Xingwei Zheng1, Xiaojie Wen2, Ling Qiao1, Jiajia Zhao1, Xiaojun Zhang3, Xin Li3, Shuwei Zhang3, Zujun Yang3, Zhijian Chang3, Jianli Chen4, Jun Zheng5. 1. Institute of Wheat Research, Shanxi Academy of Agricultural Sciences, Linfen, China. 2. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China. 3. The Shanxi Province Key Laboratory of Crop Genetics and Gene Improvement, Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi, China. 4. Department of Plant Sciences, University of Idaho, Aberdeen, ID, USA. jchen@uidaho.edu. 5. Institute of Wheat Research, Shanxi Academy of Agricultural Sciences, Linfen, China. zhengjun@sxagri.ac.cn.
Abstract
MAIN CONCLUSION: In wheat, a QTL QTrl.saw-2D.2 associated with the total root length was identified, presumably containing genes closely related to root development. A mapping population of 184 recombinant inbred lines derived from the cross SY95-71 × CH7034 was used to map QTL for seedling root characteristics in hydroponic culture (HC) and in soil-filled pot (SP) methods. Four traits, including maximum root length (MRL), root number (RN), total length (TRL), and root diameter (RD) were measured and used in QTL analyses. A total of 33 QTL for the four root traits were detected, 17 QTLs for TRL, six for RN, seven for MRL, and three for RD. Seven QTL were detected in both HC and SP methods, which explained 7-18% phenotypic variation. One QTL QTrl.saw-2D.2 detected in both HC and SP methods was also validated in another population comprised of 215 diverse lines. This QTL is a novel QTL that explained the highest phenotypic variation 18% in all QTL identified in the present study. Based on candidate gene and comparative genomics analyses, the QTL QTrl.saw-2D.2 may contain genes closely related to root development in wheat (Triticum aestivum L.). The two candidate genes were proposed to explore in future studies.
MAIN CONCLUSION: In wheat, a QTL QTrl.saw-2D.2 associated with the total root length was identified, presumably containing genes closely related to root development. A mapping population of 184 recombinant inbred lines derived from the cross SY95-71 × CH7034 was used to map QTL for seedling root characteristics in hydroponic culture (HC) and in soil-filled pot (SP) methods. Four traits, including maximum root length (MRL), root number (RN), total length (TRL), and root diameter (RD) were measured and used in QTL analyses. A total of 33 QTL for the four root traits were detected, 17 QTLs for TRL, six for RN, seven for MRL, and three for RD. Seven QTL were detected in both HC and SP methods, which explained 7-18% phenotypic variation. One QTL QTrl.saw-2D.2 detected in both HC and SP methods was also validated in another population comprised of 215 diverse lines. This QTL is a novel QTL that explained the highest phenotypic variation 18% in all QTL identified in the present study. Based on candidate gene and comparative genomics analyses, the QTL QTrl.saw-2D.2 may contain genes closely related to root development in wheat (Triticum aestivum L.). The two candidate genes were proposed to explore in future studies.
Authors: Kai P Voss-Fels; Hannah Robinson; Stephen R Mudge; Cecile Richard; Saul Newman; Benjamin Wittkop; Andreas Stahl; Wolfgang Friedt; Matthias Frisch; Iulian Gabur; Anika Miller-Cooper; Bradley C Campbell; Alison Kelly; Glen Fox; Jack Christopher; Mandy Christopher; Karine Chenu; Jerome Franckowiak; Emma S Mace; Andrew K Borrell; Howard Eagles; David R Jordan; José R Botella; Graeme Hammer; Ian D Godwin; Ben Trevaskis; Rod J Snowdon; Lee T Hickey Journal: Mol Plant Date: 2017-10-19 Impact factor: 13.164
Authors: Jonathan A Atkinson; Luzie U Wingen; Marcus Griffiths; Michael P Pound; Oorbessy Gaju; M John Foulkes; Jacques Le Gouis; Simon Griffiths; Malcolm J Bennett; Julie King; Darren M Wells Journal: J Exp Bot Date: 2015-03-04 Impact factor: 6.992