| Literature DB >> 30978341 |
Shan He1, Ziming Li2, Yongfeng Yu2, Qingyu Zeng3, Yirui Cheng3, Wenxiang Ji2, Weiliang Xia4, Shun Lu5.
Abstract
Tumor-derived exosomes contain informative microRNAs involved in carcinogenesis, cell migration, invasion and epithelial-mesenchymal transition (EMT), eventually contributing to metastasis of cancers. This study aims to clarify which and how exosomal miRNA affects tumor carcinogenesis and metastasis. Among them, miR-499a-5p was upregulated in both highly metastatic lung cancer cell line and their exosomes. MiR-499a-5p overexpression promoted cell proliferation, migration and EMT, while miR-499a-5p knockdown suppressed these processes in vitro. Inhibition of miR-499a-5p by antagomirs administration restrained tumor growth in vivo. Consequently, miR499a-sufficient exosomes, derived from highly metastatic cell line, enhanced cell proliferation, migration and EMT via mTOR pathway, and the effect could be inhibited by miR-499a-5p inhibitor. The study reveals the potential diagnostic and therapeutic value of cancer-derived exosomal miR-499a-5p, and sheds a new insight on a novel molecular mechanism which modulates metastasis.Entities:
Keywords: Exosome; Lung cancer; mTOR pathway; miR499a
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Year: 2019 PMID： 30978341 DOI： 10.1016/j.yexcr.2019.03.035
Source DB: PubMed Journal: Exp Cell Res ISSN： 0014-4827 Impact factor: 3.905