Literature DB >> 3099773

Ionophore A23187 induces a refractory state in thrombin-activated release of inositol phosphates.

G Moscat, F Moreno, S Iglesias, P Garcia-Barreno, A M Municio.   

Abstract

The phosphatidylinositol cycle has been proposed to be involved in the regulation of platelet functionality through the control of cytoplasmic Ca2+ levels. However, the requirements of phospholipase C for Ca2+ has not yet been elucidated in intact platelets. The primary purpose of the present study was to investigate the Ca2+ requirements of this enzyme in platelets from miniature swine by taking advantage of the permeabilizing properties of the ionophore A23187. Our results strongly suggest that the treatment of platelets with A23187 induces a refractory state in thrombin-stimulated release of inositol phosphates while 5-hydroxytryptamine (serotonin)-secretory capacity in response to thrombin remained constant. This refractory state seems to be dependent on some cytochalasin-inhibitable cytoskeletal phenomena.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3099773      PMCID： PMC1147195          DOI： 10.1042/bj2380709

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  34 in total

1.  Applications of metal buffers and metal indicators in biochemistry.

Authors:  J RAAFLAUB
Journal:  Methods Biochem Anal       Date:  1956

2.  Blood platelets express high levels of the pp60c-src-specific tyrosine kinase activity.

Authors:  A Golden; S P Nemeth; J S Brugge
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

3.  Phosphatidylinositol-specific phospholipase C activity of chromaffin granule-binding proteins.

Authors:  C E Creutz; L G Dowling; E M Kyger; R C Franson
Journal:  J Biol Chem       Date:  1985-06-25       Impact factor: 5.157

4.  Relationship between cytoplasmic free calcium and myosin light chain phosphorylation in intact platelets.

Authors:  T J Hallam; J L Daniel; J Kendrick-Jones; T J Rink
Journal:  Biochem J       Date:  1985-12-01       Impact factor: 3.857

5.  Thrombin induces serotonin secretion and aggregation independently of inositol phospholipids hydrolysis and protein phosphorylation in human platelets permeabilized with saponin.

Authors:  E G Lapetina; J Silió; M Ruggiero
Journal:  J Biol Chem       Date:  1985-06-10       Impact factor: 5.157

6.  Intracellular Ca2+ requirements for zymosan-stimulated phosphoinositide hydrolysis in mouse peritoneal macrophages.

Authors:  J Moscat; M Aracil; E Diez; J Balsinde; P Garcia Barreno; A M Municio
Journal:  Biochem Biophys Res Commun       Date:  1986-01-14       Impact factor: 3.575

7.  Metabolic and functional consequences of introducing inositol 1,4,5-trisphosphate into saponin-permeabilized human platelets.

Authors:  K S Authi; B J Evenden; N Crawford
Journal:  Biochem J       Date:  1986-02-01       Impact factor: 3.857

8.  Regulation of the association of membrane skeletal protein 4.1 with glycophorin by a polyphosphoinositide.

Authors:  R A Anderson; V T Marchesi
Journal:  Nature       Date:  1985 Nov 21-27       Impact factor: 49.962

9.  ATP depletion in human platelets caused by permeabilization with saponin does not prevent serotonin secretion induced by collagen.

Authors:  M Ruggiero; T P Zimmerman; E G Lapetina
Journal:  Biochem Biophys Res Commun       Date:  1985-09-16       Impact factor: 3.575

10.  A role for inositol triphosphate in intracellular Ca2+ mobilization and granule secretion in platelets.

Authors:  L F Brass; S K Joseph
Journal:  J Biol Chem       Date:  1985-12-05       Impact factor: 5.157
View more
  4 in total

1.  Phospholipase C-mediated hydrolysis of phosphatidylcholine is activated by muscarinic agonists.

Authors:  M T Diaz-Meco; P Larrodera; M Lopez-Barahona; M E Cornet; P G Barreno; J Moscat
Journal:  Biochem J       Date:  1989-10-01       Impact factor: 3.857

2.  Zymosan-induced release of inositol phosphates at resting cytosolic Ca2+ concentrations in macrophages.

Authors:  J Moscat; C Herrero; P Garcia-Barreno; A M Municio
Journal:  Biochem J       Date:  1987-03-01       Impact factor: 3.857

3.  Increased intracellular calcium mobilization in platelets from patients with type 2 (non-insulin-dependent) diabetes mellitus.

Authors:  H Ishii; F Umeda; T Hashimoto; H Nawata
Journal:  Diabetologia       Date:  1991-05       Impact factor: 10.122

4.  Mastoparan promotes exocytosis and increases intracellular cyclic AMP in human platelets. Evidence for the existence of a Ge-like mechanism of secretion.

Authors:  C P Wheeler-Jones; T Saermark; V V Kakkar; K S Authi
Journal:  Biochem J       Date:  1992-01-15       Impact factor: 3.857
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.