Zhong-Bo Li1,2, Yi-Tian Fu1, Tian-Yin Cheng1,3, Guo-Min Yao2, Qiang-Hong Hou2, Fen Li1,3, Yu Zhao1,4, Feng-Cai Zou5, Guo-Hua Liu6,7. 1. Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan Province, People's Republic of China. 2. College of Animal Science and Technology, HuaiHua Vocational and Technical College, Huaihua, 418000, Hunan Province, People's Republic of China. 3. Hunan Co-Innovation Center of Animal Production Safety, Changsha, 410128, Hunan province, People's Republic of China. 4. College of Animal Science and Veterinary Medicine, Xinyang Agriculture and Forestry University, Xinyang, 464000, Henan Province, People's Republic of China. 5. College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, Yunnan Province, People's Republic of China. zfc1207@vip.163.com. 6. Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan Province, People's Republic of China. liuguohua5202008@163.com. 7. Hunan Co-Innovation Center of Animal Production Safety, Changsha, 410128, Hunan province, People's Republic of China. liuguohua5202008@163.com.
Abstract
INTRODUCTION: Haemaphysalis longicornis is an important ectoparasite of domestic and wild animals that can transmit many pathogens including viruses, fungi, bacteria and protozoa. MATERIALS AND METHODS: In this study, we examined genetic variation and population genetics in three mitochondrial (mt) genes [cox1 (cytochrome c subunit 1), rrnL (large subunit ribosomal RNA) and nad5 (NADH dehydrogenase 5)] among four H. longicornis populations from China. RESULTS: The sizes of the partial sequences of cox1, rrnL and nad5 were 776 bp, 409 bp, 510 bp, respectively. Among the obtained sequences, we identified 22 haplotypes for cox1, 2 haplotypes for rrnL and 17 haplotypes for nad5. Low gene flow and significant genetic differentiation (66.2%) were detected among H. longicornis populations. There was no rapid expansion event in the demographic history of four H. longicornis populations in China. In addition, phylogenetic analyses confirmed that all the Haemaphysalis isolates were H. longicornis which were segregated into two major clades. CONCLUSION: The mt DNA genes provide a potential novel genetic marker for molecular epidemiology of H. longicornis and assist in the control of tick and tick-borne diseases in humans and animals.
INTRODUCTION:Haemaphysalis longicornis is an important ectoparasite of domestic and wild animals that can transmit many pathogens including viruses, fungi, bacteria and protozoa. MATERIALS AND METHODS: In this study, we examined genetic variation and population genetics in three mitochondrial (mt) genes [cox1 (cytochrome c subunit 1), rrnL (large subunit ribosomal RNA) and nad5 (NADH dehydrogenase 5)] among four H. longicornis populations from China. RESULTS: The sizes of the partial sequences of cox1, rrnL and nad5 were 776 bp, 409 bp, 510 bp, respectively. Among the obtained sequences, we identified 22 haplotypes for cox1, 2 haplotypes for rrnL and 17 haplotypes for nad5. Low gene flow and significant genetic differentiation (66.2%) were detected among H. longicornis populations. There was no rapid expansion event in the demographic history of four H. longicornis populations in China. In addition, phylogenetic analyses confirmed that all the Haemaphysalis isolates were H. longicornis which were segregated into two major clades. CONCLUSION: The mt DNA genes provide a potential novel genetic marker for molecular epidemiology of H. longicornis and assist in the control of tick and tick-borne diseases in humans and animals.
Authors: Irina Muntaabski; Romina M Russo; María C Liendo; María A Palacio; Jorge L Cladera; Silvia B Lanzavecchia; Alejandra C Scannapieco Journal: Parasitol Res Date: 2020-01-08 Impact factor: 2.289