| Literature DB >> 30974396 |
Dan Liu1, Shuai Meng2, Zhonghuai Xiang3, Ningjia He4, Guangwei Yang5.
Abstract
Herein, five polyphenol oxidases (PPOs) obtained from Morus notabilis (Mn) were characterized. Chlorogenic acid was the most readily oxidized substrate by these MnPPOs, and the products derived from the oxidation of chlorogenic acid by MnPPOs were tested for antimicrobial activity. The results showed that products of the five MnPPOs exhibited good inhibitory effects against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Sclerotinia sclerotiorum, and Botrytis cinerea. Because the products of MnPPO1 exhibited the strongest antimicrobial activity, the antimicrobial mechanism of these products was explored. The results showed that the products of MnPPO1 increased cell membrane permeability and chitinase and β-1,3-glucanase activities.Entities:
Keywords: Antimicrobial activities; Chlorogenic acid; ELISA; Moraceae polyphenol oxidase; Morus notabilis
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Year: 2019 PMID： 30974396 DOI： 10.1016/j.phytochem.2019.03.026
Source DB: PubMed Journal: Phytochemistry ISSN： 0031-9422 Impact factor: 4.072