Literature DB >> 3102327

Selective regeneration of Bacillus subtilis protoplasts transformed to kanamycin resistance.

Z Jandová, P Tichý.   

Abstract

An HTY medium osmotically stabilized with 0.5 M D-glucitol was used for regeneration of Bacillus subtilis protoplasts. The application of glucitol as osmotic stabilizer allows simultaneous selection of cells resistant to kanamycin to be made since this antibiotic is not inactivated by glucitol when added to the regeneration medium.

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Year:  1987        PMID： 3102327     DOI： 10.1007/bf02877262

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  5 in total

1.  ENZYMICALLY AND PHYSICALLY INDUCED INHERITANCE CHANGES IN BACILLUS SUBTILIS.

Authors:  O E LANDMAN; S HALLE
Journal:  J Mol Biol       Date:  1963-12       Impact factor: 5.469

2.  Transformation in quasi spheroplasts of Bacillus subtilis.

Authors:  P Tichy; O E Landman
Journal:  J Bacteriol       Date:  1969-01       Impact factor: 3.490

3.  Fusion of protoplasts of Bacillus megaterium.

Authors:  K Fodor; L Alföldi
Journal:  Proc Natl Acad Sci U S A       Date:  1976-06       Impact factor: 11.205

4.  High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.

Authors:  S Chang; S N Cohen
Journal:  Mol Gen Genet       Date:  1979-01-05

5.  Isolation and characterization of cell wall-defective variants of Bacillus subtilis and Bacillus licheniformis.

Authors:  P B Wyrick; H J Rogers
Journal:  J Bacteriol       Date:  1973-10       Impact factor: 3.490
  5 in total
  1 in total

1.  Transformation of environmental Bacillus subtilis isolates by transiently inducing genetic competence.

Authors:  Reindert Nijland; J Grant Burgess; Jeff Errington; Jan-Willem Veening
Journal:  PLoS One       Date:  2010-03-16       Impact factor: 3.240
  1 in total

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