Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Stimulus-response coupling in a cell-free platelet membrane system. GTP-dependent release of Ca2+ by thrombin, and inhibition by pertussis toxin and a monoclonal antibody that blocks calcium release by IP3.

Literature DB >> 3106084

Stimulus-response coupling in a cell-free platelet membrane system. GTP-dependent release of Ca2+ by thrombin, and inhibition by pertussis toxin and a monoclonal antibody that blocks calcium release by IP3.

F O'Rourke, G B Zavoico, L H Smith, M B Feinstein.

Abstract

The Ca2+-mobilizing action of thrombin was demonstrated in a cell-free platelet membrane system consisting of open sheets of plasma membrane plus sealed membrane vesicles that accumulate Ca2+ and release Ca2+ in response to IP3. Thrombin plus GTP, acting on plasma membrane (not vesicles), produced a soluble factor (destroyed by alkaline phosphatase) that released Ca2+ from the vesicles. This effect of thrombin/GTP was blocked by a monoclonal antibody that binds to vesicles and prevents Ca2+ release by IP3. Pertussis toxin plus NAD ADP-ribosylated plasma membrane polypeptides of 39 and 41 kDa and blocked Ca2+ release by thrombin/GTP, but not by IP3.

Entities: Chemical Gene

Mesh：

Substances：

Year: 1987 PMID： 3106084 DOI： 10.1016/0014-5793(87)80037-6

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

Keyword Cloud
Cited

7 in total

1. The inositol 1,4,5-trisphosphate receptor binding sites of platelet membranes. pH-dependency, inhibition by polymeric sulphates, and the possible presence of arginine at the binding site.

Authors: F O'Rourke; M B Feinstein
Journal: Biochem J Date: 1990-04-15 Impact factor: 3.857

2. Activation of signal transduction in platelets by the tyrosine phosphatase inhibitor pervanadate (vanadyl hydroperoxide).

Authors: K M Pumiglia; L F Lau; C K Huang; S Burroughs; M B Feinstein
Journal: Biochem J Date: 1992-09-01 Impact factor: 3.857

3. Platelet adenylate cyclase and phospholipase C are affected differentially by ADP-ribosylation. Effects on thrombin-mediated responses.

Authors: H S Banga; R K Walker; L K Winberry; S E Rittenhouse
Journal: Biochem J Date: 1988-05-15 Impact factor: 3.857

4. Ca2+ release by inositol 1,4,5-trisphosphate is blocked by the K(+)-channel blockers apamin and tetrapentylammonium ion, and a monoclonal antibody to a 63 kDa membrane protein: reversal of blockade by K+ ionophores nigericin and valinomycin and purification of the 63 kDa antibody-binding protein.

Authors: F O'Rourke; K Soons; R Flaumenhauft; J Watras; C Baio-Larue; E Matthews; M B Feinstein
Journal: Biochem J Date: 1994-06-15 Impact factor: 3.857

Stimulus-response coupling in a cell-free platelet membrane system. GTP-dependent release of Ca2+ by thrombin, and inhibition by pertussis toxin and a monoclonal antibody that blocks calcium release by IP3.

1. The inositol 1,4,5-trisphosphate receptor binding sites of platelet membranes. pH-dependency, inhibition by polymeric sulphates, and the possible presence of arginine at the binding site.

2. Activation of signal transduction in platelets by the tyrosine phosphatase inhibitor pervanadate (vanadyl hydroperoxide).

3. Platelet adenylate cyclase and phospholipase C are affected differentially by ADP-ribosylation. Effects on thrombin-mediated responses.

4. Ca2+ release by inositol 1,4,5-trisphosphate is blocked by the K(+)-channel blockers apamin and tetrapentylammonium ion, and a monoclonal antibody to a 63 kDa membrane protein: reversal of blockade by K+ ionophores nigericin and valinomycin and purification of the 63 kDa antibody-binding protein.

5. How does fluoroaluminate activate human platelets?

Review 6. Arachidonic acid mobilization in platelets: the possible role of protein kinase C and G-proteins.

Review 7. Signal transduction mechanisms involved in hormonal Ca2+ fluxes.