Literature DB >> 3109177

A novel method for the isolation of carboxypeptidase B.

E Dala, A Kiss, P Südi, B Szajáni.   

Abstract

Carboxypeptidase B was isolated from porcine pancreas by selective heat treatment of the autolyzed tissue at 60 degrees C and pH 6.0 the presence of phosphate ions. The heat treatment was followed by ammonium sulfate fractionation and ion-exchange in a batch system. The method could also be used for the isolation of carboxypeptidase B from beef pancreas.

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Year:  1986        PMID: 3109177

Source DB:  PubMed          Journal:  Acta Biochim Biophys Hung        ISSN: 0237-6261


  2 in total

1.  Effects of carbodiimide structure on the immobilization of enzymes.

Authors:  B Szajáni; P Südi; G Klamár; Z M Jászay; I Petneházy; L Töke
Journal:  Appl Biochem Biotechnol       Date:  1991-08       Impact factor: 2.926

2.  Preparation, characterization, and application of a novel immobilized carboxypeptidase B.

Authors:  P Südi; E Dala; B Szajáni
Journal:  Appl Biochem Biotechnol       Date:  1989-10       Impact factor: 2.926

  2 in total

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