Literature DB >> 310742

Control of cloning of normal human T lymphocytes by transferrin, albumin and different lectins.

L Sachs.   

Abstract

Normal human T-lymphocytes can be induced to form colonies with a high cloning efficiency by seeding the cells directly in agar with normal human plasma and the lectins concanavalin A, phytohaemagglutinin, or pokeweed mitogen. The requirement for human plasma can be substituted, to different degrees with different lectins, by adding transferrin and albumin to foetal calf serum. This provides a useful system for identifying specific deficiencies in the requirements for normal T-lymphocyte colony formation.

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Year:  1978        PMID: 310742      PMCID: PMC1537431     

Source DB:  PubMed          Journal:  Clin Exp Immunol        ISSN: 0009-9104            Impact factor:   4.330


  17 in total

1.  Partial replacement of serum by selenite, transferrin, albumin and lecithin in haemopoietic cell cultures.

Authors:  L J Guilbert; N N Iscove
Journal:  Nature       Date:  1976-10-14       Impact factor: 49.962

2.  Identification of albumin as the serum factor essential for the growth of activated human lymphocytes.

Authors:  H Spieker-Polet; H Polet
Journal:  J Biol Chem       Date:  1976-02-25       Impact factor: 5.157

3.  FORMATION OF PURE SUSPENSIONS OF MAST CELLS IN TISSUE CULTURE BY DIFFERENTIATION OF LYMPHOID CELLS FROM THE MOUSE THYMUS.

Authors:  H GINSBURG; L SACHS
Journal:  J Natl Cancer Inst       Date:  1963-07       Impact factor: 13.506

4.  Control of normal cell differentiation and the phenotypic reversion of malignancy in myeloid leukaemia.

Authors:  L Sachs
Journal:  Nature       Date:  1978-08-10       Impact factor: 49.962

5.  Colony formation by human T lymphocytes in agar medium.

Authors:  M H Claësson; M B Rodger; G R Johnson; S Whittingham; D Metcalf
Journal:  Clin Exp Immunol       Date:  1977-06       Impact factor: 4.330

6.  The growth of mouse bone marrow cells in vitro.

Authors:  T R Bradley; D Metcalf
Journal:  Aust J Exp Biol Med Sci       Date:  1966-06

7.  Identification of transferrin as a lymphocyte growth promoter in human serum.

Authors:  D C Tormey; R C Imrie; G C Mueller
Journal:  Exp Cell Res       Date:  1972-09       Impact factor: 3.905

8.  Regulation of the induction of colonies in vitro by normal human lymphocytes.

Authors:  E Gerassi; L Sachs
Journal:  Proc Natl Acad Sci U S A       Date:  1976-12       Impact factor: 11.205

9.  Growth of B-lymphocyte colonies in vitro.

Authors:  D Metcalf; G J Nossal; N L Warner; J F Miller; T E Mandel; J E Layton; G A Gutman
Journal:  J Exp Med       Date:  1975-12-01       Impact factor: 14.307

10.  Growth of B-lymphocytes clones in semisolid culture is mitogen dependent.

Authors:  P W Kincade; P Ralph; M A Moore
Journal:  J Exp Med       Date:  1976-05-01       Impact factor: 14.307

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  8 in total

1.  Difference in the cell proliferation and colony-forming ability of normal human T lymphocytes.

Authors:  S D Smith; L Sachs
Journal:  Clin Exp Immunol       Date:  1979-08       Impact factor: 4.330

2.  Differences in the formation of normal T lymphocyte colonies by peripheral blood cells from patients with chronic lymphocytic leukemia and Hodgkin's disease.

Authors:  D Douer; L Sachs
Journal:  Clin Exp Immunol       Date:  1979-12       Impact factor: 4.330

3.  Impaired in vitro growth of PHA induced T lymphocyte colonies in hemodialyzed renal failure patients.

Authors:  R Ghio; E Haupt; V Pistoia; A Perata; P Minale; M Ratti; P Boccaccio
Journal:  Blut       Date:  1985-03

4.  One-stage stimulation of human T-lymphocyte colony-forming units (TL-CFU) in a micro agar culture in glass capillaries.

Authors:  A J Ulmer; H D Flad
Journal:  Immunology       Date:  1979-10       Impact factor: 7.397

5.  T lymphocyte colony-forming capacity of patients with immunodeficiency diseases: relationship of colony formation to E rosette formation and lymphocyte proliferation.

Authors:  H G Herrod; W R Valenski
Journal:  Clin Exp Immunol       Date:  1981-09       Impact factor: 4.330

6.  The use of a T lymphocyte colony assay to measure mononuclear phagocyte function.

Authors:  M Y Gordon; J A King; E C Gordon-Smith
Journal:  Clin Exp Immunol       Date:  1980-10       Impact factor: 4.330

7.  Colony-forming lymphocytes in man. I. In vivo effect of hydrocortisone on phytohaemagglutinin-induced T cell colony formation.

Authors:  S Gupta; N Damle
Journal:  Clin Exp Immunol       Date:  1981-09       Impact factor: 4.330

8.  Transferrin synthesis by inducer T lymphocytes.

Authors:  J B Lum; A J Infante; D M Makker; F Yang; B H Bowman
Journal:  J Clin Invest       Date:  1986-03       Impact factor: 14.808

  8 in total

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