Literature DB >> 15168210

Sub-genomic replicons of Tick-borne encephalitis virus.

D Hayasaka1, K Yoshii, T Ueki, T Iwasaki, I Takashima.   

Abstract

We constructed three sub-genomic replicons of Tick-borne encephalitis virus (TBEV) (Oshima REP, Oshima REP-GFP and Oshima REP-Neo) by deleting genes coding for structural proteins without or with insertion of green fluorescent protein (GFP) or Neo genes, respectively. BHK cells transfected with Oshima REP expressed the viral non-structural antigens in immunofluorescent and western blot analyses. GFP and viral antigens were co-expressed in the transfected cells with Oshima REP-GFP. G418-resistant cells harboring Oshima REP-Neo consistently expressed the antigens without showing any apparent CPE. These replicons constructed in this study will be useful in studies on the replication, assembly and packaging of TBEV, and to develop vaccines and gene-delivering systems.

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Year:  2004        PMID： 15168210     DOI： 10.1007/s00705-003-0262-y

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  8 in total

1.  Construction and mutagenesis of an artificial bicistronic tick-borne encephalitis virus genome reveals an essential function of the second transmembrane region of protein e in flavivirus assembly.

Authors:  Klaus K Orlinger; Verena M Hoenninger; Regina M Kofler; Christian W Mandl
Journal:  J Virol       Date:  2006-10-11       Impact factor: 5.103

2.  Temperature-dependent production of pseudoinfectious dengue reporter virus particles by complementation.

Authors:  Camilo Ansarah-Sobrinho; Steevenson Nelson; Christiane A Jost; Stephen S Whitehead; Theodore C Pierson
Journal:  Virology       Date:  2008-09-17       Impact factor: 3.616

3.  Trans Complementation of Replication-defective Omsk Hemorrhagic Fever Virus for Antiviral Study.

Authors:  Qiuyan Zhang; Na Li; Chenglin Deng; Zherui Zhang; Xiaodan Li; Kentaro Yoshii; Hanqing Ye; Bo Zhang
Journal:  Virol Sin       Date:  2019-04-04       Impact factor: 4.327

4.  Selection and analysis of mutations in an encephalomyocarditis virus internal ribosome entry site that improve the efficiency of a bicistronic flavivirus construct.

Authors:  Klaus K Orlinger; Regina M Kofler; Franz X Heinz; Verena M Hoenninger; Christian W Mandl
Journal:  J Virol       Date:  2007-09-12       Impact factor: 5.103

5.  Viral replicons as valuable tools for drug discovery.

Authors:  Holger Hannemann
Journal:  Drug Discov Today       Date:  2020-04-06       Impact factor: 7.851

Review 6.  RNA Viruses as Tools in Gene Therapy and Vaccine Development.

Authors:  Kenneth Lundstrom
Journal:  Genes (Basel)       Date:  2019-03-01       Impact factor: 4.096

Review 7.  Replicon RNA Viral Vectors as Vaccines.

Authors:  Kenneth Lundstrom
Journal:  Vaccines (Basel)       Date:  2016-11-07

8.  Microscopic Visualisation of Zoonotic Arbovirus Replication in Tick Cell and Organ Cultures Using Semliki Forest Virus Reporter Systems.

Authors:  Lesley Bell-Sakyi; Sabine Weisheit; Claudia Rückert; Gerald Barry; John Fazakerley; Rennos Fragkoudis
Journal:  Vet Sci       Date:  2016-09-29
  8 in total

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