with 147 Friedel pairs.

Crystal data for compound6: C12H20O4, F.W. = 228.13, Orthorhombic space group P2(1)2(1)2(1), unit cell dimensions a = 5.5217(5) Å, b = 7.6778(7) Å, c = 28.947(2) Å, V = 1227.17(19) Å3, α =β =γ = 90°, Z = 6, dcalcd = 1.236 mg/m3, crystal dimensions 0.35 × 0.24 × 0.16 mm3, μ = 0.752 mm–1, F(000) = 496. The 5282 measurements yielded 2081 independent reflections after equivalent data were averaged, and Lorentz and polarization corrections were applied. The final refinement gave R1 = 0.0727 and wR2 = 0.1620 (I > 2σ(I)). The Flack parameter was 0.5 (7) in the final refinement for all 2081 reflections with 150 Friedel pairs.

3.6. Acetylation of Compounds and

To 5 μmol samples of compound 3 or 4 in glass-stoppered flask were added 400 μL dichloromethane, then excess amount of triethylamine was added. Drip 20 μmol of acetylchloride slowly into the flask in ice bath and keeping the reaction for 12 h. Then stop the reaction by adding 20 μL of water into the flask. The progress of the reaction was monitored by TLC analysis. The resulting reaction mixture was extracted with dichloromethane (2 × 400 μL), dried with Na2SO4, and concentrated in vacuo to obtain the product.

3.7. Antimicrobial Assay

Antimicrobial evaluation against two human pathogens (Escherichia coli EMBLC-1, Staphylococcus aureus EMBLC-2) and ten aquatic pathogens (Aeromonas hydrophilia QDIO-1, Edwardsiella ictarda QDIO-9, E. tarda QDIO-2, Micrococcus luteus QDIO-3, Pseudomonas aeruginosa QDIO-4, Vibrio alginolyticus QDIO-5, V. anguillarum QDIO-6, V. harveyi QDIO-7, V. parahaemolyticus QDIO-8, and V. vulnificus QDIO-10), as well as 15 plant-pathogenic fungi (Alternaria solani QDAU-1, Bipolaris sorokiniana QDAU-5, Ceratobasidium cornigerum QDAU-6, Colletotrichum glecosporioides QDAU-2, Coniothyrium diplodiella QDAU-7, Fusarium graminearum QDAU-4, F. oxysporum f. sp. cucumerinum QDAU-8, F. oxysporum f. sp. momodicae QDAU-9, F. oxysporum f. sp. radicis lycopersici QDAU-10, F. solani QDAU-11, Glomerella cingulate QDAU-12, Helminthosporium maydis QDAU-15, Penicillium digitatum QDAU-14, Physalospora piricola Nose QDAU-15, and Valsa mali QDAU-16), was carried out by the 96-well microtiter plates assay [29]. The pathogens were obtained from the Institute of Oceanology, Chinese Academy of Sciences. Chloramphenicol and amphotericin were used as positive controls for bacteria and fungi, respectively. All of the tested compounds and controls were dissolved in DMSO.

3.8. Enzyme inhibitory Assay

A modified Ellman’s method [30] was used to evaluate AChE inhibitory activities of compounds 1–7 in 96-well microplates. Tacrine was used as the standard inhibitor, and control test was performed without the presence of AChE inhibitors. All the inhibitors, solubilized in MeOH, were diluted stepwise from initial concentration of 32 μM. Every experiment was performed in triplicate. 5 μL inhibitor was added to each well and dried, then 50 μL phosphate buffer (PBS, 10 × 0.01 M, pH 7.2–7.4) was dispensed followed by 10 μL AChE (2 U/mL) and 20 μL 5,5-dithiobis 2-nitrobenzoic acid (DTNB, 5 mM). After 10 min culturing at 37 °C, 20 μL acetylthiocholine iodide (ATCh, 10 mM) was added and then OD was read at 405 nm over another period of 10 min culturing at 37 °C. The enzymatic inhibitory activity was calculated according to the following equation: Inhibition % = ((C − Cbackgroud) − (A − Abackgroud))/(C − Cbackgroud) ×100%, where C is the OD value of the control and A is the OD value in the presence of the inhibitor. As for the background, ATCh was replaced by PBS in A and C and bovine albumin (BSA, 1mg/mL) took the place of AChE in C.

4. Conclusions

In summary, five new compounds (1–5) and two previously reported metabolites (6 and 7) were isolated from the mangrove-derived endophytic fungus C. cladosporioides MA-299. The structures of 3 and 6 were confirmed by single-crystal X-ray diffraction analysis and this is the first time for reporting the crystal structures of the two compounds. Compound 4 showed potent inhibitory activity against plant-pathogenic fungi (G. cingulate and F.oxysporum f. sp. cucumerinum), each with MIC value of 1.0 μg/mL, while 7 showed potent inhibitory activity against aquatic bacterium (E. ictarda) and plant-pathogenic fungus (G. cingulate), with MIC values of 4.0 and 1.0 μg/mL respectively. Compound 3 exhibited moderate inhibitory activity against human pathogenic bacterium S. aureus with MIC value of 8.0 μg/mL and acetylcholinesterase inhibitory activity with IC50 value of 40.26 μM.