| Literature DB >> 31030226 |
Zhiquan Liu1, Mao Chen1, Huanhuan Shan1, Siyu Chen1, Yuxin Xu1, Yuning Song1, Quanjun Zhang2, Hongming Yuan1, Hongsheng Ouyang1, Zhanjun Li3, Liangxue Lai4,5,6,7.
Abstract
Evolved xCas9(3.7) variant with broad PAM compatibility has been reported in cell lines, while its editing efficiency was site-specific. Here, we show that xCas9(3.7) can recognize a broad PAMs including NGG, NGA, and NGT, in both embryos and Founder (F0) rabbits. Furthermore, the codon-optimized xCas9-derived base editors, exBE4 and exABE, can dramatically improve the base editing efficiencies in rabbit embryos. Our results demonstrated that the optimized xCas9 with expanded PAM compatibility and enhanced base editing efficiency could be used for precise gene modifications in organisms.Entities:
Keywords: Base editors; CRISPR; PAM compatibility; xCas9
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Year: 2019 PMID： 31030226 DOI： 10.1007/s00018-019-03110-8
Source DB: PubMed Journal: Cell Mol Life Sci ISSN： 1420-682X Impact factor: 9.261