M M Nascimento1, A J Alvarez2, X Huang3,4, S Hanway2,5, S Perry2,6, A Luce2,7, V P Richards8, R A Burne3. 1. 1 Division of Operative Dentistry, Department of Restorative Dental Sciences, College of Dentistry, University of Florida, Gainesville, FL, USA. 2. 2 Pediatric Dentistry Graduate Program, Department of Pediatric Dentistry, College of Dentistry, University of Florida, Gainesville, FL, USA. 3. 3 Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL, USA. 4. 4 Current affiliation: Division of General Dentistry, Eastman Institute for Oral Health, University of Rochester, Rochester, NY, USA. 5. 5 Current affiliation: Pediatric dentist, private practice, Tallahassee, FL, USA. 6. 6 Current affiliation: Pediatric dentist, private practice, St. Johns, FL, USA. 7. 7 Current affiliation: Pediatric dentist, private practice, St. Petersburg, FL, USA. 8. 8 Department of Biological Sciences, College of Sciences, Clemson University, Clemson, SC, USA.
Abstract
INTRODUCTION: Ammonia production via the arginine deiminase system (ADS) of oral bacteria can function to reduce the cariogenicity of oral biofilms by neutralizing glycolytic acids that cause tooth demineralization. OBJECTIVES: This cohort study investigated the relationship between ADS activity and bacterial profile changes of supragingival biofilms with caries experience among children over time. METHODS: A total of 79 children aged 2 to 7 y at baseline were assessed every 6 mo for a period of 18 mo. Children were grouped as caries free (CF), caries active with enamel lesions (CAE), or caries active with dentin lesions (CA). Supragingival plaque samples were collected from caries-free surfaces (PF) and from enamel (PE) and dentin (PD) lesions. Plaque ADS activity was measured by monitoring citrulline production from arginine and compared with ribosomal 16S rRNA-derived taxonomic profiles for the same samples. RESULTS: At baseline, 37% of the children were CF, 34% CAE, and 29% CA. At 18 mo, 26% were CF, 41% CAE, 23% CA, and 10% were caries experienced (new restorations but no caries activity). Throughout the study period, ADS activity was significantly higher in the CF group than the CA group (P < 0.0001), and ADS activity in the PF samples was significantly higher than in the PE and PD samples (P < 0.0001). Distance-based redundancy analysis showed that the bacterial communities could be differentiated when plaque samples are grouped into levels of high and low ADS activity. CONCLUSIONS: There is a positive correlation between caries activity and low arginolytic capacity of the supragingival oral biofilms of children and tooth surfaces over time. Measurements of arginine metabolism via ADS may be useful to differentiate the caries risk of individuals and tooth surfaces. KNOWLEDGE TRANSFER STATEMENT: Findings from this study support the development of new strategies for caries risk assessment and prevention based on modulation of the virulence of the oral microbiome through arginine metabolism in supragingival biofilms.
INTRODUCTION:Ammonia production via the arginine deiminase system (ADS) of oral bacteria can function to reduce the cariogenicity of oral biofilms by neutralizing glycolytic acids that cause tooth demineralization. OBJECTIVES: This cohort study investigated the relationship between ADS activity and bacterial profile changes of supragingival biofilms with caries experience among children over time. METHODS: A total of 79 children aged 2 to 7 y at baseline were assessed every 6 mo for a period of 18 mo. Children were grouped as caries free (CF), caries active with enamel lesions (CAE), or caries active with dentin lesions (CA). Supragingival plaque samples were collected from caries-free surfaces (PF) and from enamel (PE) and dentin (PD) lesions. Plaque ADS activity was measured by monitoring citrulline production from arginine and compared with ribosomal 16S rRNA-derived taxonomic profiles for the same samples. RESULTS: At baseline, 37% of the children were CF, 34% CAE, and 29% CA. At 18 mo, 26% were CF, 41% CAE, 23% CA, and 10% were caries experienced (new restorations but no caries activity). Throughout the study period, ADS activity was significantly higher in the CF group than the CA group (P < 0.0001), and ADS activity in the PF samples was significantly higher than in the PE and PD samples (P < 0.0001). Distance-based redundancy analysis showed that the bacterial communities could be differentiated when plaque samples are grouped into levels of high and low ADS activity. CONCLUSIONS: There is a positive correlation between caries activity and low arginolytic capacity of the supragingival oral biofilms of children and tooth surfaces over time. Measurements of arginine metabolism via ADS may be useful to differentiate the caries risk of individuals and tooth surfaces. KNOWLEDGE TRANSFER STATEMENT: Findings from this study support the development of new strategies for caries risk assessment and prevention based on modulation of the virulence of the oral microbiome through arginine metabolism in supragingival biofilms.
Authors: M M Nascimento; A J Alvarez; X Huang; C Browngardt; R Jenkins; M C Sinhoreti; A P D Ribeiro; D A Dilbone; V P Richards; T J Garrett; R A Burne Journal: J Dent Res Date: 2019-08-27 Impact factor: 6.116
Authors: Lauren M O'Connell; Ryan Santos; Garrett Springer; Robert A Burne; Marcelle M Nascimento; Vincent P Richards Journal: Appl Environ Microbiol Date: 2020-03-18 Impact factor: 4.792
Authors: Jonathon L Baker; James T Morton; Márcia Dinis; Ruth Alvarez; Nini C Tran; Rob Knight; Anna Edlund Journal: Genome Res Date: 2020-11-25 Impact factor: 9.043