Tainá Gomes Diniz1, Alexandre Sérgio Silva2, Mayara Karla Dos Santos Nunes3, Mateus Duarte Ribeiro4, João Modesto Filho5, Rayner Anderson Ferreira do Nascimento6, Cecília Neta Alves Pegado Gomes7, Isabella Wanderley de Queiroga Evangelista8, Naila Francis Paulo de Oliveira9, Darlene Camati Persuhn10. 1. Post-Graduate Program in Nutrition Science, Federal University of Paraiba, Joao Pessoa, Brazil. 2. Department of Physical Education, Federal University of Paraiba (UFPB), Joao Pessoa, Brazil. 3. Post-Graduation Program in Development and Technological Innovation of Medicines (DITM), Federal University of Paraiba, Joao Pessoa, Brazil. 4. Post-Graduation Program in Physical Education, Federal University of Paraiba, Joao Pessoa, Brazil. 5. Department of Internal Medicine, Federal University of Paraiba, Joao Pessoa, Brazil. 6. Uninassau Faculty, Mauricio de Nassau University, João Pessoa, Brazil. 7. Nephrology Clinic, Lauro Wanderley University Hospital, Federal University of Paraiba, Joao Pessoa, Brazil. 8. Ophthalmology Reference Center, Lauro Wanderley University Hospital, Federal University of Paraiba, Joao Pessoa, Brazil. 9. Department of Molecular Biology, Federal University of Paraiba, Joao Pessoa, Brazil. 10. Department of Molecular Biology and Post-Graduation Program in Nutrition Science, Federal University of Paraiba, Joao Pessoa, Brazil.
Abstract
INTRODUCTION: MTHFR methylation status is associated with microvascular complications in diabetes, but the factors influencing this profile remain unknown. OBJECTIVE: The aim of this study was to evaluate the influence of physical activity level and nutritional status on the methylation profile of the MTHFR gene in patients with type 2 diabetes mellitus (T2DM). METHODS: A total of 111 patients, 43 men and 68 women diagnosed with DM (7.0 ± 2.3 years), answered the International Physical Activity Questionnaire (IPAQ) and underwent blood collection for biochemical analysis, DNA extraction, and MTHFR gene methylation profile determination. RESULT: The comparison of the methylation pattern showed that the partially methylated profile predominates in the insufficiently active group (85%), which does not occur in the sufficiently active group (54%) (p = 0.012). No differences were found in the nutritional status comparison. Logistic regression including overweight, waist circumference, gender, age, time of DM, hypertension, dyslipidemia, smoking, alcoholism, and family DM revealed that the association of the level of physical activity with methylation profile proved to be independent of these confounding variables. Considering the partially methylated profile as a result, being physically inactive favors the partially methylated MTHFR pattern in patients with DM. CONCLUSION: We concluded that insufficient physical activity is associated with partially methylated pattern of MTHFR promoter.
INTRODUCTION: MTHFR methylation status is associated with microvascular complications in diabetes, but the factors influencing this profile remain unknown. OBJECTIVE: The aim of this study was to evaluate the influence of physical activity level and nutritional status on the methylation profile of the MTHFR gene in patients with type 2 diabetes mellitus (T2DM). METHODS: A total of 111 patients, 43 men and 68 women diagnosed with DM (7.0 ± 2.3 years), answered the International Physical Activity Questionnaire (IPAQ) and underwent blood collection for biochemical analysis, DNA extraction, and MTHFR gene methylation profile determination. RESULT: The comparison of the methylation pattern showed that the partially methylated profile predominates in the insufficiently active group (85%), which does not occur in the sufficiently active group (54%) (p = 0.012). No differences were found in the nutritional status comparison. Logistic regression including overweight, waist circumference, gender, age, time of DM, hypertension, dyslipidemia, smoking, alcoholism, and family DM revealed that the association of the level of physical activity with methylation profile proved to be independent of these confounding variables. Considering the partially methylated profile as a result, being physically inactive favors the partially methylated MTHFR pattern in patients with DM. CONCLUSION: We concluded that insufficient physical activity is associated with partially methylated pattern of MTHFR promoter.
Authors: Andrei C Sposito; Bruno Caramelli; Francisco A H Fonseca; Marcelo C Bertolami; Abrahão Afiune Neto; Aguinaldo David Souza; Ana Maria Pitta Lottenberg; Ana Paula Chacra; André A Faludi; Andréia A Loures-Vale; Antônio Carlos Carvalho; Bruce Duncan; Bruno Gelonese; Carisi Polanczyk; Carlos Roberto M Rodrigues Sobrinho; Carlos Scherr; Cynthia Karla; Dikran Armaganijan; Emílio Moriguchi; Francisco Saraiva; Geraldo Pichetti; Hermes Toros Xavier; Hilton Chaves; Jairo Lins Borges; Jayme Diament; Jorge Ilha Guimarães; José Carlos Nicolau; José Ernesto dos Santos; José Jayme Galvão de Lima; José Luiz Vieira; José Paulo Novazzi; José Rocha Faria Neto; Kerginaldo P Torres; Leonor de Almeida Pinto; Liliana Bricarello; Luiz Carlos Bodanese; Luiz Introcaso; Marcus Vinícius Bolívar Malachias; Maria Cristina Izar; Maria Eliane C Magalhães; Maria Inês Schmidt; Mariléia Scartezini; Moacir Nobre; Murilo Foppa; Neusa A Forti; Otávio Berwanger; Otávio C E Gebara; Otávio Rizzi Coelho; Raul C Maranhão; Raul Dias dos Santos Filho; Rosana Perim Costa; Sandhi Barreto; Sérgio Kaiser; Silvia Ihara; Tales de Carvalho; Tania Leme Rocha Martinez; Waldir Gabriel Miranda Relvas; Wilson Salgado Journal: Arq Bras Cardiol Date: 2007-04 Impact factor: 2.000
Authors: Ronald E Gangnon; Matthew D Davis; Larry D Hubbard; Lloyd M Aiello; Emily Y Chew; Frederick L Ferris; Marian R Fisher Journal: Invest Ophthalmol Vis Sci Date: 2008-06-06 Impact factor: 4.799
Authors: Catherine Duggan; Ming Yu; Amber R Willbanks; Jean de Dieu Tapsoba; Ching-Yun Wang; William M Grady; Anne McTiernan Journal: Epigenetics Date: 2021-10-06 Impact factor: 4.861
Authors: Laura Bordoni; Irene Petracci; Monika Mlodzik-Czyzewska; Anna M Malinowska; Artur Szwengiel; Marcin Sadowski; Rosita Gabbianelli; Agata Chmurzynska Journal: Oxid Med Cell Longev Date: 2022-01-29 Impact factor: 6.543