Mari C W Myhrstad1, Vanessa D de Mello2, Ingrid Dahlman3, Marjukka Kolehmainen2, Jussi Paananen2, Amanda Rundblad4, Carsten Carlberg5, Ole Kristoffer Olstad6, Jussi Pihlajamäki2,7, Kirsten B Holven4,8, Kjeld Hermansen9, Lars O Dragsted10, Ingibjörg Gunnarsdottir11, Lieselotte Cloetens12, Matilda Ulmius Storm12, Björn Åkesson12,13, Fredrik Rosqvist14, Janne Hukkanen15, Karl-Heinz Herzig16,17, Ulf Risérus14, Inga Thorsdottir11, Kaisa S Poutanen2,18, Markku J Savolainen15, Ursula Schwab2,7, Peter Arner3, Matti Uusitupa2, Stine M Ulven4. 1. Department of Nursing and Health Promotion, Faculty of Health Sciences, Oslo Metropolitan University, 0130, Oslo, Norway. 2. Institute of Public Health and Clinical Nutrition, University of Eastern Finland, 70211, Kuopio, Finland. 3. Department of Medicine (H7), Karolinska Institute, 141 86, Stockholm, Sweden. 4. Department of Nutrition, Institute for Basic Medical Sciences, University of Oslo, 0316, Oslo, Norway. 5. Institute of Biomedicine, University of Eastern Finland, 70211, Kuopio, Finland. 6. Department of Medical Biochemistry, Oslo University Hospital, 0424, Oslo, Norway. 7. Department of Medicine, Endocrinology and Clinical Nutrition, Kuopio University Hospital, 70029, Kuopio, Finland. 8. Norwegian National Advisory Unit on Familial Hypercholesterolemia, Department of Endocrinology, Morbid Obesity and Preventive Medicine, Oslo University Hospital, 0424, Oslo, Norway. 9. Department of Endocrinology and Internal Medicine, Aarhus University Hospital, 8200, Aarhus, Denmark. 10. Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, DK-2200 Copenhagen N, Denmark. 11. Unit for Nutrition Research, University of Iceland and Landspitali - The National University Hospital of Iceland, 101, Reykjavík, Iceland. 12. Biomedical Nutrition, Pure and Applied Biochemistry, Lund University, 221 00, Lund, Sweden. 13. Department of Clinical Nutrition, Skåne University Hospital, 221 00, Lund, Sweden. 14. Department of Public Health and Caring Sciences, Clinical Nutrition and Metabolism, Uppsala University, 751 22, Uppsala, Sweden. 15. Department of Internal Medicine and Biocenter Oulu, University of Oulu, and Medical Research Center, Oulu University Hospital, 90014, Oulu, Finland. 16. Institute of Biomedicine and Biocenter of Oulu, University of Oulu, Medical Research Center (MRC) and University Hospital, 90014, Oulu, Finland. 17. Department of Gastroenterology and Metabolism, Poznań University of Medical Sciences, 10 61-701, Poznań, Poland. 18. VTT Technical Research Centre of Finland, 02044 VTT, Espoo, Finland.
Abstract
SCOPE: To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. METHODS AND RESULTS: Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing anisocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p-value < 0.05). Twenty-five of these are significantly regulated (FDR q-value < 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF-κB, are downregulated in the healthy Nordic diet group. CONCLUSION: These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation.
RCT Entities:
SCOPE: To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. METHODS AND RESULTS: Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p-value < 0.05). Twenty-five of these are significantly regulated (FDR q-value < 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF-κB, are downregulated in the healthy Nordic diet group. CONCLUSION: These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation.
Authors: Stine M Ulven; Kirsten B Holven; Amanda Rundblad; Mari C W Myhrstad; Lena Leder; Ingrid Dahlman; Vanessa D de Mello; Ursula Schwab; Carsten Carlberg; Jussi Pihlajamäki; Kjeld Hermansen; Lars O Dragsted; Ingibjörg Gunnarsdottir; Lieselotte Cloetens; Björn Åkesson; Fredrik Rosqvist; Janne Hukkanen; Karl-Heinz Herzig; Markku J Savolainen; Ulf Risérus; Inga Thorsdottir; Kaisa S Poutanen; Peter Arner; Matti Uusitupa; Marjukka Kolehmainen Journal: Nutrients Date: 2019-12-03 Impact factor: 5.717