| Literature DB >> 310742 |
Abstract
Normal human T-lymphocytes can be induced to form colonies with a high cloning efficiency by seeding the cells directly in agar with normal human plasma and the lectins concanavalin A, phytohaemagglutinin, or pokeweed mitogen. The requirement for human plasma can be substituted, to different degrees with different lectins, by adding transferrin and albumin to foetal calf serum. This provides a useful system for identifying specific deficiencies in the requirements for normal T-lymphocyte colony formation.Entities:
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Year: 1978 PMID: 310742 PMCID: PMC1537431
Source DB: PubMed Journal: Clin Exp Immunol ISSN: 0009-9104 Impact factor: 4.330