Nazila Alizadeh1, Milad Asadi1, Dariush Shanehbandi1, Venus Zafari2, Navid Shomali3, Touraj Asvadi4, Bita Sepehri5. 1. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. 2. Tuberculosis and Lung Disease Research Center, Tabriz University of Medical sciences, Tabriz, Iran. 3. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran. 4. Department of Surgery, Tabriz University of Medical sciences, Tabriz, Iran. 5. Liver and Gastrointestinal disease Research Center, Tabriz University of Medical sciences, Tabriz, Iran. bitasepehrii@yahoo.com.
Abstract
BACKGROUND: Genetic and epigenetic changes have strong role in the development of gastric cancer. The mutation of the MIR129-2 gene is one of the major causes in many cancers, especially gastric cancer. The aim of this study was to investigate the methylation changes of the MIR129-2 gene in tumor and normal tissue of patients with gastric cancer. METHOD: In this study, 50 gastric cancer patients with Iranian Azari ethnic origin without any familial relations were included. Genomic DNAs was extracted from the tumoral and normal tissues. Then the promotor regions of the MIR129-2 gene were analyzed by methylation-specific PCR (MSP) to evaluate the presence or absence of methylated CpG sites. RESULTS: There was a statistically significant difference in methylation level of MIR129-2 gene between tumoral and normal tissues. It was observed that 84 out of 100 CpG cites were methylated in tumoral tissues in compression to 13 out of 100 CpG cites in normal tissues. CONCLUSION: MIR129-2 gene was hypermethylated in tumoral tissues, suggesting that methylation is involved in the development of gastric cancer.
BACKGROUND: Genetic and epigenetic changes have strong role in the development of gastric cancer. The mutation of the MIR129-2 gene is one of the major causes in many cancers, especially gastric cancer. The aim of this study was to investigate the methylation changes of the MIR129-2 gene in tumor and normal tissue of patients with gastric cancer. METHOD: In this study, 50 gastric cancerpatients with Iranian Azari ethnic origin without any familial relations were included. Genomic DNAs was extracted from the tumoral and normal tissues. Then the promotor regions of the MIR129-2 gene were analyzed by methylation-specific PCR (MSP) to evaluate the presence or absence of methylated CpG sites. RESULTS: There was a statistically significant difference in methylation level of MIR129-2 gene between tumoral and normal tissues. It was observed that 84 out of 100 CpG cites were methylated in tumoral tissues in compression to 13 out of 100 CpG cites in normal tissues. CONCLUSION:MIR129-2 gene was hypermethylated in tumoral tissues, suggesting that methylation is involved in the development of gastric cancer.
Authors: Eva Bandres; Xabier Agirre; Nerea Bitarte; Natalia Ramirez; Ruth Zarate; Jose Roman-Gomez; Felipe Prosper; Jesus Garcia-Foncillas Journal: Int J Cancer Date: 2009-12-01 Impact factor: 7.396