Development and characterization of biocomposite films made from kefiran, carboxymethyl cellulose and Satureja Khuzestanica essential oil.

Kefiran-carboxymethyl cellulose biocomposite films incorporated with Satureja Khuzestanica essential oil were developed and characterized. Results indicated that increase in the concentration of the essential oil increased ultimate tensile strength and contact angle but decreased elongation at break, moisture content and water vapor permeability. It also significantly altered color parameters and the percentage of light transmission in the visible and ultraviolet range. Fourier transform infrared spectroscopy revealed the formation of hydrogen bonds between polymer matrix and essential oil. Scanning electron microscopy showed that the surface structure of the films was homogeneous without porosity. Increase in storage modulus and glass transmission temperature in films incorporated with the essential oil was observed through dynamic mechanical thermal analysis. Moreover, significant increase in antioxidant properties and phenolic compounds were noticed. Ultimately, results obtained from evaluation of antimicrobial characteristics of films indicated their inhibitory effects against Staphylococcus aureus and Escherichia coli bacteria.