Jyoti Mishra1, Seerat Talwar1, Lovejeet Kaur2, Ketaki Chandiok1, Suniti Yadav1, Manju Puri3, M P Sachdeva1, R Lakshmy4, K N Saraswathy5. 1. Department of Anthropology, University of Delhi, Delhi, India. 2. Centre for Cellular and Molecular Biology, Hyderabad, India. 3. Department of Obstetrics and Gynaecology, Lady Hardinge Medical College, Delhi, India. 4. Department of Cardiac Biochemistry, AIIMS, Delhi, India. 5. Department of Anthropology, University of Delhi, Delhi, India. Electronic address: knsaraswathy@yahoo.com.
Abstract
AIM: The purpose of the present study is to evaluate and understand the association of global and MTHFR gene specific methylation in preeclampsia and recurrent miscarriages in light of MTHFR C677T polymorphism. METHODS: The subjects comprised of recurrent miscarriage cases, their gestation matched controls, preeclampsia cases and matched controls. A set of women at full term were also recruited. Fasting blood sample (~5 ml) was drawn from all the participants followed by DNA extraction, global DNA methylation and MTHFR gene specific methylation. MTHFR C677T polymorphism was analysed by PCR followed by RFLP. RESULTS HIGHER: Global DNA methylation at maternal front (p = 0.04) and hypomethylation of MTHFR gene at fetal front (p = 0.001) might be a characteristic of preeclampsia. Recurrent miscarriage cases were having significantly (p = 0.002) hyper MTHFR gene specific methylation as compared to controls. Women carrying CT genotype were found to be having significantly (p = 0.001) higher global DNA methylation in PE cases and MTHFR gene specific methylation (p = 0.005) in RM cases. Intergenerational analysis revealed similar patterns of global DNA methylation and MTHFR gene specific methylation among both PE and RM cases at maternal and fetal fronts. CONCLUSION: The study highlights the importance of global DNA methylation in Preeclampsia and MTHFR gene specific methylation in recurrent miscarriages. MTHFR C677T gene polymorphism in association with global and gene specific methylation seem to play a pivotal role in PE and RM respectively.
AIM: The purpose of the present study is to evaluate and understand the association of global and MTHFR gene specific methylation in preeclampsia and recurrent miscarriages in light of MTHFRC677T polymorphism. METHODS: The subjects comprised of recurrent miscarriage cases, their gestation matched controls, preeclampsia cases and matched controls. A set of women at full term were also recruited. Fasting blood sample (~5 ml) was drawn from all the participants followed by DNA extraction, global DNA methylation and MTHFR gene specific methylation. MTHFRC677T polymorphism was analysed by PCR followed by RFLP. RESULTS HIGHER: Global DNA methylation at maternal front (p = 0.04) and hypomethylation of MTHFR gene at fetal front (p = 0.001) might be a characteristic of preeclampsia. Recurrent miscarriage cases were having significantly (p = 0.002) hyper MTHFR gene specific methylation as compared to controls. Women carrying CT genotype were found to be having significantly (p = 0.001) higher global DNA methylation in PE cases and MTHFR gene specific methylation (p = 0.005) in RM cases. Intergenerational analysis revealed similar patterns of global DNA methylation and MTHFR gene specific methylation among both PE and RM cases at maternal and fetal fronts. CONCLUSION: The study highlights the importance of global DNA methylation in Preeclampsia and MTHFR gene specific methylation in recurrent miscarriages. MTHFRC677T gene polymorphism in association with global and gene specific methylation seem to play a pivotal role in PE and RM respectively.
Authors: Paula Benny; Kelly Yamasato; Breck Yunits; Xun Zhu; Travers Ching; Lana X Garmire; Marla J Berry; Dena Towner Journal: PLoS One Date: 2019-09-26 Impact factor: 3.240
Authors: Tainá Gomes Diniz; Alexandre Sérgio Silva; Mayara Karla Dos Santos Nunes; Mateus Duarte Ribeiro; João Modesto Filho; Rayner Anderson Ferreira do Nascimento; Cecília Neta Alves Pegado Gomes; Isabella Wanderley de Queiroga Evangelista; Naila Francis Paulo de Oliveira; Darlene Camati Persuhn Journal: Front Physiol Date: 2021-01-12 Impact factor: 4.566