Literature DB >> 2610

Identification of albumin as the serum factor essential for the growth of activated human lymphocytes.

H Spieker-Polet, H Polet.   

Abstract

Albumin from human, bovine, or rabbit serum supported the growth of concanavalin A-stimulated human thymus-derived lymphocytes equally well. This activity was completely abolished by pepsin digestion. It was shown for bovine serum albumin that the albumin molecule itself, and neither an impurity nor a factor bound to albumin was essential for the growth of lymphocytes. This conclusion was based on observations that the growth-promoting activity could not be removed from albumin, and that the specific activity of albumin remained unaltered after the following procedures: molecular sieving at pH 7.5 at pH 3.0, and in 8 M urea at pH 6.6; ion exchange chromatography at pH 4.3 and in 8 M urea at pH 7.2; isoelectric focusing; charcoal treatment; acetone precipitation; and reduction with 2-mercaptoethanol in the presence of 8 M urea. Dimeric albumin was found to support growth of lymphocytes as well as monomeric albumin, and mercaptalbumin and non-mercaptalbumin were shown to have equal activity.

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Year:  1976        PMID: 2610

Source DB:  PubMed          Journal:  J Biol Chem        ISSN: 0021-9258            Impact factor:   5.157


  11 in total

1.  Increased glucocorticoid responsiveness of CD4+ T-cell clonal lines grown in serum-free media.

Authors:  D G Chilton; B H Johnson; L Danel-Moore; S Kawa; E B Thompson
Journal:  In Vitro Cell Dev Biol       Date:  1990-06

2.  An 18,000 molecular weight polypeptide induces early events and stimulates DNA synthesis in cultured cells.

Authors:  H R Bourne; E Rozengurt
Journal:  Proc Natl Acad Sci U S A       Date:  1976-12       Impact factor: 11.205

3.  Elucidation of an A and L system for amino acid transport in the human lymphoblast using a membrane filtration technique.

Authors:  R Bass; E Englesberg
Journal:  In Vitro       Date:  1979-10

4.  The binding of gitoxin to human plasma proteins.

Authors:  N Verbeke; P Pellegrin; A Vienne; M Lesne
Journal:  Eur J Clin Pharmacol       Date:  1979-11       Impact factor: 2.953

5.  Control of cloning of normal human T lymphocytes by transferrin, albumin and different lectins.

Authors:  L Sachs
Journal:  Clin Exp Immunol       Date:  1978-09       Impact factor: 4.330

6.  Separation of growth-stimulating activity of BSA fraction V from the bulk of albumin using Heparin Sepharose Chromatography.

Authors:  J Keenan; G Doherty; M Clynes
Journal:  Cytotechnology       Date:  1995-01       Impact factor: 2.058

7.  Deficiency in kwashiorkor serum of factors required for optimal lymphocyte transformation in vitro.

Authors:  D W Beatty; E B Dowdle
Journal:  Clin Exp Immunol       Date:  1979-03       Impact factor: 4.330

8.  Transferrin support of stimulated lymphocytes.

Authors:  W L Anderson; C G Chase; T B Tomasi
Journal:  In Vitro       Date:  1982-09

9.  1 alpha,25-dihydroxyvitamin D3 inhibits pokeweed mitogen-stimulated human B-cell activation: an analysis using serum-free culture conditions.

Authors:  K Shiozawa; S Shiozawa; S Shimizu; T Fujita
Journal:  Immunology       Date:  1985-09       Impact factor: 7.397

10.  Isolation and characterization of a distinct immunoregulatory isoform of alpha-fetoprotein produced by the normal fetus.

Authors:  N S van Oers; B L Cohen; R A Murgita
Journal:  J Exp Med       Date:  1989-09-01       Impact factor: 14.307

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