Qin Zeng1, Xiyuan Liu2, Chen Chen1, Xiaobo Sun3, Huijuan Li1, Yifan Zhou1, ChunPing Cui1, Yuge Wang1, Yu Yang1, Aimin Wu1, Yaqing Shu1, Xueqiang Hu1, Zhengqi Lu1, Song Guo Zheng4, Wei Qiu1, Yongjun Lu5. 1. Multiple Sclerosis Centre, Department of Neurology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou Guangdong, 510630, China. 2. School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China; Biomedical Center, Sun Yat-sen University, Guangzhou, 510275, China. 3. Department of Clinical Immunology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou Guangdong, 510630, China. 4. Department of Internal Medicine, Ohio State University Wexner Medical Center, Columbus, OH, 43210, USA. 5. School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China; Biomedical Center, Sun Yat-sen University, Guangzhou, 510275, China. Electronic address: luyj@mail.sysu.edu.cn.
Abstract
BACKGROUND: Recent studies, mostly conducted in Western countries, showed that gut microbes are involved in the pathogenesis of multiple sclerosis (MS). OBJECTIVE: The aim of this study was to investigate whether gut dysbiosis is relevant to the initiation and progression of MS in a Chinese population. METHODS: Next-generation sequencing (NGS) and gas chromatography (GC) were integrated and used to compare the fecal bacterial communities and the short-chain fatty acid (SCFA) levels among relapsing-remitting MS (RRMS) patients (n = 34), neuromyelitis optica spectrum disorder (NMOSD) patients (n = 34), and healthy controls (HCs) (n = 34). T-cell profile analyses were performed by flow cytometry for MS patients and matched controls (n = 12). RESULTS: (1) The gut microbiome of MS patients was characterized by an increase of Streptococcus and a decrease of Prevotella_9; additionally, compared to NMOSD patients, Prevotella_9 was found to be much more abundant in MS patients. (2) A striking depletion of fecal acetate, propionate, and butyrate was observed in MS patients compared to HCs. (3) The abundance of Streptococcus was negatively correlated with the proportion of pTregs (P < 0.05) and positively correlated with Th17 cells (P < 0.05) in the peripheral blood, while the abundance of Prevotella_9 was negatively correlated with the Th17 cell frequency (P < 0.01), and the fecal SCFA level was positively correlated with pTreg frequency (P < 0.05). CONCLUSIONS: Gut dysbiosis and a lack of SCFAs exist in Chinese MS patients, which might be related to an aberrant immune response of MS; this relationship may have a diagnostic and therapeutic value for patients with MS.
BACKGROUND: Recent studies, mostly conducted in Western countries, showed that gut microbes are involved in the pathogenesis of multiple sclerosis (MS). OBJECTIVE: The aim of this study was to investigate whether gut dysbiosis is relevant to the initiation and progression of MS in a Chinese population. METHODS: Next-generation sequencing (NGS) and gas chromatography (GC) were integrated and used to compare the fecal bacterial communities and the short-chain fatty acid (SCFA) levels among relapsing-remitting MS (RRMS) patients (n = 34), neuromyelitis optica spectrum disorder (NMOSD) patients (n = 34), and healthy controls (HCs) (n = 34). T-cell profile analyses were performed by flow cytometry for MS patients and matched controls (n = 12). RESULTS: (1) The gut microbiome of MS patients was characterized by an increase of Streptococcus and a decrease of Prevotella_9; additionally, compared to NMOSD patients, Prevotella_9 was found to be much more abundant in MS patients. (2) A striking depletion of fecal acetate, propionate, and butyrate was observed in MS patients compared to HCs. (3) The abundance of Streptococcus was negatively correlated with the proportion of pTregs (P < 0.05) and positively correlated with Th17 cells (P < 0.05) in the peripheral blood, while the abundance of Prevotella_9 was negatively correlated with the Th17 cell frequency (P < 0.01), and the fecal SCFA level was positively correlated with pTreg frequency (P < 0.05). CONCLUSIONS: Gut dysbiosis and a lack of SCFAs exist in Chinese MS patients, which might be related to an aberrant immune response of MS; this relationship may have a diagnostic and therapeutic value for patients with MS.
Authors: Ali I Mirza; Feng Zhu; Natalie Knox; Jessica D Forbes; Gary Van Domselaar; Charles N Bernstein; Morag Graham; Ruth Ann Marrie; Janace Hart; E Ann Yeh; Douglas L Arnold; Amit Bar-Or; Julia O'Mahony; Yinshan Zhao; William Hsiao; Brenda Banwell; Emmanuelle Waubant; Helen Tremlett Journal: Neurology Date: 2021-12-22 Impact factor: 9.910
Authors: Felipe Papa Pellizoni; Aline Zazeri Leite; Nathália de Campos Rodrigues; Marcelo Jordão Ubaiz; Marina Ignácio Gonzaga; Nauyta Naomi Campos Takaoka; Vânia Sammartino Mariano; Wellington Pine Omori; Daniel Guariz Pinheiro; Euclides Matheucci Junior; Eleni Gomes; Gislane Lelis Vilela de Oliveira Journal: Int J Environ Res Public Health Date: 2021-04-27 Impact factor: 3.390