The refractive index of the human cornea: A review.

The refractive index of the cornea and overlying tear film are key factors affecting refraction and overall optical properties of the eye. A figure of 1.376 is often quoted for the refractive index of the human cornea over the visible spectrum. In the 19th century estimates for the average refractive index of the human cornea ranged from 1.335 to 1.4391. Over the last two decades data obtained from either ex or in vivo corneas (under local anaesthesia with or without stromal resection) by contact Abbé refractometry show the refractive index of the cornea changes along its' depth undulating from around 1.400 at the epithelium to 1.380 at Bowman's layer, a low of 1.369 in the mid stroma and 1.373 at the endothelium. The mean refractive index of harvested tear samples is 1.337 rising to 1.482 for the overlying lipid layer. Contemporary measurements obtained in vivo by non-invasive methods reveal the average, or equivalent, refractive index of the tear film-cornea complex along the antero-posterior direction ranges from 1.423 to 1.436. Over the last 200 years calculations, with respect to the optics of the human eye, were based on values for the refractive index of the cornea obtained from invasive techniques. The refractive index of the cornea and overlying tear film appears to be higher than previously accepted and varies from case to case.