| Literature DB >> 30949960 |
Qiuyan Zhang1,2, Na Li1,2, Chenglin Deng1, Zherui Zhang1,2, Xiaodan Li1, Kentaro Yoshii3, Hanqing Ye1, Bo Zhang4.
Abstract
Omsk hemorrhagic fever virus (OHFV) is a tick-borne flavivirus classified as a biosafety level-4 (BSL4) pathogen. Studies of OHFV are restricted to be conducted within BSL4 laboratories. Currently, no commercial vaccines or antiviral drugs are available against OHFV infection. In this study, we recovered a replication-deficient OHFV with an NS1 deletion (OHFV-ΔNS1) and reporter virus replacing NS1 with the Gaussia luciferase (Gluc) (OHFV-ΔNS1-Gluc). Both the defective OHFV-ΔNS1 and OHFV-ΔNS1-Gluc virus could only replicate efficiently in the BHK21 cell line expressing NS1 (BHK21NS1) but not in naïve BHK21 cells. The Gluc reporter gene of OHFV-ΔNS1-Gluc virus was maintained stably after serial passaging of BHK21NS1 cells and was used to surrogate the replication of OHFV. Using NITD008, OHFV-ΔNS1-Gluc virus was validated for antiviral screening, and high-throughput screening parameters were optimized in a 96-well plate format with a calculated Z' value above 0.5. The OHFV-ΔNS1-Gluc reporter virus is a powerful tool for antiviral screening as well as viral replication and pathogenesis studies in BSL2 laboratories.Entities:
Keywords: Antiviral screening; Gaussia luciferase (Gluc); NS1; Omsk hemorrhagic fever virus (OHFV); Trans complementation
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Year: 2019 PMID： 30949960 PMCID： PMC6687815 DOI： 10.1007/s12250-019-00109-0
Source DB: PubMed Journal: Virol Sin ISSN： 1995-820X Impact factor: 4.327