| Literature DB >> 31016693 |
Na An1, Aaron M Fleming1, Nicole C Rosecrans1, Yi Liao1, Cynthia J Burrows2.
Abstract
Formation of adducts to DNA is of great benefit to DNA sequencing and damage detection technology and to enzymology. Here we describe the synthesis and characterization procedures of 18-crown-6 adducts formed to abasic (AP) sites, 8-oxo-7,8-dihydro-2'-deoxyguanosine (OG), and 2'-deoxycytidine (C) residues in DNA oligodeoxynucleotides. These crown ether adducts were used as site-specific modifications to facilitate nanopore technology. The methods described can be readily expanded to attach other suitable primary amines of interest.Entities:
Keywords: 18-crown-6; 2′-Deoxycytidine; 8-Oxo-7,8-dihydro-2′-deoxyguanosine; Abasic sites; DNA adducts; DNA sequencing; Site-specific chemistry
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Year: 2019 PMID： 31016693 PMCID： PMC6482849 DOI： 10.1007/978-1-4939-9216-4_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745