Ke Li1, Fan Hu2, Wan Xiong3, Qing Wei1, Fang-Fang Liu4. 1. Department of Blood Transfusion, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, P. R. China. 2. Department of Pathophysiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, P. R. China. 3. The Institute of Brain Research, Collaborative Innovation Center for Brain Science, Huazhong University of Science and Technology, 430030, Wuhan, P. R. China. 4. Department of Pathology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, 430014, Wuhan, P. R. China. 906463752@qq.com.
Abstract
PURPOSE: Heart disease is a major cause of mortality and disability worldwide. Melatonin is a neuroendocrine hormone and has been found to be protective in heart disease. However, the molecular basis underlying this cardioprotective effect is not fully understood. Here we aim to investigate melatonin-sensitive genes in cardiovascular system using public gene expression databases. METHODS: An innovative genomic analysis method, the weighted gene co-expression network analysis (WGCNA) combined with differential gene expression analysis, was used in this study. The algorithm was implemented in R/Bioconductor. RESULTS: Using this method, we provide a comprehensive characterization of transcriptional profiles associated with melatonin treatment. We found that 357 differentially expressed genes (DEGs) were highly sensitive to melatonin in mouse myocardium. Enrichment analysis showed that these 357 genes were mostly related to GO:0051984 (positive regulation of chromosome segregation), GO:0016605 (PML body) and GO:0006281 (DNA repair). We further obtained 5 hub genes from the 357 DEGs, including Set, Dhx40, Scaf11, Cfh, and Nup43. CONCLUSIONS: We identified numerous melatonin-sensitive genes and further identified five hub genes. The five novel genes are possibly associated with the myocardial benefits of melatonin.
PURPOSE:Heart disease is a major cause of mortality and disability worldwide. Melatonin is a neuroendocrine hormone and has been found to be protective in heart disease. However, the molecular basis underlying this cardioprotective effect is not fully understood. Here we aim to investigate melatonin-sensitive genes in cardiovascular system using public gene expression databases. METHODS: An innovative genomic analysis method, the weighted gene co-expression network analysis (WGCNA) combined with differential gene expression analysis, was used in this study. The algorithm was implemented in R/Bioconductor. RESULTS: Using this method, we provide a comprehensive characterization of transcriptional profiles associated with melatonin treatment. We found that 357 differentially expressed genes (DEGs) were highly sensitive to melatonin in mouse myocardium. Enrichment analysis showed that these 357 genes were mostly related to GO:0051984 (positive regulation of chromosome segregation), GO:0016605 (PML body) and GO:0006281 (DNA repair). We further obtained 5 hub genes from the 357 DEGs, including Set, Dhx40, Scaf11, Cfh, and Nup43. CONCLUSIONS: We identified numerous melatonin-sensitive genes and further identified five hub genes. The five novel genes are possibly associated with the myocardial benefits of melatonin.
Authors: Gaia Favero; Lorenzo Franceschetti; Barbara Buffoli; Mohammed H Moghadasian; Russel J Reiter; Luigi F Rodella; Rita Rezzani Journal: Ageing Res Rev Date: 2016-11-22 Impact factor: 10.895