| Literature DB >> 31012116 |
William J Wulftange1,2, Michelle A Rose1, Marcial Garmendia-Cedillos3, Davi da Silva1, Joanna E Poprawski2, Dhruv Srinivasachar2, Taylor Sullivan1, Langston Lim4, Valery V Bliskovsky5, Matthew D Hall6, Thomas J Pohida1, Robert W Robey2, Nicole Y Morgan1, Michael M Gottesman2.
Abstract
Commonly used monolayer cancer cell cultures fail to provide a physiologically relevant environment in terms of oxygen delivery. Here, we describe a three-dimensional (3D) bioreactor system where cancer cells are grown in Matrigel in modified six-well plates. Oxygen is delivered to the cultures through a polydimethylsiloxane (PDMS) membrane at the bottom of the wells, with microfabricated PDMS pillars to control oxygen delivery. The plates receive 3% oxygen from below and 0% oxygen at the top surface of the media, providing a gradient of 3-0% oxygen. We compared growth and transcriptional profiles for cancer cells grown in Matrigel in the bioreactor, 3D cultures grown in 21% oxygen, and cells grown in a standard hypoxia chamber at 3% oxygen. Additionally, we compared gene expression of conventional two-dimensional monolayer culture and 3D Matrigel culture in 21% oxygen. We conclude that controlled oxygen delivery may provide a more physiologically relevant 3D system. Published 2019. This article is a U.S. Government work and is in the public domain in the USA.Entities:
Keywords: 3D cell culture; RNA-seq; bioreactor; capillary oxygenation; oxygen gradient; transcriptome; tumor microenvironment
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Year: 2019 PMID： 31012116 PMCID： PMC6660365 DOI： 10.1002/jcp.28665
Source DB: PubMed Journal: J Cell Physiol ISSN： 0021-9541 Impact factor: 6.384