Literature DB >> 30971435

Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production.

Douglas F Browning1, Rita E Godfrey2, Kirsty L Richards3, Colin Robinson3, Stephen J W Busby1.   

Abstract

The Escherichia coli lac operon promoter is widely used as a tool to control recombinant protein production in bacteria. Here, we give a brief review of how it functions, how it is regulated, and how, based on this knowledge, a suite of lac promoter derivatives has been developed to give a controlled expression that is suitable for diverse biotechnology applications.
© 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.

Entities:  

Keywords:  Escherichia coli; bacterial transcription initiation; lac promoter; lac repressor; recombinant protein production; tac promoter

Mesh：

Substances：

Year:  2019        PMID： 30971435     DOI： 10.1042/BST20190059

Source DB:  PubMed          Journal:  Biochem Soc Trans        ISSN： 0300-5127            Impact factor:   5.407


  6 in total

1.  Combinatorial strategy towards the efficient expression of lipoxygenase in Escherichia coli at elevated temperatures.

Authors:  Cuiping Pang; Song Liu; Guoqiang Zhang; Jingwen Zhou; Guocheng Du; Jianghua Li
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2.  Activation by NarL at the Escherichia coli ogt promoter.

Authors:  Patcharawarin Ruanto; David L Chismon; Joanne Hothersall; Rita E Godfrey; David J Lee; Stephen J W Busby; Douglas F Browning
Journal:  Biochem J       Date:  2020-08-14       Impact factor: 3.857

3.  Multiplexed characterization of rationally designed promoter architectures deconstructs combinatorial logic for IPTG-inducible systems.

Authors:  Timothy C Yu; Winnie L Liu; Marcia S Brinck; Jessica E Davis; Jeremy Shek; Grace Bower; Tal Einav; Kimberly D Insigne; Rob Phillips; Sriram Kosuri; Guillaume Urtecho
Journal:  Nat Commun       Date:  2021-01-12       Impact factor: 14.919

Review 4.  Challenges Associated With the Formation of Recombinant Protein Inclusion Bodies in Escherichia coli and Strategies to Address Them for Industrial Applications.

Authors:  Arshpreet Bhatwa; Weijun Wang; Yousef I Hassan; Nadine Abraham; Xiu-Zhen Li; Ting Zhou
Journal:  Front Bioeng Biotechnol       Date:  2021-02-10

5.  Development of a novel expression system in lactic acid bacteria controlled by a broad-host-range promoter PsrfA.

Authors:  Chengran Guan; Yuan Yuan; Yan Ma; Xin Wang; Chenchen Zhang; Maolin Lu; Ruixia Gu; Dawei Chen
Journal:  Microb Cell Fact       Date:  2022-02-15       Impact factor: 5.328

6.  Inexpensive protein overexpression driven by the NarL transcription activator protein.

Authors:  Joanne Hothersall; Sandie Lai; Nan Zhang; Rita E Godfrey; Patcharawarin Ruanto; Sarah Bischoff; Colin Robinson; Tim W Overton; Stephen J W Busby; Douglas F Browning
Journal:  Biotechnol Bioeng       Date:  2022-03-06       Impact factor: 4.395
  6 in total

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